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CMP‑N‑acetylneuraminic acid synthetase interacts with fragile X related protein 1

  • Authors:
    • Yun Ma
    • Shuai Tian
    • Zongbao Wang
    • Changbo Wang
    • Xiaowei Chen
    • Wei Li
    • Yang Yang
    • Shuya He
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry & Biology, University of South China, Hengyang, Hunan 421001, P.R. China, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmaceutical and Biological Sciences, University of South China, Hengyang, Hunan 421001, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1501-1508
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    Published online on: June 23, 2016
       https://doi.org/10.3892/mmr.2016.5438
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Abstract

Fragile X mental retardation protein (FMRP), fragile X related 1 protein (FXR1P) and FXR2P are the members of the FMR protein family. These proteins contain two KH domains and a RGG box, which are characteristic of RNA binding proteins. The absence of FMRP, causes fragile X syndrome (FXS), the leading cause of hereditary mental retardation. FXR1P is expressed throughout the body and important for normal muscle development, and its absence causes cardiac abnormality. To investigate the functions of FXR1P, a screen was performed to identify FXR1P‑interacting proteins and determine the biological effect of the interaction. The current study identified CMP‑N‑acetylneuraminic acid synthetase (CMAS) as an interacting protein using the yeast two‑hybrid system, and the interaction between FXR1P and CMAS was validated in yeast using a β‑galactosidase assay and growth studies with selective media. Furthermore, co‑immunoprecipitation was used to analyze the FXR1P/CMAS association and immunofluorescence microscopy was performed to detect expression and intracellular localization of the proteins. The results of the current study indicated that FXR1P and CMAS interact, and colocalize in the cytoplasm and the nucleus of HEK293T and HeLa cells. Accordingly, a fragile X related 1 (FXR1) gene overexpression vector was constructed to investigate the effect of FXR1 overexpression on the level of monosialotetrahexosylganglioside 1 (GM1). The results of the current study suggested that FXR1P is a tissue‑specific regulator of GM1 levels in SH‑SY5Y cells, but not in HEK293T cells. Taken together, the results initially indicate that FXR1P interacts with CMAS, and that FXR1P may enhance the activation of sialic acid via interaction with CMAS, and increase GM1 levels to affect the development of the nervous system, thus providing evidence for further research into the pathogenesis of FXS.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Tian S, Wang Z, Wang C, Chen X, Li W, Yang Y and He S: CMP‑N‑acetylneuraminic acid synthetase interacts with fragile X related protein 1. Mol Med Rep 14: 1501-1508, 2016.
APA
Ma, Y., Tian, S., Wang, Z., Wang, C., Chen, X., Li, W. ... He, S. (2016). CMP‑N‑acetylneuraminic acid synthetase interacts with fragile X related protein 1. Molecular Medicine Reports, 14, 1501-1508. https://doi.org/10.3892/mmr.2016.5438
MLA
Ma, Y., Tian, S., Wang, Z., Wang, C., Chen, X., Li, W., Yang, Y., He, S."CMP‑N‑acetylneuraminic acid synthetase interacts with fragile X related protein 1". Molecular Medicine Reports 14.2 (2016): 1501-1508.
Chicago
Ma, Y., Tian, S., Wang, Z., Wang, C., Chen, X., Li, W., Yang, Y., He, S."CMP‑N‑acetylneuraminic acid synthetase interacts with fragile X related protein 1". Molecular Medicine Reports 14, no. 2 (2016): 1501-1508. https://doi.org/10.3892/mmr.2016.5438
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Tian S, Wang Z, Wang C, Chen X, Li W, Yang Y and He S: CMP‑N‑acetylneuraminic acid synthetase interacts with fragile X related protein 1. Mol Med Rep 14: 1501-1508, 2016.
APA
Ma, Y., Tian, S., Wang, Z., Wang, C., Chen, X., Li, W. ... He, S. (2016). CMP‑N‑acetylneuraminic acid synthetase interacts with fragile X related protein 1. Molecular Medicine Reports, 14, 1501-1508. https://doi.org/10.3892/mmr.2016.5438
MLA
Ma, Y., Tian, S., Wang, Z., Wang, C., Chen, X., Li, W., Yang, Y., He, S."CMP‑N‑acetylneuraminic acid synthetase interacts with fragile X related protein 1". Molecular Medicine Reports 14.2 (2016): 1501-1508.
Chicago
Ma, Y., Tian, S., Wang, Z., Wang, C., Chen, X., Li, W., Yang, Y., He, S."CMP‑N‑acetylneuraminic acid synthetase interacts with fragile X related protein 1". Molecular Medicine Reports 14, no. 2 (2016): 1501-1508. https://doi.org/10.3892/mmr.2016.5438
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