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August-2016 Volume 14 Issue 2

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Article

Adenovirus with p16 gene exerts antitumor effect on laryngeal carcinoma Hep2 cells

  • Authors:
    • Zhengang Yang
    • Jingxia Hu
    • Dajun Li
    • Xinliang Pan
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Institute of Immunology, Taishan Medical University, Tai'an, Shandong 271100, P.R. China, Department of Otolaryngology, Tai'an Central Hospital, Tai'an, Shandong 271000, P.R. China
  • Pages: 1425-1429
    |
    Published online on: June 1, 2016
       https://doi.org/10.3892/mmr.2016.5355
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Abstract

Laryngeal cancer is an uncommon form of cancer. The tumor suppressor P16, known to be mutated or deleted in various types of human tumor, including laryngeal carcinoma, is involved in the formation and development of laryngeal carcinoma. It has been previously reported that the inactivation or loss of P16 is associated with the acquisition of malignant characteristics. The current study hypothesized that restoring wild‑type P16 activity into P16‑null malignant Hep2 cells may exert an antitumor effect. A recombinant adenovirus carrying the P16 gene (Ad‑P16) was used to infect and express high levels of P16 protein in P16‑null Hep2 cells. Cell proliferation and invasion assays and polymerase chain reaction were performed to evaluate the effects of the P16 gene on cell proliferation and the antitumor effect on Hep2 cells. The results demonstrated that the Hep2 cells infected with Ad‑P16 exhibited significantly reduced cell proliferation, invasion and tumor volume compared with untreated or control adenovirus cells. Furthermore, the expression of laryngeal carcinoma‑associated genes, EGFR, survivin and cyclin D1, were measured in Ad‑P16‑infected cells and were significantly reduced compared with control groups. The results of the current study demonstrate that restoring wild‑type P16 activity into P16-null Hep2 cells exerts an antitumor effect.
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1 

Jemal A, Clegg LX, Ward E, Ries LA, Wu X, Jamison PM, Wingo PA, Howe HL, Anderson RN and Edwards BK: Annual report to the nation on the status of cancer, 1975–2001, with a special feature regarding survival. Cancer. 101:3–27. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. Ca: Cancer J Clin. 65:5–29. 2015.

3 

Weinberg RA: pRb and Control of the Cell Cycle Clock In: The Biology of Cancer. 2nd edition. Garland Science; New York, NY: pp. 275–329. 2014

4 

Liggett WH Jr and Sidransky D: Role of the p16 tumor suppressor gene in cancer. J Clin Oncol. 16:1197–1206. 1998.PubMed/NCBI

5 

Attri J, Srinivasan R, Majumdar S, Radotra BD and Wig J: Alterations of tumor suppressor gene p16INK4a in pancreatic ductal carcinoma. BMC Gastroenterol. 5:222005. View Article : Google Scholar : PubMed/NCBI

6 

Yuen PW, Man M, Lam KY and Kwong YL: Clinicopathological significance of p16 gene expression in the surgical treatment of head and neck squamous cell carcinomas. J Clin Pathol. 55:58–60. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Kalfert D, Celakovsky P, Laco J and Ludvikova M: The role of protein p16 (INK4a) in glottic laryngeal squamous cell carcinoma. Pathol Oncol Res. 20:909–915. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Craig C, Kim M, Ohri E, Wersto R, Katayose D, Li Z, Choi YH, Mudahar B, Srivastava S, Seth P and Cowan K: Effects of adenovirus-mediated p16INK4A expression on cell cycle arrest are determined by endogenous p16 and Rb status in human cancer cells. Oncogene. 16:265–272. 1998. View Article : Google Scholar : PubMed/NCBI

9 

Piao CQ, Zhao YL and Hei TK: Analysis of p16 and p21 (Cip1) expression in tumorigenic human bronchial epithelial cells induced by asbestos. Oncogene. 20:7301–7306. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Li X, Zhu J, Man Z, Ao Y and Chen H: Investigation on the structure and upconversion fluorescence of Yb3+/Ho3+ co-doped fluorapatite crystals for potential biomedical applications. Sci Rep. 4:44462014.

11 

Ma J, He X, Wang W, Huang Y, Chen L, Cong W, Gu J, Hu H, Shi J, Li L and Su C: E2F promoter-regulated oncolytic adenovirus with p16 gene induces cell apoptosis and exerts antitumor effect on gastric cancer. Dig Dis Sci. 54:1425–1431. 2009. View Article : Google Scholar

12 

Chintala SK, Fueyo J, Gomez-Manzano C, Venkaiah B, Bjerkvig R, Yung WK, Sawaya R, Kyritsis AP and Rao JS: Adenovirus-mediated p16/CDKN2 gene transfer suppresses glioma invasion in vitro. Oncogene. 15:2049–2057. 1997. View Article : Google Scholar : PubMed/NCBI

13 

Knight BB, Oprea-Ilies GM, Nagalingam A, Yang L, Cohen C, Saxena NK and Sharma D: Survivin upregulation, dependent on leptin-EGFR-Notch1 axis, is essential for leptin-induced migration of breast carcinoma cells. Endocr Relat Cancer. 18:413–428. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408

15 

Huang YP, Lin IJ, Chen CC, Hsu YC, Chang CC and Lee MJ: Delivery of small interfering RNAs in human cervical cancer cells by polyethylenimine-functionalized carbon nanotubes. Nanoscale Res Lett. 8:2672013. View Article : Google Scholar : PubMed/NCBI

16 

O'Neill CJ and McCluggage WG: p16 expression in the female genital tract and its value in diagnosis. Adv Anat Pathol. 13:8–15. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Villacañas O, Pérez JJ and Rubio-Martínez J: Structural analysis of the inhibition of Cdk4 and Cdk6 by p16 (INK4a) through molecular dynamics simulations. J Biomol Struct Dyn. 20:347–358. 2002. View Article : Google Scholar

18 

Nalabothula N, Lakka SS, Dinh DH, Gujrati M, Olivero WC and Rao JS: Sense p16 and antisense uPAR bicistronic construct inhibits angiogenesis and induces glioma cell death. Int J Oncol. 30:669–678. 2007.PubMed/NCBI

19 

Straume O, Sviland L and Akslen LA: Loss of nuclear p16 protein expression correlates with increased tumor cell proliferation (Ki-67) and poor prognosis in patients with vertical growth phase melanoma. Clin Cancer Res. 6:1845–1853. 2000.PubMed/NCBI

20 

Liang J, Wang P, Xie S, Wang W, Zhou X, Hu J, Shi Q, Zhang X and Yu R: Bmi-1 regulates the migration and invasion of glioma cells through p16. Cell Biol Int. 39:283–290. 2015. View Article : Google Scholar

21 

Maurizi M, Almadori G, Ferrandina G, Distefano M, Romanini ME, Cadoni G, Benedetti-Panici P, Paludetti G, Scambia G and Mancuso S: Prognostic significance of epidermal growth factor receptor in laryngeal squamous cell carcinoma. Br J Cancer. 74:1253–1257. 1996. View Article : Google Scholar : PubMed/NCBI

22 

Lionello M, Lovato A, Staffieri A, Blandamura S, Turato C, Giacomelli L, Staffieri C and Marioni G: The EGFR-mTOR pathway and laryngeal cancer angiogenesis. Eur Arch Otorhinolaryngol. 271:757–764. 2014. View Article : Google Scholar

23 

Fukuda S and Pelus LM: Survivin, a cancer target with an emerging role in normal adult tissues. Mol Cancer Ther. 5:1087–1098. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Alao JP: The regulation of cyclin D1 degradation: Roles in cancer development and the potential for therapeutic invention. Mol Cancer. 6:242007. View Article : Google Scholar : PubMed/NCBI

25 

Papadimitrakopoulou V, Izzo JG, Liu DD, Myers J, Ceron TL, Lewin J, William WN Jr, Atwell A, Lee JJ, Gillenwater A, et al: Cyclin D1 and cancer development in laryngeal premalignancy patients. Cancer Prev Res (Phila). 2:14–21. 2009. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yang Z, Hu J, Li D and Pan X: Adenovirus with p16 gene exerts antitumor effect on laryngeal carcinoma Hep2 cells. Mol Med Rep 14: 1425-1429, 2016.
APA
Yang, Z., Hu, J., Li, D., & Pan, X. (2016). Adenovirus with p16 gene exerts antitumor effect on laryngeal carcinoma Hep2 cells. Molecular Medicine Reports, 14, 1425-1429. https://doi.org/10.3892/mmr.2016.5355
MLA
Yang, Z., Hu, J., Li, D., Pan, X."Adenovirus with p16 gene exerts antitumor effect on laryngeal carcinoma Hep2 cells". Molecular Medicine Reports 14.2 (2016): 1425-1429.
Chicago
Yang, Z., Hu, J., Li, D., Pan, X."Adenovirus with p16 gene exerts antitumor effect on laryngeal carcinoma Hep2 cells". Molecular Medicine Reports 14, no. 2 (2016): 1425-1429. https://doi.org/10.3892/mmr.2016.5355
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Z, Hu J, Li D and Pan X: Adenovirus with p16 gene exerts antitumor effect on laryngeal carcinoma Hep2 cells. Mol Med Rep 14: 1425-1429, 2016.
APA
Yang, Z., Hu, J., Li, D., & Pan, X. (2016). Adenovirus with p16 gene exerts antitumor effect on laryngeal carcinoma Hep2 cells. Molecular Medicine Reports, 14, 1425-1429. https://doi.org/10.3892/mmr.2016.5355
MLA
Yang, Z., Hu, J., Li, D., Pan, X."Adenovirus with p16 gene exerts antitumor effect on laryngeal carcinoma Hep2 cells". Molecular Medicine Reports 14.2 (2016): 1425-1429.
Chicago
Yang, Z., Hu, J., Li, D., Pan, X."Adenovirus with p16 gene exerts antitumor effect on laryngeal carcinoma Hep2 cells". Molecular Medicine Reports 14, no. 2 (2016): 1425-1429. https://doi.org/10.3892/mmr.2016.5355
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