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Article

Role of GLTSCR2 in the regulation of telomerase activity and chromosome stability

  • Authors:
    • Jee‑Youn Kim
    • Yong‑Min An
    • Jae‑Hoon Park
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, School of Medicine, Kyung Hee University, Seoul 130‑701, Republic of Korea
  • Pages: 1697-1703
    |
    Published online on: June 23, 2016
       https://doi.org/10.3892/mmr.2016.5427
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Abstract

Telomerase is essential for regulating telomeres, and its activation is a critical step in cellular immortalization and tumorigenesis. The transcriptional activation of human telomerase reverse transcriptase (hTERT) is critical for telomerase expression. Although several transcriptional activators have been identified, factors responsible for enhancing the hTERT promoter remain to be fully elucidated. In the present study, the role of glioma tumor-suppressor candidate region gene 2 (GLTSCR2) in telomerase regulation was analyzed. A doxycyclin-inducible green fluorescent protein (GFP)-tagged GLTSCR2-expressing adenovirus (Ad‑GLT/GFP) was used for the transduction of SK‑Hep‑1 and T98G cancer cells, and normal human umbilical vein endothelial cells. Changes in telomerase activity using telomere repeat amplification protocol assay were assessed, and the gene expression levels of hTERT were then examined. To investigate chromosome instability and senescence, Giemsa and β-galactosidase staining was performed. The results revealed that overexpression of GLTSCR2 significantly increased telomerase activity in the cancer and normal cell lines. This increase was consistent with increases in the protein and mRNA expression levels of hTERT. In luciferase assays, the hTERT promoter was activated by GLTSCR2. Knockdown of GLTSCR2 led to the downregulation of telomerase activity, abnormal nuclear morphology as a marker of chromosome instability, significant suppression of growth rate, alterations in cellular morphology and, eventually, cellular senescence. Taken together, the results of the present study suggested that GLTSCR2 is crucially involved in the positive regulation of telomerase and chromosome stability.
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Copy and paste a formatted citation
Spandidos Publications style
Kim JY, An YM and Park JH: Role of GLTSCR2 in the regulation of telomerase activity and chromosome stability. Mol Med Rep 14: 1697-1703, 2016.
APA
Kim, J., An, Y., & Park, J. (2016). Role of GLTSCR2 in the regulation of telomerase activity and chromosome stability. Molecular Medicine Reports, 14, 1697-1703. https://doi.org/10.3892/mmr.2016.5427
MLA
Kim, J., An, Y., Park, J."Role of GLTSCR2 in the regulation of telomerase activity and chromosome stability". Molecular Medicine Reports 14.2 (2016): 1697-1703.
Chicago
Kim, J., An, Y., Park, J."Role of GLTSCR2 in the regulation of telomerase activity and chromosome stability". Molecular Medicine Reports 14, no. 2 (2016): 1697-1703. https://doi.org/10.3892/mmr.2016.5427
Copy and paste a formatted citation
x
Spandidos Publications style
Kim JY, An YM and Park JH: Role of GLTSCR2 in the regulation of telomerase activity and chromosome stability. Mol Med Rep 14: 1697-1703, 2016.
APA
Kim, J., An, Y., & Park, J. (2016). Role of GLTSCR2 in the regulation of telomerase activity and chromosome stability. Molecular Medicine Reports, 14, 1697-1703. https://doi.org/10.3892/mmr.2016.5427
MLA
Kim, J., An, Y., Park, J."Role of GLTSCR2 in the regulation of telomerase activity and chromosome stability". Molecular Medicine Reports 14.2 (2016): 1697-1703.
Chicago
Kim, J., An, Y., Park, J."Role of GLTSCR2 in the regulation of telomerase activity and chromosome stability". Molecular Medicine Reports 14, no. 2 (2016): 1697-1703. https://doi.org/10.3892/mmr.2016.5427
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