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Article

B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages

  • Authors:
    • Lei Liu
    • Dalin Li
    • Shuang Chen
    • Ran Zhao
    • Da Pang
    • Dianjun Li
    • Zhenkun Fu
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, Heilongjiang Provincial Key Laboratory for Infection and Immunity, Heilongjiang Academy of Medical Sciences, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Breast Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China
  • Pages: 2135-2142
    |
    Published online on: July 13, 2016
       https://doi.org/10.3892/mmr.2016.5510
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Abstract

B7-H4 is a co‑inhibitory molecule of the B7 family, which is expressed on antigen‑presenting cells (APCs) and is able to limit the T‑cell immune response. Macrophages act as professional APCs and are important for immunoregulation of the tumor microenvironment in breast cancer. In order to identify the association between the presence of B7‑H4 on macrophages and infiltrating ductal carcinoma (IDC), the present study investigated the expression of B7‑H4 on macrophages with different polarizations. The expression levels of B7‑H4 in IDC tissues were determined using immunohistochemistry, and the expression of B7‑H4 on macrophages in the breast IDC microenvironment were determined using western blot analysis and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The expression levels of interleukin (IL)‑6 and IL‑10 were detected in IDC tissues and the supernatants of polarized macrophages using an enzyme‑linked immunosorbent assay and RT‑qPCR. The present study demonstrated that B7‑H4 was overexpressed in IDC tissues and macrophages. In vitro, M1 and M2 macrophages exhibited different expression levels of B7‑H4. IL‑6 and ‑10 exhibited higher expression in the IDC tissues compared with in distal pericarcinomatous tissues. In conclusion, B7‑H4 exhibited overexpression in IDC tissues and cultured macrophage cells. Furthermore, M2 macrophages exhibited higher expression levels of B7‑H4 compared with the M1 subtype. In addition, IL‑6 and ‑10 may be associated with B7‑H4 expression on macrophages of different polarizations in the IDC microenvironment.
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Copy and paste a formatted citation
Spandidos Publications style
Liu L, Li D, Chen S, Zhao R, Pang D, Li D and Fu Z: B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages. Mol Med Rep 14: 2135-2142, 2016.
APA
Liu, L., Li, D., Chen, S., Zhao, R., Pang, D., Li, D., & Fu, Z. (2016). B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages. Molecular Medicine Reports, 14, 2135-2142. https://doi.org/10.3892/mmr.2016.5510
MLA
Liu, L., Li, D., Chen, S., Zhao, R., Pang, D., Li, D., Fu, Z."B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages". Molecular Medicine Reports 14.3 (2016): 2135-2142.
Chicago
Liu, L., Li, D., Chen, S., Zhao, R., Pang, D., Li, D., Fu, Z."B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages". Molecular Medicine Reports 14, no. 3 (2016): 2135-2142. https://doi.org/10.3892/mmr.2016.5510
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Li D, Chen S, Zhao R, Pang D, Li D and Fu Z: B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages. Mol Med Rep 14: 2135-2142, 2016.
APA
Liu, L., Li, D., Chen, S., Zhao, R., Pang, D., Li, D., & Fu, Z. (2016). B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages. Molecular Medicine Reports, 14, 2135-2142. https://doi.org/10.3892/mmr.2016.5510
MLA
Liu, L., Li, D., Chen, S., Zhao, R., Pang, D., Li, D., Fu, Z."B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages". Molecular Medicine Reports 14.3 (2016): 2135-2142.
Chicago
Liu, L., Li, D., Chen, S., Zhao, R., Pang, D., Li, D., Fu, Z."B7-H4 expression in human infiltrating ductal carcinoma‑associated macrophages". Molecular Medicine Reports 14, no. 3 (2016): 2135-2142. https://doi.org/10.3892/mmr.2016.5510
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