Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation

Fu,Xiumei; Tong,Zhaoxue; Li,Qi; Niu,Qingfei; Zhang,Zhe; Tong,Xiaojie; Tong,Lei; Zhang,Xu

doi:10.3892/mmr.2016.5367

Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation

Authors:
- Xiumei Fu
- Zhaoxue Tong
- Qi Li
- Qingfei Niu
- Zhe Zhang
- Xiaojie Tong
- Lei Tong
- Xu Zhang
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Affiliations: Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Hand Surgery, Affiliated Feng Tian Hospital, Shenyang Medical College, Shenyang, Liaoning 110001, P.R. China
Published online on: June 6, 2016 https://doi.org/10.3892/mmr.2016.5367
Pages: 1187-1193
Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The peripheral nervous system has the potential for full regeneration following injury and recovery, predominantly controlled by Schwann cells (SCs). Therefore, obtaining a sufficient number of SCs in a short duration is crucial. In the present study, rat adipose‑derived stem cells (ADSCs) were isolated and cultured, following which characterization of the ADSCs was performed using flow cytometry. The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers. The multilineage differentiation potential of the ADSCs was assayed by determining the ability of the cells to differentiate into osteoblasts and adipocytes. Following this, the ADSCs were treated with a specific medium and differentiated into Schwann‑like cells. Immunofluorescence, western blot and reverse transcription‑quantitative polymerase chain reaction analyses showed that ~95% of the differentiated cells expressed glial fibrillary acidic protein, S100 and p75. In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells. These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

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