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Article Open Access

Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis

  • Authors:
    • Zhao‑Xin Jiang
    • Suo Qiu
    • Bing‑Sheng Lou
    • Yao Yang
    • Wen‑Cong Wang
    • Xiao‑Feng Lin
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑Sen University, Guangzhou, Guangdong 510060, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1083-1090
    |
    Published online on: June 3, 2016
       https://doi.org/10.3892/mmr.2016.5362
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Abstract

Neutrophils have been recognized as critical response cells during the pathogenesis of endotoxin‑induced uveitis (EIU). Apoptosis of neutrophils induced by roscovitine has previously been demonstrated to ameliorate inflammation in several in vivo models. The present study aimed to assess whether roscovitine ameliorates EIU. EIU was induced in female C57BL/6 mice by a single intravitreal injection of lipopolysaccharide (LPS; 250 ng). The mice were divided into three groups as follows: LPS alone, LPS plus vehicle, LPS plus roscovitine (50 mg/kg). The mice were euthanized 12, 24, 48 and 72 h after LPS‑induced uveitis. Accumulation of inflammatory cells in the vitreous body was confirmed by immunohistochemistry, and quantified following hematoxylin and eosin staining. Terminal deoxynucleotidyl transferase dUTP nick‑end labeling was performed to detect of apoptotic cells. The mRNA levels of inflammatory cytokines were analyzed by reverse transcription‑quantitative polymerase chain reaction and the changes in protein levels were analyzed by western blotting. Inflammatory cells accumulated in the vitreous near the optic nerve head and the quantity peaked at 24 h after LPS injection. Immunohistochemistry revealed that the majority of the inflammatory cells were neutrophils. The number of infiltrating cells was similar in the LPS and LPS plus vehicle groups, while there were significantly less in the roscovitine group at 24 h. Apoptosis of neutrophils was observed between 12 and 48 h after roscovitine injection, while no apoptosis was observed in the other groups. The mRNA expression levels of GMCSF, CINC‑1 and ICAM‑1 peaked at 12 h after LPS injection, and decreased to normal levels at 72 h. This trend in mRNA expression was similar in the LPS and LPS plus vehicle groups; however, the expression levels decreased more quickly in the roscovitine group at 24 and 48 h. Following roscovitine administration, upregulated cleaved caspase 3 expression levels and downregulated Mcl‑1 expression levels were observed. In conclusion, roscovitine ameliorates EIU by effecting neutrophil apoptosis. Timely apoptosis of neutrophils may be an effective process to promote the amelioration of EIU.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang ZX, Qiu S, Lou BS, Yang Y, Wang WC and Lin XF: Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis. Mol Med Rep 14: 1083-1090, 2016.
APA
Jiang, Z., Qiu, S., Lou, B., Yang, Y., Wang, W., & Lin, X. (2016). Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis. Molecular Medicine Reports, 14, 1083-1090. https://doi.org/10.3892/mmr.2016.5362
MLA
Jiang, Z., Qiu, S., Lou, B., Yang, Y., Wang, W., Lin, X."Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis". Molecular Medicine Reports 14.2 (2016): 1083-1090.
Chicago
Jiang, Z., Qiu, S., Lou, B., Yang, Y., Wang, W., Lin, X."Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis". Molecular Medicine Reports 14, no. 2 (2016): 1083-1090. https://doi.org/10.3892/mmr.2016.5362
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang ZX, Qiu S, Lou BS, Yang Y, Wang WC and Lin XF: Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis. Mol Med Rep 14: 1083-1090, 2016.
APA
Jiang, Z., Qiu, S., Lou, B., Yang, Y., Wang, W., & Lin, X. (2016). Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis. Molecular Medicine Reports, 14, 1083-1090. https://doi.org/10.3892/mmr.2016.5362
MLA
Jiang, Z., Qiu, S., Lou, B., Yang, Y., Wang, W., Lin, X."Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis". Molecular Medicine Reports 14.2 (2016): 1083-1090.
Chicago
Jiang, Z., Qiu, S., Lou, B., Yang, Y., Wang, W., Lin, X."Roscovitine ameliorates endotoxin-induced uveitis through neutrophil apoptosis". Molecular Medicine Reports 14, no. 2 (2016): 1083-1090. https://doi.org/10.3892/mmr.2016.5362
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