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Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells

  • Authors:
    • Lin Liu
    • Dian Wang
    • Longlong Li
    • Xiao Ding
    • Haitian Ma
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 705-714
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    Published online on: May 24, 2016
       https://doi.org/10.3892/mmr.2016.5343
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Abstract

Dehydroepiandrosterone (DHEA) is widely used as a nutritional supplement and exhibits putative anti‑aging properties. However, the molecular basis of the actions of DHEA, particularly on the biological characteristics of target cells, remain unclear. The aim of the current study was to investigate the effects of DHEA on cell viability, cell proliferation, cell cycle and mitochondrial function in primary rat Leydig cells. Adult Leydig cells were purified by Percoll gradient centrifugation, and cell proliferation was detected using a Click-iT® EdU Assay kit and cell cycle assessment performed using flow cytometry. Mitochondrial membrane potential was detected using JC-1 staining assay. The results of the current study demonstrate that DHEA decreased cell proliferation in a dose‑dependent manner, whereas it improved cell viability in a time‑dependent and dose‑dependent manner. Flow cytometry analysis demonstrated that DHEA treatment increased the S phase cell population and decreased the G2/M cell population. Cyclin A and CDK2 mRNA levels were decreased in primary rat Leydig cells following DHEA treatment. DHEA treatment decreased the transmembrane electrical gradient in primary Leydig cells, whereas treatment significantly increased succinate dehydrogenase activity. These results indicated that DHEA inhibits primary rat Leydig cell proliferation by decreasing cyclin mRNA level, whereas it improves cells viability by modulating the permeability of the mitochondrial membrane and succinate dehydrogenase activity. These findings may demonstrate an important molecular mechanism by which DHEA activity is mediated.
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Copy and paste a formatted citation
Spandidos Publications style
Liu L, Wang D, Li L, Ding X and Ma H: Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells. Mol Med Rep 14: 705-714, 2016.
APA
Liu, L., Wang, D., Li, L., Ding, X., & Ma, H. (2016). Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells. Molecular Medicine Reports, 14, 705-714. https://doi.org/10.3892/mmr.2016.5343
MLA
Liu, L., Wang, D., Li, L., Ding, X., Ma, H."Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells". Molecular Medicine Reports 14.1 (2016): 705-714.
Chicago
Liu, L., Wang, D., Li, L., Ding, X., Ma, H."Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells". Molecular Medicine Reports 14, no. 1 (2016): 705-714. https://doi.org/10.3892/mmr.2016.5343
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Wang D, Li L, Ding X and Ma H: Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells. Mol Med Rep 14: 705-714, 2016.
APA
Liu, L., Wang, D., Li, L., Ding, X., & Ma, H. (2016). Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells. Molecular Medicine Reports, 14, 705-714. https://doi.org/10.3892/mmr.2016.5343
MLA
Liu, L., Wang, D., Li, L., Ding, X., Ma, H."Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells". Molecular Medicine Reports 14.1 (2016): 705-714.
Chicago
Liu, L., Wang, D., Li, L., Ding, X., Ma, H."Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells". Molecular Medicine Reports 14, no. 1 (2016): 705-714. https://doi.org/10.3892/mmr.2016.5343
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