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Article

FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats

  • Authors:
    • Chuang Huang
    • Rui Ling
    • Fei‑Jiang Li
    • Er‑Cui Li
    • Qi‑Ke Huang
    • Bao‑Gang Liu
    • Yin Ding
    • Si‑Wei You
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of General Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Biomedical Engineering, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Gastroenterology and Endocrinology, Shaanxi Provincial Armed Police Corps Hospital, Xi'an, Shaanxi 710032, P.R. China, Out‑Patient Department, General Hospital of The Second Artillery, Beijing 100820, P.R. China, Department of Ophthalmology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 927-935
    |
    Published online on: May 24, 2016
       https://doi.org/10.3892/mmr.2016.5342
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Abstract

Sphingosine-1-phosphate and its structural analog FTY720 (fingolimod) are important in the inhibition of osteoclast differentiation and bone resorption, however, it remains unknown whether they enhance osteogenic differentiation of the bone marrow mesenchymal stem cells (BM‑MSCs). The present study investigated the effect of FTY720 on the osteogenic differentiation of BM‑MSCs from the femurs of the ovariectomized (OVX) rats. Three different concentrations (1, 10 and 100 nM) of FTY720 were demonstrated to markedly upregulate mRNA expression levels of Runt‑related transcription factor 2 (Runx2) and Sp7 transcription factor (Sp7) at 2 weeks, and alkaline phosphatase (ALP) at 3 weeks. The osteocalcin (OCN) expression was similar at weeks 2 and 3. The protein expression levels of Runx2, Sp7, OCN and ALP induced by three different concentrations of FTY720 were higher than those in the control groups at 3 weeks in the OVX and sham groups. The findings of the current study suggested a beneficial effect of FTY720 on bone formation in OVX rats, and provided a potential therapeutic method of FTY720 to prevent alveolar bone resorption in patients with post‑menopausal osteoporosis.
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Copy and paste a formatted citation
Spandidos Publications style
Huang C, Ling R, Li FJ, Li EC, Huang QK, Liu BG, Ding Y and You SW: FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats. Mol Med Rep 14: 927-935, 2016.
APA
Huang, C., Ling, R., Li, F., Li, E., Huang, Q., Liu, B. ... You, S. (2016). FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats. Molecular Medicine Reports, 14, 927-935. https://doi.org/10.3892/mmr.2016.5342
MLA
Huang, C., Ling, R., Li, F., Li, E., Huang, Q., Liu, B., Ding, Y., You, S."FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats". Molecular Medicine Reports 14.1 (2016): 927-935.
Chicago
Huang, C., Ling, R., Li, F., Li, E., Huang, Q., Liu, B., Ding, Y., You, S."FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats". Molecular Medicine Reports 14, no. 1 (2016): 927-935. https://doi.org/10.3892/mmr.2016.5342
Copy and paste a formatted citation
x
Spandidos Publications style
Huang C, Ling R, Li FJ, Li EC, Huang QK, Liu BG, Ding Y and You SW: FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats. Mol Med Rep 14: 927-935, 2016.
APA
Huang, C., Ling, R., Li, F., Li, E., Huang, Q., Liu, B. ... You, S. (2016). FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats. Molecular Medicine Reports, 14, 927-935. https://doi.org/10.3892/mmr.2016.5342
MLA
Huang, C., Ling, R., Li, F., Li, E., Huang, Q., Liu, B., Ding, Y., You, S."FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats". Molecular Medicine Reports 14.1 (2016): 927-935.
Chicago
Huang, C., Ling, R., Li, F., Li, E., Huang, Q., Liu, B., Ding, Y., You, S."FTY720 enhances osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomized rats". Molecular Medicine Reports 14, no. 1 (2016): 927-935. https://doi.org/10.3892/mmr.2016.5342
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