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Article

Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells

  • Authors:
    • Esra Arslan Aydemir
    • Ece Şimşek
    • Aylin Fidan Korcum
    • Kayahan Fişkin
  • View Affiliations / Copyright

    Affiliations: Department of Biology, Science Faculty, Akdeniz University, Antalya 07058, Turkey, Department of Nutrition and Dietetics, Antalya School of Health, Akdeniz University, Antalya 07058, Turkey, Department of Radiation Oncology, School of Medicine, Akdeniz University, Antalya 07058, Turkey
  • Pages: 2343-2351
    |
    Published online on: July 1, 2016
       https://doi.org/10.3892/mmr.2016.5463
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Abstract

Angiogenesis, the formation of new blood vessels, is regarded as a key cancer cell property. Endostatin (ES) is a potential antiangiogenic agent and it may be useful when implemented in combination with other cancer therapeutic strategies. The present study investigated the in vitro effects of ES, radiotherapy (RT) or combination therapy (ES + RT) on two important proteases, a disintegrin and metalloproteinase domain‑containing protein 10 (ADAM10) and neprilysin (NEP) in 4T1 mouse breast cancer cells and the more metastatic phenotype of 4THMpc breast cancer cells. 4T1 and 4THMpc cells were treated with recombinant murine ES (4 µg/ml) alone, RT (45 Gy) alone or with ES + RT. ADAM10 enzyme activity was determined using a tumor necrosis factor‑α converting enzyme (α‑secretase) activity assay kit, and NEP enzyme activity was measured with a fluorometric assay based on the generation of free dansyl‑D‑Ala‑Gly from N-dansyl-Ala-Gly-D-nitro-Phe-Gly, the substrate of NEP. Western blotting analysis was performed to determine whether the altered enzyme activity levels of the two cell lines occurred due to changes in expression level. These data indicate that ES independently potentiates the activity of ADAM10 and NEP enzymes in 4T1 and 4THMpc breast cancer cells.
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Aydemir EA, Şimşek E, Korcum AF and Fişkin K: Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells. Mol Med Rep 14: 2343-2351, 2016.
APA
Aydemir, E.A., Şimşek, E., Korcum, A.F., & Fişkin, K. (2016). Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells. Molecular Medicine Reports, 14, 2343-2351. https://doi.org/10.3892/mmr.2016.5463
MLA
Aydemir, E. A., Şimşek, E., Korcum, A. F., Fişkin, K."Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells". Molecular Medicine Reports 14.3 (2016): 2343-2351.
Chicago
Aydemir, E. A., Şimşek, E., Korcum, A. F., Fişkin, K."Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells". Molecular Medicine Reports 14, no. 3 (2016): 2343-2351. https://doi.org/10.3892/mmr.2016.5463
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Spandidos Publications style
Aydemir EA, Şimşek E, Korcum AF and Fişkin K: Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells. Mol Med Rep 14: 2343-2351, 2016.
APA
Aydemir, E.A., Şimşek, E., Korcum, A.F., & Fişkin, K. (2016). Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells. Molecular Medicine Reports, 14, 2343-2351. https://doi.org/10.3892/mmr.2016.5463
MLA
Aydemir, E. A., Şimşek, E., Korcum, A. F., Fişkin, K."Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells". Molecular Medicine Reports 14.3 (2016): 2343-2351.
Chicago
Aydemir, E. A., Şimşek, E., Korcum, A. F., Fişkin, K."Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells". Molecular Medicine Reports 14, no. 3 (2016): 2343-2351. https://doi.org/10.3892/mmr.2016.5463
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