Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
July-2016 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2016 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction

  • Authors:
    • Qi Zhao
    • Yansheng Xue
    • Yi Yang
    • Zhibin Niu
    • Changlin Wang
    • Ying Hou
    • Hui Chen
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Pediatric Surgery, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 681-688
    |
    Published online on: May 24, 2016
       https://doi.org/10.3892/mmr.2016.5338
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Renal fibrosis, considered to be a common consequence of progressive renal disease, involves glomerulosclerosis and/or tubulointerstitial fibrosis. Currently, research is focused on investigating potential mechanisms to prevent or reverse the damage caused by fibrosis. Under the influence of cytokines, chemokines and other signaling molecules, the cellular interactions that regulate the development of interstitial fibrosis are complex. Epithelial‑mesenchymal transition (EMT) has emerged as an important pathway leading to the generation of matrix‑producing fibroblasts and myofibroblasts in diseased kidneys. The proteomics study compared the protein profiles between the time points of podocyte EMT and tubular cell EMT in a partial unilateral ureteral obstruction (PUUO) model in rats. Proteins isolated from the PUUO group and corresponding sham rat kidney tissues were subjected to 2‑D gel electrophoresis and were then identified by mass spectrometry. In total, 43 proteins with differential expression were identified, which were reported to be involved in the regulation of the cytoskeleton and actin, glucose metabolism, cell apoptosis, mitochondrial energy metabolism, oxidative stress and endoplasmic reticulum stress. Electron transfer flavoprotein, β polypeptide was detected by immunoblot analysis and its mRNA levels were determined in renal tissues. The results demonstrate protein alterations that reflect the pathology of the obstructed kidneys, and thus may aid in understanding the pathogenesis of obstructive nephropathy.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Chevalier RL and Peters CA: Congenital urinary tract obstruction: Proceedings of the State-Of-The-Art Strategic Planning Workshop-National Institutes of Health Bethesda, Maryland, USA 11–12 March 2002. Pediatr Nephrol. 18:576–606. 2003.PubMed/NCBI

2 

Eddy AA: Molecular basis of renal fibrosis. Pediatr Nephrol. 15:290–301. 2000. View Article : Google Scholar

3 

Madsen MG, Nørregaard R, Frøkiær J and Jørgensen TM: Urinary biomarkers in prenatally diagnosed unilateral hydronephrosis. J Pediatr Urol. 7:105–112. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Wen JG, Ringgaard S, Jorgensen TM, Stodkilde-Jorgensen H, Djurhuus JC and Frokiaer J: Long-term effects of partial unilateral ureteral obstruction on renal hemodynamics and morphology in newborn rats: a magnetic resonance imaging study. Urol Res. 30:205–212. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Liu Y: New insights into epithelial-mesenchymal transition in kidney fibrosis. J Am Soc Nephrol. 21:212–222. 2010. View Article : Google Scholar

6 

Chevalier RL: Obstructive nephropathy: Towards biomarker discovery and gene therapy. Nat Clin Pract Nephrol. 2:157–168. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Ulm AH and Miller F: An operation to produce experimental reversible hydronephrosis in dogs. J Urol. 88:337–341. 1962.PubMed/NCBI

8 

Zhao Q, Yang Y, Wang CL, Hou Y and Chen H: Screening and identification of the differential proteins in kidney with complete unilateral ureteral obstruction. Int J Clin Exp Pathol. 8:2615–2626. 2015.PubMed/NCBI

9 

Pappin DJ, Hojrup P and Bleasby AJ: Rapid identification of proteins by peptide-mass fingerprinting. Curr Biol. 3:327–332. 1993. View Article : Google Scholar : PubMed/NCBI

10 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

11 

Li Y, Kang YS, Dai C, Kiss LP, Wen X and Liu Y: Epithelial-to-mesenchymal transition is a potential pathway leading to podocyte dysfunction and proteinuria. Am J Pathol. 172:299–308. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Thornhill BA, Burt LE, Chen C, Forbes MS and Chevalier RL: Variable chronic partial ureteral obstruction in the neonatal rat: A new model of ureteropelvic junction obstruction. Kidney Int. 67:42–52. 2005. View Article : Google Scholar

13 

Seseke F, Thelen P, Heuser M, Zöller G and Ringert RH: Impaired nephrogenesis in rats with congenital obstructive uropathy. J Urol. 165:2289–2292. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Yu XX, Wang CL, Sun RG, et al: Renal pathological appearance and podocyte phenotype after the ureteral obstruction. Journal of China Medical University. 39:1–3. 2010.

15 

Dominguez R and Holmes KC: Actin structure and function. Annu Rev Biophys. 40:169–186. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Small DM, Coombes JS, Bennett N, Johnson DW and Gobe GC: Oxidative stress, anti-oxidant therapies and chronic kidney disease. Nephrology (Carlton). 17:311–321. 2012. View Article : Google Scholar

17 

Hebert DN and Molinari M: In and out of the ER: Protein folding, quality control, degradation, and related human diseases. Physiol Rev. 87:1377–1408. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Ellgaard L, Molinari M and Helenius A: Setting the standards: Quality control in the secretory pathway. Science. 286:1882–1888. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Dickhout JG, Carlisle RE and Austin RC: Interrelationship between cardiac hypertrophy, heart failure, and chronic kidney disease: Endoplasmic reticulum stress as a mediator of pathogenesis. Circ Res. 108:629–642. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Salahudeen AK, Huang H, Joshi M, Moore NA and Jenkins JK: Involvement of the mitochondrial pathway in cold storage and rewarming-associated apoptosis of human renal proximal tubular cells. Am J Transplant. 3:273–280. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Manucha W, Kurbán F, Mazzei L, Benardón ME, Bocanegra V, Tosi MR and Vallés P: eNOS/Hsp70 interaction on rosuvastatin cytoprotective effect in neonatal obstructive nephropathy. Eur J Pharmacol. 650:487–495. 2011. View Article : Google Scholar

22 

Lodha S, Dani D, Mehta R, Bhaskaran M, Reddy K, Ding G and Singhal PC: Angiotensin II-induced mesangial cell apoptosis: Role of oxidative stress. Mol Med. 8:830–840. 2002.

23 

Frerman FE: Acyl-CoA dehydrogenases, electron transfer flavoprotein and electron transfer flavoprotein dehydrogenase. Biochem Soc Trans. 16:416–418. 1988. View Article : Google Scholar : PubMed/NCBI

24 

Beckmann JD and Frerman FE: Electron-transfer flavoprotein-ubiquinone oxidoreductase from pig liver: Purification and molecular, redox, and catalytic properties. Biochemistry. 24:3913–3921. 1985. View Article : Google Scholar : PubMed/NCBI

25 

Hirokawa S, Shimanuki T, Kitajima H, Nishimori Y and Shimosaka M: Identification of ETFB as a candidate protein that participates in the mechanoregulation of fibroblast cell number in collagen gel culture. J Dermatol Sci. 64:119–126. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Hirokawa S, Shimanuki T, Kitajima H, Nishimori Y and Shimosaka M: Knockdown of electron transfer flavoprotein β subunit reduced TGF-β-induced α-SMA mRNA expression but not COL1A1 in fibroblast-populated three-dimensional collagen gel cultures. J Dermatol Sci. 68:179–186. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhao Q, Xue Y, Yang Y, Niu Z, Wang C, Hou Y and Chen H: Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction. Mol Med Rep 14: 681-688, 2016.
APA
Zhao, Q., Xue, Y., Yang, Y., Niu, Z., Wang, C., Hou, Y., & Chen, H. (2016). Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction. Molecular Medicine Reports, 14, 681-688. https://doi.org/10.3892/mmr.2016.5338
MLA
Zhao, Q., Xue, Y., Yang, Y., Niu, Z., Wang, C., Hou, Y., Chen, H."Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction". Molecular Medicine Reports 14.1 (2016): 681-688.
Chicago
Zhao, Q., Xue, Y., Yang, Y., Niu, Z., Wang, C., Hou, Y., Chen, H."Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction". Molecular Medicine Reports 14, no. 1 (2016): 681-688. https://doi.org/10.3892/mmr.2016.5338
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Q, Xue Y, Yang Y, Niu Z, Wang C, Hou Y and Chen H: Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction. Mol Med Rep 14: 681-688, 2016.
APA
Zhao, Q., Xue, Y., Yang, Y., Niu, Z., Wang, C., Hou, Y., & Chen, H. (2016). Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction. Molecular Medicine Reports, 14, 681-688. https://doi.org/10.3892/mmr.2016.5338
MLA
Zhao, Q., Xue, Y., Yang, Y., Niu, Z., Wang, C., Hou, Y., Chen, H."Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction". Molecular Medicine Reports 14.1 (2016): 681-688.
Chicago
Zhao, Q., Xue, Y., Yang, Y., Niu, Z., Wang, C., Hou, Y., Chen, H."Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction". Molecular Medicine Reports 14, no. 1 (2016): 681-688. https://doi.org/10.3892/mmr.2016.5338
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team