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Article

Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction

  • Authors:
    • Li An
    • Qiaoling Lang
    • Wenbin Shen
    • Qingshui Shi
    • Fang Feng
  • View Affiliations / Copyright

    Affiliations: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China, Center for Instrumental Analysis, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China, Jiangsu Institute for Food and Drug Control, Nanjing, Jiangsu 210008, P.R. China
  • Pages: 2093-2100
    |
    Published online on: July 11, 2016
       https://doi.org/10.3892/mmr.2016.5494
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Abstract

Alcoholic liver disease (ALD) is a leading cause of liver‑associated morbidity and mortality. Zhi‑Zi‑Da‑Huang decoction (ZZDHD), a traditional Chinese medicine formula, has been frequently used to treat or alleviate the symptoms of the various stages of ALD. To identify metabolic changes and the ZZDHD mechanism of action on ALD, potential urine biomarkers involved in the effects of ZZDHD were identified. Additionally, dynamic metabolomic profiles were systematically analyzed using nuclear magnetic resonance (NMR) spectroscopy in conjunction with statistical analysis. Alcohol administration to experimental rats disrupted multiple metabolic pathways, including methionine, gut bacterial, energy and amino acid metabolism. However, ZZDHD relieved certain effects of alcohol on the metabolism and regulated changes in potential characteristic biomarkers, including dimethylglycine, hippurate, lactate and creatine. The present study investigated time‑dependent metabolomic changes in the development of alcohol‑induced liver injury, including the effect of ZZDHD intervention. These findings elucidated important information regarding the metabolic responses to the protective effects of ZZDHD. 1H NMR‑based metabolomics method a reliable and useful tool for determining the metabolic progression of alcohol‑induced liver injury and elucidating the underlying mechanisms of the effect of traditional Chinese medicine formulas. This study also demonstrated that NMR‑based metabolomics approach is a powerful tool for understanding the molecular basis of pathogenesis and drug intervention processes.
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Copy and paste a formatted citation
Spandidos Publications style
An L, Lang Q, Shen W, Shi Q and Feng F: Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction. Mol Med Rep 14: 2093-2100, 2016.
APA
An, L., Lang, Q., Shen, W., Shi, Q., & Feng, F. (2016). Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction. Molecular Medicine Reports, 14, 2093-2100. https://doi.org/10.3892/mmr.2016.5494
MLA
An, L., Lang, Q., Shen, W., Shi, Q., Feng, F."Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction". Molecular Medicine Reports 14.3 (2016): 2093-2100.
Chicago
An, L., Lang, Q., Shen, W., Shi, Q., Feng, F."Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction". Molecular Medicine Reports 14, no. 3 (2016): 2093-2100. https://doi.org/10.3892/mmr.2016.5494
Copy and paste a formatted citation
x
Spandidos Publications style
An L, Lang Q, Shen W, Shi Q and Feng F: Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction. Mol Med Rep 14: 2093-2100, 2016.
APA
An, L., Lang, Q., Shen, W., Shi, Q., & Feng, F. (2016). Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction. Molecular Medicine Reports, 14, 2093-2100. https://doi.org/10.3892/mmr.2016.5494
MLA
An, L., Lang, Q., Shen, W., Shi, Q., Feng, F."Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction". Molecular Medicine Reports 14.3 (2016): 2093-2100.
Chicago
An, L., Lang, Q., Shen, W., Shi, Q., Feng, F."Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction". Molecular Medicine Reports 14, no. 3 (2016): 2093-2100. https://doi.org/10.3892/mmr.2016.5494
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