Identification and characteristics of the testes-specific gene, Ccdc38, in mice

Lin,Shou‑Ren; Li,Yu‑Chi; Luo,Man‑Ling; Guo,Huan; Wang,Tian‑Tian; Chen,Jian‑Bo; Ma,Qian; Gu,Yan‑Li; Jiang,Zhi‑Mao; Gui,Yao‑Ting

doi:10.3892/mmr.2016.5360

August-2016 Volume 14 Issue 2

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August-2016 Volume 14 Issue 2

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Article

Identification and characteristics of the testes-specific gene, Ccdc38, in mice

Authors:
- Shou‑Ren Lin
- Yu‑Chi Li
- Man‑Ling Luo
- Huan Guo
- Tian‑Tian Wang
- Jian‑Bo Chen
- Qian Ma
- Yan‑Li Gu
- Zhi‑Mao Jiang
- Yao‑Ting Gui
View Affiliations / Copyright

Affiliations: Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China
Pages: 1290-1296
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Published online on: June 3, 2016

https://doi.org/10.3892/mmr.2016.5360
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Abstract

Distinguishing the testes-speciﬁc genes in different species may disclose key genes associated with testes-speciﬁc functions and provide sufficient information for the study and treatment of male infertility. A testes‑specific gene, coiled-coil domain containing 38 (Ccdc38), was identified by screening UniGene libraries. Systematic bioinformatics analysis demonstrated that the CCDC38 protein was conserved in various mammalian species. It was determined that CCDC38 was exclusively expressed in testes and its expression increased from 2‑8 weeks of age. Additional immunohistochemical analysis indicated that CCDC38 was mainly expressed in spermatogonia and spermatocytes. It is of note that, immunofluorescence and co-immunoprecipitation assays demonstrated that CCDC38 interacted with ubiquitinated histone H2A in mouse testes. Therefore, these results suggest that Ccdc38 is a testes-specific gene, which may be important for mouse spermatogenesis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Identification and characteristics of the testes-specific gene, Ccdc38, in mice

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