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N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells

  • Authors:
    • Yong Zhou
    • Shui‑Xiang Yang
    • Yu‑Nan Yue
    • Xiao‑Fei Wei
    • Yan Liu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, P.R. China, Emergency Department, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, P.R. China, Emergency Department, The 302 Hospital of People's Liberation Army, Beijing 100039, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1061-1066
    |
    Published online on: June 7, 2016
       https://doi.org/10.3892/mmr.2016.5372
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Abstract

The aim of the present study was to examine the effect of small interfering RNA (siRNA) methods on the expression of N‑ethylmaleimide sensitive factor (NSF) and Weibel‑Palade body (WPB) release in endothelial cells. A small hairpin RNA (shRNA), mediated with an adenovirus vector, was designed to target the N‑terminal functional area of NSF. Subsequently, viruses were transfected into human aortic endothelial cells. The mRNA and protein expression levels of NSF were detected using reverse transcription‑quantitative polymerase chain reaction and Western blot analyses, respectively, and the release of WPBs in the endothelial cells was examined using immunofluorescence. The mRNA expression of NSF in the endothelial cells, which were transfected with the adenoviruses carrying the NSF‑shRNA was significantly decreased, compared with the negative control group (P=0.035) and blank control group (P=0.02). In addition, the mRNA expression of NSF was gradually decreased as duration increased; there were marked differences between the 24, 48 and 72 h groups (P<0.05). The protein expression of NSF was significantly decreased in the experimental group, compared with the negative control group (P=0.004) and blank control group (P=0.031), however, no difference was observed between the negative control and blank control groups (P=0.249). The immunofluorescence staining showed that the release of WPBs in the endothelial cells induced with thrombin was inhibited markedly following transfection with the virus carrying the NSF‑shRNA. Therefore NSF‑siRNA inhibited the mRNA and protein expression levels of NSF, and inhibited the release of WPBs in endothelial cells induced with thrombin. These results suggested that NSF-siRNA may be valuable for preventing and treating atherosclerosis and acute coronary syndrome.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Yang SX, Yue YN, Wei XF and Liu Y: N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells. Mol Med Rep 14: 1061-1066, 2016.
APA
Zhou, Y., Yang, S., Yue, Y., Wei, X., & Liu, Y. (2016). N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells. Molecular Medicine Reports, 14, 1061-1066. https://doi.org/10.3892/mmr.2016.5372
MLA
Zhou, Y., Yang, S., Yue, Y., Wei, X., Liu, Y."N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells". Molecular Medicine Reports 14.2 (2016): 1061-1066.
Chicago
Zhou, Y., Yang, S., Yue, Y., Wei, X., Liu, Y."N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells". Molecular Medicine Reports 14, no. 2 (2016): 1061-1066. https://doi.org/10.3892/mmr.2016.5372
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Yang SX, Yue YN, Wei XF and Liu Y: N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells. Mol Med Rep 14: 1061-1066, 2016.
APA
Zhou, Y., Yang, S., Yue, Y., Wei, X., & Liu, Y. (2016). N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells. Molecular Medicine Reports, 14, 1061-1066. https://doi.org/10.3892/mmr.2016.5372
MLA
Zhou, Y., Yang, S., Yue, Y., Wei, X., Liu, Y."N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells". Molecular Medicine Reports 14.2 (2016): 1061-1066.
Chicago
Zhou, Y., Yang, S., Yue, Y., Wei, X., Liu, Y."N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells". Molecular Medicine Reports 14, no. 2 (2016): 1061-1066. https://doi.org/10.3892/mmr.2016.5372
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