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Protection of rat liver against hepatic ischemia-reperfusion injury by a novel selenocysteine-containing 7-mer peptide

  • Authors:
    • Qianqian Jiang
    • Yu Pan
    • Yupeng Cheng
    • Huiling Li
    • Hui Li
  • View Affiliations / Copyright

    Affiliations: Pharmaceutical College, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China, School of Environment and Chemical Engineering, Heilongjiang University of Science and Technology, Harbin, Heilongjiang 150022, P.R. China, Department of Biochemistry and Molecular Biology, Basic Medical Science College, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2007-2015
    |
    Published online on: July 1, 2016
       https://doi.org/10.3892/mmr.2016.5464
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Abstract

Hepatic ischemia-reperfusion (I-R) injury causes acute organ damage or dysfunction, and remains a problem for liver transplantation. In the I-R phase, the generation of reactive oxygen species aggravates the injury. In the current study, a novel selenocysteine-containing 7‑mer peptide (H-Arg-Sec-Gly-Arg-Asn-Ala-Gln-OH) was constructed to imitate the active site of an antioxidant enzyme, glutathione peroxidase (GPX). The 7‑mer peptide which has a lower molecular weight, and improved water‑solubility, higher stability and improved cell membrane permeability compared with other GPX mimics. Its GPX activity reached 13 U/µmol, which was 13 times that of ebselen (a representative GPX mimic). The effect of this GPX mimic on I‑R injury of the liver was assessed in rats. The 7‑mer peptide significantly inhibited the increase in serum hepatic amino‑transferases, tissue malondialdehyde, nitric oxide contents, myeloperoxidase activity and decrease of GPX activity compared with I‑R tissue. Following treatment with the 7‑mer peptide, the expression of B‑cell CLL/lymphoma‑2 (Bcl‑2) was significantly upregulated at the mRNA and protein level compared with the I‑R group, as determined by reverse transcription‑polymerase chain reaction and immunohistochemistry, respectively. By contrast, Bcl‑2 associated X protein (Bax) was downregulated by the 7‑mer peptide compared the I‑R group. Histological and ultrastructural changes of the rat liver tissue were also compared among the experimental groups. The results of the current study suggest that the 7‑mer peptide protected the liver against hepatic I‑R injury via suppression of oxygen‑derived free radicals and regulation of Bcl‑2 and Bax expression, which are involved in the apoptosis of liver cells. The findings of the present study will further the investigation of the 7-mer peptide as an effective therapeutic agent in hepatic I-R injury.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang Q, Pan Y, Cheng Y, Li H and Li H: Protection of rat liver against hepatic ischemia-reperfusion injury by a novel selenocysteine-containing 7-mer peptide. Mol Med Rep 14: 2007-2015, 2016.
APA
Jiang, Q., Pan, Y., Cheng, Y., Li, H., & Li, H. (2016). Protection of rat liver against hepatic ischemia-reperfusion injury by a novel selenocysteine-containing 7-mer peptide. Molecular Medicine Reports, 14, 2007-2015. https://doi.org/10.3892/mmr.2016.5464
MLA
Jiang, Q., Pan, Y., Cheng, Y., Li, H., Li, H."Protection of rat liver against hepatic ischemia-reperfusion injury by a novel selenocysteine-containing 7-mer peptide". Molecular Medicine Reports 14.3 (2016): 2007-2015.
Chicago
Jiang, Q., Pan, Y., Cheng, Y., Li, H., Li, H."Protection of rat liver against hepatic ischemia-reperfusion injury by a novel selenocysteine-containing 7-mer peptide". Molecular Medicine Reports 14, no. 3 (2016): 2007-2015. https://doi.org/10.3892/mmr.2016.5464
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Q, Pan Y, Cheng Y, Li H and Li H: Protection of rat liver against hepatic ischemia-reperfusion injury by a novel selenocysteine-containing 7-mer peptide. Mol Med Rep 14: 2007-2015, 2016.
APA
Jiang, Q., Pan, Y., Cheng, Y., Li, H., & Li, H. (2016). Protection of rat liver against hepatic ischemia-reperfusion injury by a novel selenocysteine-containing 7-mer peptide. Molecular Medicine Reports, 14, 2007-2015. https://doi.org/10.3892/mmr.2016.5464
MLA
Jiang, Q., Pan, Y., Cheng, Y., Li, H., Li, H."Protection of rat liver against hepatic ischemia-reperfusion injury by a novel selenocysteine-containing 7-mer peptide". Molecular Medicine Reports 14.3 (2016): 2007-2015.
Chicago
Jiang, Q., Pan, Y., Cheng, Y., Li, H., Li, H."Protection of rat liver against hepatic ischemia-reperfusion injury by a novel selenocysteine-containing 7-mer peptide". Molecular Medicine Reports 14, no. 3 (2016): 2007-2015. https://doi.org/10.3892/mmr.2016.5464
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