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Article

MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo

  • Authors:
    • Hong‑Li Li
    • Hui Wu
    • Bei‑Bei Zhang
    • Hai‑Lian Shi
    • Xiao‑Jun Wu
  • View Affiliations / Copyright

    Affiliations: Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica, The Ministry of Education Key Laboratory for Standardization of Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China
  • Pages: 1430-1438
    |
    Published online on: June 3, 2016
       https://doi.org/10.3892/mmr.2016.5361
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Abstract

Gastric cancer is the second leading cause of cancer-associated mortality worldwide. This investigation aimed to identify whether the mitogen‑activated protein kinase (MAPK) signaling pathways are involved in the inhibitory effect of berberine hydrochloride (BER) on MGC 803 cells in vitro and in vivo. BER time‑ and dose‑dependently inhibited proliferation of MGC 803 cells. It also suppressed tumorigenesis in nude mice xenografted with MGC 803 cells. Additionally, BER reduced interleukin‑8 (IL‑8) secretion in vitro and in vivo. Further investigation demonstrated that inactivation of p38 MAPK, extracellular-signal regulated kinase 1/2 and c‑Jun N‑terminal kinase by BER contributed to the decreased proliferation and tumorigenesis, and the change in IL‑8 expression levels. However, there was no significant synergistic inhibitory effect of combined BER and evodiamine (EVO) treatment on tumorigenesis, and BER reduced the upregulation of IL‑8 induced by EVO in vivo. The results of the current study suggested that BER may be an effective and safe drug candidate for treating gastric cancer via modulation of the MAPK signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Li HL, Wu H, Zhang BB, Shi HL and Wu XJ: MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo. Mol Med Rep 14: 1430-1438, 2016.
APA
Li, H., Wu, H., Zhang, B., Shi, H., & Wu, X. (2016). MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo. Molecular Medicine Reports, 14, 1430-1438. https://doi.org/10.3892/mmr.2016.5361
MLA
Li, H., Wu, H., Zhang, B., Shi, H., Wu, X."MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo". Molecular Medicine Reports 14.2 (2016): 1430-1438.
Chicago
Li, H., Wu, H., Zhang, B., Shi, H., Wu, X."MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo". Molecular Medicine Reports 14, no. 2 (2016): 1430-1438. https://doi.org/10.3892/mmr.2016.5361
Copy and paste a formatted citation
x
Spandidos Publications style
Li HL, Wu H, Zhang BB, Shi HL and Wu XJ: MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo. Mol Med Rep 14: 1430-1438, 2016.
APA
Li, H., Wu, H., Zhang, B., Shi, H., & Wu, X. (2016). MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo. Molecular Medicine Reports, 14, 1430-1438. https://doi.org/10.3892/mmr.2016.5361
MLA
Li, H., Wu, H., Zhang, B., Shi, H., Wu, X."MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo". Molecular Medicine Reports 14.2 (2016): 1430-1438.
Chicago
Li, H., Wu, H., Zhang, B., Shi, H., Wu, X."MAPK pathways are involved in the inhibitory effect of berberine hydrochloride on gastric cancer MGC 803 cell proliferation and IL-8 secretion in vitro and in vivo". Molecular Medicine Reports 14, no. 2 (2016): 1430-1438. https://doi.org/10.3892/mmr.2016.5361
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