MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo

  • Authors:
    • Hong‑Li Li
    • Hui Wu
    • Bei‑Bei Zhang
    • Hai‑Lian Shi
    • Xiao‑Jun Wu
  • View Affiliations

  • Published online on: June 3, 2016     https://doi.org/10.3892/mmr.2016.5361
  • Pages: 1430-1438
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Abstract

Gastric cancer is the second leading cause of cancer-associated mortality worldwide. This investigation aimed to identify whether the mitogen‑activated protein kinase (MAPK) signaling pathways are involved in the inhibitory effect of berberine hydrochloride (BER) on MGC 803 cells in vitro and in vivo. BER time‑ and dose‑dependently inhibited proliferation of MGC 803 cells. It also suppressed tumorigenesis in nude mice xenografted with MGC 803 cells. Additionally, BER reduced interleukin‑8 (IL‑8) secretion in vitro and in vivo. Further investigation demonstrated that inactivation of p38 MAPK, extracellular-signal regulated kinase 1/2 and c‑Jun N‑terminal kinase by BER contributed to the decreased proliferation and tumorigenesis, and the change in IL‑8 expression levels. However, there was no significant synergistic inhibitory effect of combined BER and evodiamine (EVO) treatment on tumorigenesis, and BER reduced the upregulation of IL‑8 induced by EVO in vivo. The results of the current study suggested that BER may be an effective and safe drug candidate for treating gastric cancer via modulation of the MAPK signaling pathways.
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August-2016
Volume 14 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li HL, Wu H, Zhang BB, Shi HL and Wu XJ: MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo. Mol Med Rep 14: 1430-1438, 2016
APA
Li, H., Wu, H., Zhang, B., Shi, H., & Wu, X. (2016). MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo. Molecular Medicine Reports, 14, 1430-1438. https://doi.org/10.3892/mmr.2016.5361
MLA
Li, H., Wu, H., Zhang, B., Shi, H., Wu, X."MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo". Molecular Medicine Reports 14.2 (2016): 1430-1438.
Chicago
Li, H., Wu, H., Zhang, B., Shi, H., Wu, X."MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo". Molecular Medicine Reports 14, no. 2 (2016): 1430-1438. https://doi.org/10.3892/mmr.2016.5361