Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2016 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2016 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Methylation status of the promoter region of the human frizzled 9 gene in acute myeloid leukemia

  • Authors:
    • Yingjie Zhang
    • Qi Jiang
    • Xiaolin Kong
    • Lili Yang
    • Wanzhen Hu
    • Chengfang Lv
    • Yinghua Li
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 1339-1344
    |
    Published online on: June 10, 2016
       https://doi.org/10.3892/mmr.2016.5387
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The FZD9 gene is located at chromosome 7q11.23, and has been indicated to be a tumor suppressor gene. The present study examined the involvement of FZD9 promoter methylation in the downregulation of FZD9 expression in leukemia cells. The expression of the FZD9 gene was absent in various leukemic cell lines, while it was restored following treatment with DNA demethylating agent 5‑aza‑2'‑deoxycytidine. Bisulfite sequencing analysis of the FZD9 promoter region showed that it was partially methylated in cell lines in which FZD9 gene was not expressed. Thus, DNA methylation in the promoter region may lead to inactivation of the FZD9 gene, which may represent and aberration associated with leukemia, since DNA was not methylated in normal peripheral blood mononuclear cells. Methylation‑specific polymerase chain reaction analysis revealed that the promoter region of the FZD9 gene was frequently methylated in primary or relapse acute myeloid leukemia (52.9%; excluding acute promyelocytic leukemia); however, methylation was infrequent in B‑cell acute lymphocytic leukemia (5.6%). In conclusion, the present study indicated that the methylation profile of the FZD9 gene corresponded to that of a candidate tumor‑suppressor gene in acute myeloid leukemia.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Löwenberg B, Downing JR and Burnett A: Acute myeloid leukemia. N Engl J Med. 341:1051–1062. 1999. View Article : Google Scholar : PubMed/NCBI

2 

Jones PA and Baylin SB: The fundamental role of epigenetic events in cancer. Nat Rev Genet. 3:415–428. 2002.PubMed/NCBI

3 

Kaucká M, Plevová K, Pavlová S, Janovská P, Mishra A, Verner J, Procházková J, Krejcí P, Kotasková J, Ovesná P, et al: The planar cell polarity pathway drives pathogenesis of chronic lymphocytic leukemia by the regulation of B-lymphocyte migration. Cancer Res. 73:1491–1501. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Huelsken J, Vogel R, Brinkmann V, Erdmann B, Birchmeier C and Birchmeier W: Requirement for beta-catenin in anterior-posterior axis formation in mice. J Cell Biol. 148:567–578. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Giles RH, van Es JH and Clevers H: Caught up in a Wnt storm: Wnt signaling in cancer. Biochim Biophys Acta. 1653:1–24. 2003.PubMed/NCBI

6 

Yu S, Zhou X, Steinke FC, Liu C, Chen SC, Zagorodna O, Jing X, Yokota Y, Meyerholz DK, Mullighan CG, et al: The TCF-1 and LEF-1 transcription factors have cooperative and opposing roles in T cell development and malignancy. Immunity. 37:813–826. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Xu Y, Banerjee D, Huelsken J, Birchmeier W and Sen JM: Deletion of beta-catenin impairs T cell development. Nat Immunol. 4:1177–1182. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Fusco C, Micale L, Augello B, Teresa Pellico M, Menghini D, Alfieri P, Cristina Digilio M, Mandriani B, Carella M, Palumbo O, et al: Smaller and larger deletions of the Williams Beuren syndrome region implicate genes involved in mild facial phenotype, epilepsy and autistic traits. Eur J Hum Genet. 22:64–70. 2014. View Article : Google Scholar

9 

Laurito S, Branham T, Herrero G, Marsa S, Garro F and Roqué M: Detection of a Williams Beuren syndrome case by MLPA. Medicina (B Aires). 73:47–50. 2013.In Spanish.

10 

Van Hagen JM, Eussen HJ, Van Schooten R, van Der Geest JN, Lagers-van Haselen GC, Wouters CH, De Zeeuw CI and Gille JJ: Comparing two diagnostic laboratory tests for Williams syndrome: Fluorescent in situ hybridization versus multiplex ligation-dependent probe amplification. Genet Test. 11:321–327. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Kolben T, Peröbner I, Fernsebner K, Lechner F, Geissler C, Ruiz-Heinrich L, Capovilla S, Jochum M and Neth P: Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells. Biol Chem. 393:1433–1447. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz L, Nusse R and Weissman IL: A role for Wnt signalling in self-renewal of haematopoietic stem cells. Nature. 423:409–414. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Reya T, Morrison SJ, Clarke MF and Weissman IL: Stem cells, cancer and cancer stem cells. Nature. 414:105–111. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Huelsken J, Vogel R, Erdmann B, Cotsarelis G and Birchmeier W: beta-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell. 105:533–545. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Ranheim EA, Kwan HC, Reya T, Wang YK, Weissman IL and Francke U: Frizzled 9 knock-out mice have abnormal B-cell development. Blood. 105:2487–2494. 2005. View Article : Google Scholar

16 

Jiang Y, Dunbar A, Gondek LP, Mohan S, Rataul M, O'Keefe C, Sekeres M, Saunthararajah Y and Maciejewski JP: Aberrant DNA methylation is a dominant mechanism in MDS progression to AML. Blood. 113:1315–1325. 2009. View Article : Google Scholar :

17 

Tennis M, Van Scoyk M, Heasley LE, Vandervest K, Weiser-Evans M, Freeman S, Keith RL, Simpson P, Nemenoff RA and Winn RA: Prostacyclin Inhibits non-small cell lung cancer growth by a frizzled 9-dependent pathway that is blocked by secreted frizzled-related protein 1. Neoplasia. 12:244–253. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Winn RA, Van Scoyk M, Hammond M, Rodriguez K, Crossno JT Jr, Heasley LE and Nemenoff RA: Antitumorigenic effect of Wnt 7a and Fzd 9 in non-small cell lung cancer cells is mediated through ERK-5-dependent activation of peroxisome proliferator-activated receptor gamma. J Biol Chem. 281:26943–26950. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Khan NI and Bendall LJ: Role of WNT signaling in normal and malignant hematopoiesis. Histol Histopathol. 21:761–774. 2006.PubMed/NCBI

20 

Román-Gómez J, Cordeu L, Agirre X, Jiménez-Velasco A, San José-Eneriz E, Garate L, Calasanz MJ, Heiniger A, Torres A and Prosper F: Epigenetic regulation of Wnt-signaling pathway in acute lymphoblastic leukemia. Blood. 109:3462–3469. 2007. View Article : Google Scholar

21 

Liu TH, Raval A, Chen SS, Matkovic JJ, Byrd JC and Plass C: CpG island methylation and expression of the secreted frizzled-related protein gene family in chronic lymphocytic leukemia. Cancer Res. 66:653–658. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Borssén M, Palmqvist L, Karrman K, Abrahamsson J, Behrendtz M, Heldrup J, Forestier E, Roos G and Degerman S: Promoter DNA methylation pattern identifies prognostic subgroups in childhood T-cell acute lymphoblastic leukemia. PLoS One. 8:e653732013. View Article : Google Scholar : PubMed/NCBI

23 

Zhang Y, Li Q and Chen H: DNA methylation and histone modifications of Wnt genes by genistein during colon cancer development. Carcinogenesis. 34:1756–1763. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Portela A, Liz J, Nogales V, Setién F, Villanueva A and Esteller M: DNA methylation determines nucleosome occupancy in the 5′-CpG islands of tumor suppressor genes. Oncogene. 32:5421–5428. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Wodarz D, Boland CR, Goel A and Komarova NL: Methylation kinetics and CpG-island methylator phenotyope status in colorectal cancer cell lines. Biol Direct. 8:142013. View Article : Google Scholar :

26 

He W, Li X, Xu S, Ai J, Gong Y, Gregg JL, Guan R, Qiu W, Xin D, Gingrich JR, et al: Aberrant methylation and loss of CADM2 tumor suppressor expression is associated with human renal cell carcinoma tumor progression. Biochem Biophys Res Commun. 435:526–532. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Twelves D, Nerurkar A, Osin P, Dexter T, Ward A, Gui GP and Isacke CM: DNA promoter hypermethylation profiles in breast duct fluid. Breast Cancer Res Treat. 139:341–350. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Zhang L, McHale CM, Rothman N, Li G, Ji Z, Vermeulen R, Hubbard AE, Ren X, Shen M, Rappaport SM, et al: Systems biology of human benzene exposure. Chem Biol Interact. 184:86–93. 2010. View Article : Google Scholar :

29 

Lin X, Wang Z, Wang Y and Feng W: Serum MicroRNA-370 as a potential diagnostic and prognostic biomarker for pediatric acute myeloid leukemia. Int J Clin Exp Pathol. 8:14658–14666. 2015.

30 

Koon HW, Shih DQ, Hing TC, Yoo JH, Ho S, Chen X, Kelly CP, Targan SR and Pothoulakis C: Human monoclonal antibodies against Clostridium difficile toxins A and B inhibit inflammatory and histologic responses to the toxins in human colon and peripheral blood monocytes. Antimicrob Agents Chemother. 57:3214–3223. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Xia L, Gong Y, Zhang A, Cai S and Zeng Q: Loss of GATA5 expression due to gene promoter methylation induces growth and colony formation of hepatocellular carcinoma cells. Oncol Lett. 11:861–869. 2016.PubMed/NCBI

32 

Li Y, Nagai H, Ohno T, Yuge M, Hatano S, Ito E, Mori N, Saito H and Kinoshita T: Aberrant DNA methylation of p57(KIP2) gene in the promoter region in lymphoid malignancies of B-cell phenotype. Blood. 100:2572–2577. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Uchida T, Kinoshita T, Ohno T, Ohashi H, Nagai H and Saito H: Hypermethylation of p16INK4A gene promoter during the progression of plasma cell dyscrasia. Leukemia. 15:157–165. 2001. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Jiang Q, Kong X, Yang L, Hu W, Lv C and Li Y: Methylation status of the promoter region of the human frizzled 9 gene in acute myeloid leukemia. Mol Med Rep 14: 1339-1344, 2016.
APA
Zhang, Y., Jiang, Q., Kong, X., Yang, L., Hu, W., Lv, C., & Li, Y. (2016). Methylation status of the promoter region of the human frizzled 9 gene in acute myeloid leukemia. Molecular Medicine Reports, 14, 1339-1344. https://doi.org/10.3892/mmr.2016.5387
MLA
Zhang, Y., Jiang, Q., Kong, X., Yang, L., Hu, W., Lv, C., Li, Y."Methylation status of the promoter region of the human frizzled 9 gene in acute myeloid leukemia". Molecular Medicine Reports 14.2 (2016): 1339-1344.
Chicago
Zhang, Y., Jiang, Q., Kong, X., Yang, L., Hu, W., Lv, C., Li, Y."Methylation status of the promoter region of the human frizzled 9 gene in acute myeloid leukemia". Molecular Medicine Reports 14, no. 2 (2016): 1339-1344. https://doi.org/10.3892/mmr.2016.5387
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Jiang Q, Kong X, Yang L, Hu W, Lv C and Li Y: Methylation status of the promoter region of the human frizzled 9 gene in acute myeloid leukemia. Mol Med Rep 14: 1339-1344, 2016.
APA
Zhang, Y., Jiang, Q., Kong, X., Yang, L., Hu, W., Lv, C., & Li, Y. (2016). Methylation status of the promoter region of the human frizzled 9 gene in acute myeloid leukemia. Molecular Medicine Reports, 14, 1339-1344. https://doi.org/10.3892/mmr.2016.5387
MLA
Zhang, Y., Jiang, Q., Kong, X., Yang, L., Hu, W., Lv, C., Li, Y."Methylation status of the promoter region of the human frizzled 9 gene in acute myeloid leukemia". Molecular Medicine Reports 14.2 (2016): 1339-1344.
Chicago
Zhang, Y., Jiang, Q., Kong, X., Yang, L., Hu, W., Lv, C., Li, Y."Methylation status of the promoter region of the human frizzled 9 gene in acute myeloid leukemia". Molecular Medicine Reports 14, no. 2 (2016): 1339-1344. https://doi.org/10.3892/mmr.2016.5387
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team