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Article Open Access

Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway

  • Authors:
    • Lin Cui
    • Youping Wang
    • Rui Yu
    • Bin Li
    • Shiyang Xie
    • Yuan Gao
    • Xiaoxiao Wang
    • Mingjun Zhu
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450000, P.R. China, Department of Internal Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450000, P.R. China, Division of Cardiology, First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450000, P.R. China
    Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1610-1616
    |
    Published online on: June 16, 2016
       https://doi.org/10.3892/mmr.2016.5405
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Abstract

Jia-Shen decoction (JSD) is a traditional Chinese medicine, which is used widely to treat chronic heart failure. However, the underlying mechanism remains to be elucidated. The present study aimed to investigate the mechanism underlying the effects of JSD on cardiac fibroblast (CF) proliferation and differentiation. The CFs were obtained from the hearts of neonatal (1‑3‑day old) Sprague‑Dawley rats and treated with JSD-medicated serum (JSDS) with or without angiotensin II (Ang II). Cell proliferation was assessed using Cell Counting Kit‑8 reagent. In addition, the mRNA expression levels of transforming growth factor‑β1 (TGF‑β1) and phosphorylated small mothers against decapentaplegic (p‑Smad)2/3 and their protein expression levels were analyzed. CF proliferation was significantly increased in the Ang II‑treated group, compared with the control group (P<0.05). The expression levels of collagen, α‑smooth muscle actin, TGF‑β1 and p‑Smad2/3 were also increased in the Ang II‑treated group (P<0.05). Following JSDS treatment, the increased levels of collagen and cell proliferation were inhibited, and the increased expression levels of p‑Smad2 and p‑Smad3 were also inhibited (P<0.05). These data suggested that JSDS may inhibit CF proliferation via attenuating the TGF‑β1/Smad signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Cui L, Wang Y, Yu R, Li B, Xie S, Gao Y, Wang X and Zhu M: Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway. Mol Med Rep 14: 1610-1616, 2016.
APA
Cui, L., Wang, Y., Yu, R., Li, B., Xie, S., Gao, Y. ... Zhu, M. (2016). Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway. Molecular Medicine Reports, 14, 1610-1616. https://doi.org/10.3892/mmr.2016.5405
MLA
Cui, L., Wang, Y., Yu, R., Li, B., Xie, S., Gao, Y., Wang, X., Zhu, M."Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway". Molecular Medicine Reports 14.2 (2016): 1610-1616.
Chicago
Cui, L., Wang, Y., Yu, R., Li, B., Xie, S., Gao, Y., Wang, X., Zhu, M."Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway". Molecular Medicine Reports 14, no. 2 (2016): 1610-1616. https://doi.org/10.3892/mmr.2016.5405
Copy and paste a formatted citation
x
Spandidos Publications style
Cui L, Wang Y, Yu R, Li B, Xie S, Gao Y, Wang X and Zhu M: Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway. Mol Med Rep 14: 1610-1616, 2016.
APA
Cui, L., Wang, Y., Yu, R., Li, B., Xie, S., Gao, Y. ... Zhu, M. (2016). Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway. Molecular Medicine Reports, 14, 1610-1616. https://doi.org/10.3892/mmr.2016.5405
MLA
Cui, L., Wang, Y., Yu, R., Li, B., Xie, S., Gao, Y., Wang, X., Zhu, M."Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway". Molecular Medicine Reports 14.2 (2016): 1610-1616.
Chicago
Cui, L., Wang, Y., Yu, R., Li, B., Xie, S., Gao, Y., Wang, X., Zhu, M."Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-β1/Smad signaling pathway". Molecular Medicine Reports 14, no. 2 (2016): 1610-1616. https://doi.org/10.3892/mmr.2016.5405
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