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Article

Ipr1 modified BCG as a novel vaccine induces stronger immunity than BCG against tuberculosis infection in mice

  • Authors:
    • Yuwei Wang
    • Chun Yang
    • Yonglin He
    • Xingxing Zhan
    • Lei Xu
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, P.R. China, Department of Microbiology, Chongqing Medical University, Chongqing 400016, P.R. China, Department of Pediatrics, Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 1756-1764
    |
    Published online on: June 27, 2016
       https://doi.org/10.3892/mmr.2016.5447
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Abstract

Tuberculosis is a major challenge to global public health. However, the Bacille Calmette‑Guérin (BCG), the only vaccine available against tuberculosis, has been questioned for the low protective effect. The present study used the mouse gene intracellular pathogen resistance I (Ipr1) gene to alter the current BCG vaccine and evaluated its immunity effect against tuberculosis. This study also investigated the intrinsic relationships of Ipr1 and innate immunity. The reformed BCG (BCGi) carrying the Ipr1 gene was constructed. The mice were intranasally challenged with the M. tuberculosis H37Rv strain after vaccination with BCGi. Protection efficacy of the vaccine was assessed by the organ coefficient, bacterial load and pathological changes in the lung. The differential expression of 113 immune‑related genes between BCGi and BCG groups were detected by an oligo microarray. According to the results of organ coefficient, bacterial load and pathological changes in the organization, BCGi had been shown to have stronger protective effects against M. tuberculosis than BCG. The oligo microarray and reverse transcription‑quantitative polymerase chain reaction further revealed that the Ipr1 gene could upregulate the expression of 13 genes, including a >3‑fold increase in Toll‑like receptor (TLR)4 and 10‑fold increase in surfactant protein D (sftpd). The two genes not only participate in innate immunity against pathogens, but also are closely interrelated. Ipr1 could activate the TLR4 and sftpd signaling pathway and improve the innate immunity against tuberculosis, therefore Ipr1 modified BCG may be a candidate vaccine against M. tuberculosis.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Yang C, He Y, Zhan X and Xu L: Ipr1 modified BCG as a novel vaccine induces stronger immunity than BCG against tuberculosis infection in mice. Mol Med Rep 14: 1756-1764, 2016.
APA
Wang, Y., Yang, C., He, Y., Zhan, X., & Xu, L. (2016). Ipr1 modified BCG as a novel vaccine induces stronger immunity than BCG against tuberculosis infection in mice. Molecular Medicine Reports, 14, 1756-1764. https://doi.org/10.3892/mmr.2016.5447
MLA
Wang, Y., Yang, C., He, Y., Zhan, X., Xu, L."Ipr1 modified BCG as a novel vaccine induces stronger immunity than BCG against tuberculosis infection in mice". Molecular Medicine Reports 14.2 (2016): 1756-1764.
Chicago
Wang, Y., Yang, C., He, Y., Zhan, X., Xu, L."Ipr1 modified BCG as a novel vaccine induces stronger immunity than BCG against tuberculosis infection in mice". Molecular Medicine Reports 14, no. 2 (2016): 1756-1764. https://doi.org/10.3892/mmr.2016.5447
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Yang C, He Y, Zhan X and Xu L: Ipr1 modified BCG as a novel vaccine induces stronger immunity than BCG against tuberculosis infection in mice. Mol Med Rep 14: 1756-1764, 2016.
APA
Wang, Y., Yang, C., He, Y., Zhan, X., & Xu, L. (2016). Ipr1 modified BCG as a novel vaccine induces stronger immunity than BCG against tuberculosis infection in mice. Molecular Medicine Reports, 14, 1756-1764. https://doi.org/10.3892/mmr.2016.5447
MLA
Wang, Y., Yang, C., He, Y., Zhan, X., Xu, L."Ipr1 modified BCG as a novel vaccine induces stronger immunity than BCG against tuberculosis infection in mice". Molecular Medicine Reports 14.2 (2016): 1756-1764.
Chicago
Wang, Y., Yang, C., He, Y., Zhan, X., Xu, L."Ipr1 modified BCG as a novel vaccine induces stronger immunity than BCG against tuberculosis infection in mice". Molecular Medicine Reports 14, no. 2 (2016): 1756-1764. https://doi.org/10.3892/mmr.2016.5447
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