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Article

RUNX2 controls human IPO8 basal transcription in Saos-2 cells

  • Authors:
    • Jianjun Xiong
    • Zhihong Hu
    • Ting Wang
    • Xiaoyuan Xu
    • Jianyun Liu
    • Ping Wu
    • Xiangxin Che
    • Weidong Li
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi 332000, P.R. China, Key Laboratory of Jiangxi for the Systems Bio‑medicine, Jiujiang University, Jiujiang, Jiangxi 332000, P.R. China
  • Pages: 1418-1424
    |
    Published online on: June 1, 2016
       https://doi.org/10.3892/mmr.2016.5356
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Abstract

Runt-related transcription factor 2 (RUNX2) is a vital regulatory factor that controls osteoblast-specific gene expression; however, RUNX2‑regulated genes in human mesenchymal stem cells (hMSCs) remain to be fully elucidated. In the present study, chromatin immunoprecipitation (ChIP)-on-chip analysis of RUNX2 in hMSCs demonstrated that importin 8 (IPO8) may be a novel target gene. The 5' flanking region of the IPO8 gene, which is ~3,300 bp in length, was cloned and inserted into the pGL3‑basic luciferase reporter vector. The results of dual luciferase reporter assays indicated that this segment possessed strong basal promoter activity. Furthermore, the RUNX2 binding site, which encompasses positions ‑496 to ‑501 bp, was required to achieve maximal IPO8 promoter activity in Saos‑2 human osteosarcoma cells. In addition, ChIP analysis indicated that RUNX2 uniquely binds to this specific IPO8 sequence motif. Cells with a knockdown in RUNX2 expression exhibited downregulated IPO8 transcription. Finally, synchronization of IPO8 and RUNX2 expression was observed in Saos‑2 cells cultured in osteoblast‑induction medium. Taken together, these results indicated that RUNX2 regulates IPO8 gene transcription, and may have a contributory role in osteoblast differentiation.
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1 

Bruder SP, Jaiswal N and Haynesworth SE: Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem. 64:278–294. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Engler AJ, Sen S, Sweeney HL and Discher DE: Matrix elasticity directs stem cell lineage specification. Cell. 126:677–689. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Geoffroy V, Kneissel M, Fournier B, Boyde A and Matthias P: High bone resorption in adult aging transgenic mice overexpressing cbfa1/runx2 in cells of the osteoblastic lineage. Mol Cell Biol. 22:6222–6233. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Shui C, Spelsberg TC, Riggs BL and Khosla S: Changes in Runx2/Cbfa1 expression and activity during osteoblastic differentiation of human bone marrow stromal cells. J Bone Miner Res. 18:213–221. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Ducy P, Zhang R, Geoffroy V, Ridall AL and Karsenty G: Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation. Cell. 89:747–754. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Teplyuk NM, Haupt LM, Ling L, Dombrowski C, Mun FK, Nathan SS, Lian JB, Stein JL, Stein GS, Cool SM and van Wijnen AJ: The osteogenic transcription factor Runx2 regulates components of the fibroblast growth factor/proteoglycan signaling axis in osteoblasts. J Cell Biochem. 107:144–154. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Stewart M: Molecular mechanism of the nuclear protein import cycle. Nat Rev Mol Cell Biol. 8:195–208. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Kutay U, Bischoff FR, Kostka S, Kraft R and Görlich D: Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor. Cell. 90:1061–1071. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Cook A, Bono F, Jinek M and Conti E: Structural biology of nucleocytoplasmic transport. Annu Rev Biochem. 76:647–671. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Görlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, Prehn S and Izaurralde E: A novel class of RanGTP binding proteins. J Cell Biol. 138:65–80. 1997. View Article : Google Scholar : PubMed/NCBI

11 

Nguewa PA, Agorreta J, Blanco D, Lozano MD, Gomez-Roman J, Sanchez BA, Valles I, Pajares MJ, Pio R, Rodriguez MJ, et al: Identification of importin 8 (IPO8) as the most accurate reference gene for the clinicopathological analysis of lung specimens. BMC Mol Biol. 9:1032008. View Article : Google Scholar : PubMed/NCBI

12 

Kreth S, Heyn J, Grau S, Kretzschmar HA, Egensperger R and Kreth FW: Identification of valid endogenous control genes for determining gene expression in human glioma. Neuro Oncol. 12:570–579. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Hurtado del Pozo C, Calvo RM, Vesperinas-García G, Gómez-Ambrosi J, Frühbeck G, Corripio-Sánchez R, Rubio MA and Obregon MJ: IPO8 and FBXL10: New reference genes for gene expression studies in human adipose tissue. Obesity (Silver Spring). 18:897–903. 2010. View Article : Google Scholar

14 

Weinmann L, Höck J, Ivacevic T, Ohrt T, Mütze J, Schwille P, Kremmer E, Benes V, Urlaub H and Meister G: Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. Cell. 136:496–507. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

16 

Komori T: Regulation of skeletal development by the Runx family of transcription factors. J Cell Biochem. 95:445–453. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Little GH, Noushmehr H, Baniwal SK, Berman BP, Coetzee GA and Frenkel B: Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion. Nucleic Acids Res. 40:3538–3547. 2012. View Article : Google Scholar :

18 

Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, et al: Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell. 89:755–764. 1997. View Article : Google Scholar : PubMed/NCBI

19 

Nishio Y, Dong Y, Paris M, O'Keefe RJ, Schwarz EM and Drissi H: Runx2-mediated regulation of the zinc finger Osterix/Sp7 gene. Gene. 372:62–70. 2006. View Article : Google Scholar : PubMed/NCBI

20 

van der Deen M, Akech J, Lapointe D, Gupta S, Young DW, Montecino MA, Galindo M, Lian JB, Stein JL, Stein GS and van Wijnen AJ: Genomic promoter occupancy of runt-related transcription factor RUNX2 in Osteosarcoma cells identifies genes involved in cell adhesion and motility. J Biol Chem. 287:4503–4517. 2012. View Article : Google Scholar :

21 

Dean KA, von Ahsen O, Görlich D and Fried HM: Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin. J Cell Sci. 114:3479–3485. 2001.PubMed/NCBI

22 

Xu L, Yao X, Chen X, Lu P, Zhang B and Ip YT: Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads. J Cell Biol. 178:981–994. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Freedman ND and Yamamoto KR: Importin 7 and importin alpha/importin beta are nuclear import receptors for the gluco-corticoid receptor. Mol Biol Cell. 15:2276–2286. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Derynck R and Zhang YE: Smad-dependent and Smad-independent pathways in TGF-beta family signalling. Nature. 425:577–584. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Retting KN, Song B, Yoon BS and Lyons KM: BMP canonical Smad signaling through Smad1 and Smad5 is required for endochondral bone formation. Development. 136:1093–1104. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xiong J, Hu Z, Wang T, Xu X, Liu J, Wu P, Che X and Li W: RUNX2 controls human IPO8 basal transcription in Saos-2 cells. Mol Med Rep 14: 1418-1424, 2016.
APA
Xiong, J., Hu, Z., Wang, T., Xu, X., Liu, J., Wu, P. ... Li, W. (2016). RUNX2 controls human IPO8 basal transcription in Saos-2 cells. Molecular Medicine Reports, 14, 1418-1424. https://doi.org/10.3892/mmr.2016.5356
MLA
Xiong, J., Hu, Z., Wang, T., Xu, X., Liu, J., Wu, P., Che, X., Li, W."RUNX2 controls human IPO8 basal transcription in Saos-2 cells". Molecular Medicine Reports 14.2 (2016): 1418-1424.
Chicago
Xiong, J., Hu, Z., Wang, T., Xu, X., Liu, J., Wu, P., Che, X., Li, W."RUNX2 controls human IPO8 basal transcription in Saos-2 cells". Molecular Medicine Reports 14, no. 2 (2016): 1418-1424. https://doi.org/10.3892/mmr.2016.5356
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong J, Hu Z, Wang T, Xu X, Liu J, Wu P, Che X and Li W: RUNX2 controls human IPO8 basal transcription in Saos-2 cells. Mol Med Rep 14: 1418-1424, 2016.
APA
Xiong, J., Hu, Z., Wang, T., Xu, X., Liu, J., Wu, P. ... Li, W. (2016). RUNX2 controls human IPO8 basal transcription in Saos-2 cells. Molecular Medicine Reports, 14, 1418-1424. https://doi.org/10.3892/mmr.2016.5356
MLA
Xiong, J., Hu, Z., Wang, T., Xu, X., Liu, J., Wu, P., Che, X., Li, W."RUNX2 controls human IPO8 basal transcription in Saos-2 cells". Molecular Medicine Reports 14.2 (2016): 1418-1424.
Chicago
Xiong, J., Hu, Z., Wang, T., Xu, X., Liu, J., Wu, P., Che, X., Li, W."RUNX2 controls human IPO8 basal transcription in Saos-2 cells". Molecular Medicine Reports 14, no. 2 (2016): 1418-1424. https://doi.org/10.3892/mmr.2016.5356
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