Aberrant expression of anaplastic lymphoma kinase in lung adenocarcinoma: Analysis of circulating free tumor RNA using one-step reverse transcription-polymerase chain reaction

  • Authors:
    • Rossella Bruno
    • Mirella Giordano
    • Riccardo Giannini
    • Greta Alì
    • Gianfranco Puppo
    • Alessandro Ribechini
    • Antonio Chella
    • Gabriella Fontanini
  • View Affiliations

  • Published online on: July 7, 2016     https://doi.org/10.3892/mmr.2016.5479
  • Pages: 2238-2242
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Lung adenocarcinoma patients harboring anaplastic lymphoma kinase (ALK) gene rearrangements respond well to approved ALK inhibitors. However, to date, limited evidence is available regarding whether using circulating free tumor mRNA to identify aberrant ALK expression is possible, and its feasibility remains to be clearly addressed. The present study evaluated ALK expression by a one-step reverse transcription‑polymerase chain reaction (PCR) assay on the circulating free tumor mRNA from 12 lung adenocarcinoma patients. Additionally, the present study tested for ALK rearrangements by fluorescence in situ hybridization (FISH) and immunohistochemistry. A molecular genetic characterization was performed on tumor tissues and plasma samples. Aberrant ALK expression was detected in 2/12 patients using mRNA purified from plasma specimens and the results agreed with the FISH and immunohistochemistry findings of solid biopsy samples. The detection of aberrant ALK expression on circulating free tumor RNA may be feasible using a one‑step real‑time PCR assay and may be particularly helpful when a solid biopsy sample is not available.
View Figures
View References

Related Articles

Journal Cover

September-2016
Volume 14 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Bruno R, Giordano M, Giannini R, Alì G, Puppo G, Ribechini A, Chella A and Fontanini G: Aberrant expression of anaplastic lymphoma kinase in lung adenocarcinoma: Analysis of circulating free tumor RNA using one-step reverse transcription-polymerase chain reaction. Mol Med Rep 14: 2238-2242, 2016.
APA
Bruno, R., Giordano, M., Giannini, R., Alì, G., Puppo, G., Ribechini, A. ... Fontanini, G. (2016). Aberrant expression of anaplastic lymphoma kinase in lung adenocarcinoma: Analysis of circulating free tumor RNA using one-step reverse transcription-polymerase chain reaction. Molecular Medicine Reports, 14, 2238-2242. https://doi.org/10.3892/mmr.2016.5479
MLA
Bruno, R., Giordano, M., Giannini, R., Alì, G., Puppo, G., Ribechini, A., Chella, A., Fontanini, G."Aberrant expression of anaplastic lymphoma kinase in lung adenocarcinoma: Analysis of circulating free tumor RNA using one-step reverse transcription-polymerase chain reaction". Molecular Medicine Reports 14.3 (2016): 2238-2242.
Chicago
Bruno, R., Giordano, M., Giannini, R., Alì, G., Puppo, G., Ribechini, A., Chella, A., Fontanini, G."Aberrant expression of anaplastic lymphoma kinase in lung adenocarcinoma: Analysis of circulating free tumor RNA using one-step reverse transcription-polymerase chain reaction". Molecular Medicine Reports 14, no. 3 (2016): 2238-2242. https://doi.org/10.3892/mmr.2016.5479