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Article

Human GPR4 and the Notch signaling pathway in endothelial cell tube formation

  • Authors:
    • Juan Ren
    • Yuelang Zhang
    • Hui Cai
    • Hongbing Ma
    • Dongli Zhao
    • Xiaozhi Zhang
    • Zongfang Li
    • Shufeng Wang
    • Jiangsheng Wang
    • Rui Liu
    • Yi Li
    • Jiansheng Qian
    • Hongxia Wei
    • Liying Niu
    • Yan Liu
    • Lisha Xiao
    • Muyang Ding
    • Shiwen Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Radiotherapy, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Imaging Department, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Medical School, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Oncology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Biomedical Science, Mercer University School of Medicine, Macon, GA 31207, USA
  • Pages: 1235-1240
    |
    Published online on: June 9, 2016
       https://doi.org/10.3892/mmr.2016.5380
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Abstract

G protein-coupled receptor 4 (GPR4) is hypothesized to function as a pH sensor and is important in the regulation of proliferation, migration and angiogenesis of vascular endothelial cells (ECs). Furthermore, the Notch signaling pathway is significant in the regulation of the angiogenic behavior of ECs. However, whether GPR4 regulates angiogenesis via the Notch signaling pathway remains unclear. The present study evaluated the effect of Notch signaling in human GPR4‑induced angiogenesis in HMEC‑1 cells. The results revealed that GPR4 increased Notch1 expression in a time‑dependent manner. In addition, the inhibition of Notch1 expression using small interfering RNA or the Notch receptor inhibitor, γ-secretase inhibitor I, significantly blocked GPR4‑induced HMEC‑1 tube formation and lymphocyte transendothelial migration. Furthermore, the inhibition of Notch1 blocked GPR4‑induced vascular endothelial growth factor and hypoxia-inducible factor 1α expression. Thus, it was demonstrated that GPR4 affects ECs by regulating Notch1, a function that may be important for physiological and pathological angiogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Ren J, Zhang Y, Cai H, Ma H, Zhao D, Zhang X, Li Z, Wang S, Wang J, Liu R, Liu R, et al: Human GPR4 and the Notch signaling pathway in endothelial cell tube formation. Mol Med Rep 14: 1235-1240, 2016.
APA
Ren, J., Zhang, Y., Cai, H., Ma, H., Zhao, D., Zhang, X. ... Jiang, S. (2016). Human GPR4 and the Notch signaling pathway in endothelial cell tube formation. Molecular Medicine Reports, 14, 1235-1240. https://doi.org/10.3892/mmr.2016.5380
MLA
Ren, J., Zhang, Y., Cai, H., Ma, H., Zhao, D., Zhang, X., Li, Z., Wang, S., Wang, J., Liu, R., Li, Y., Qian, J., Wei, H., Niu, L., Liu, Y., Xiao, L., Ding, M., Jiang, S."Human GPR4 and the Notch signaling pathway in endothelial cell tube formation". Molecular Medicine Reports 14.2 (2016): 1235-1240.
Chicago
Ren, J., Zhang, Y., Cai, H., Ma, H., Zhao, D., Zhang, X., Li, Z., Wang, S., Wang, J., Liu, R., Li, Y., Qian, J., Wei, H., Niu, L., Liu, Y., Xiao, L., Ding, M., Jiang, S."Human GPR4 and the Notch signaling pathway in endothelial cell tube formation". Molecular Medicine Reports 14, no. 2 (2016): 1235-1240. https://doi.org/10.3892/mmr.2016.5380
Copy and paste a formatted citation
x
Spandidos Publications style
Ren J, Zhang Y, Cai H, Ma H, Zhao D, Zhang X, Li Z, Wang S, Wang J, Liu R, Liu R, et al: Human GPR4 and the Notch signaling pathway in endothelial cell tube formation. Mol Med Rep 14: 1235-1240, 2016.
APA
Ren, J., Zhang, Y., Cai, H., Ma, H., Zhao, D., Zhang, X. ... Jiang, S. (2016). Human GPR4 and the Notch signaling pathway in endothelial cell tube formation. Molecular Medicine Reports, 14, 1235-1240. https://doi.org/10.3892/mmr.2016.5380
MLA
Ren, J., Zhang, Y., Cai, H., Ma, H., Zhao, D., Zhang, X., Li, Z., Wang, S., Wang, J., Liu, R., Li, Y., Qian, J., Wei, H., Niu, L., Liu, Y., Xiao, L., Ding, M., Jiang, S."Human GPR4 and the Notch signaling pathway in endothelial cell tube formation". Molecular Medicine Reports 14.2 (2016): 1235-1240.
Chicago
Ren, J., Zhang, Y., Cai, H., Ma, H., Zhao, D., Zhang, X., Li, Z., Wang, S., Wang, J., Liu, R., Li, Y., Qian, J., Wei, H., Niu, L., Liu, Y., Xiao, L., Ding, M., Jiang, S."Human GPR4 and the Notch signaling pathway in endothelial cell tube formation". Molecular Medicine Reports 14, no. 2 (2016): 1235-1240. https://doi.org/10.3892/mmr.2016.5380
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