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Article

Functional study of the upregulation of miRNA-27a and miRNA-27b in 3T3-L1 cells in response to berberine

  • Authors:
    • Yue‑Yue Wu
    • Xin‑Mei Huang
    • Jun Liu
    • Ying Cha
    • Zao‑Ping Chen
    • Fang Wang
    • Jiong Xu
    • Li Sheng
    • He‑Yuang Ding
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The Fifth People's Hospital of Shanghai Affiliated to Fudan University, Shanghai 200240, P.R. China
  • Pages: 2725-2731
    |
    Published online on: July 25, 2016
       https://doi.org/10.3892/mmr.2016.5545
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Abstract

Berberine is the major active component of Rhizoma Coptidis derived from a traditional Chinese herbal medicine and is known to regulate micro (mi)RNA levels, although the mechanism for this action remains unknown. The present study confirmed that treatment of 3T3‑L1 cells with berberine inhibited cell viability and differentiation in a dose‑ and time‑dependent manner, and significantly increased the mRNA expression levels of miRNA‑27a and miRNA‑27b. In addition, in 3T3‑L1 cells treated with berberine, overexpression of miRNA‑27a and miRNA‑27b improved the berberine-mediated inhibition of cell differentiation and reduction of triglyceride contents. By contrast, miRNA‑27a and miRNA‑27b inhibitors attenuated the berberine‑mediated inhibition of cell differentiation and reduction of triglyceride contents. Additionally, peroxisome proliferator‑activated receptors (PPAR)‑γ was confirmed to be a target of miRNA‑27a in the 3T3‑L1 cells. A dual‑luciferase reporter assay indicated that the expression of PPAR‑γ was negatively regulated by miRNA-27a. These findings may provide novel mechanistic insight into the antiobesity effects of certain compounds in traditional Chinese herbal medicine.
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Copy and paste a formatted citation
Spandidos Publications style
Wu YY, Huang XM, Liu J, Cha Y, Chen ZP, Wang F, Xu J, Sheng L and Ding HY: Functional study of the upregulation of miRNA-27a and miRNA-27b in 3T3-L1 cells in response to berberine. Mol Med Rep 14: 2725-2731, 2016.
APA
Wu, Y., Huang, X., Liu, J., Cha, Y., Chen, Z., Wang, F. ... Ding, H. (2016). Functional study of the upregulation of miRNA-27a and miRNA-27b in 3T3-L1 cells in response to berberine. Molecular Medicine Reports, 14, 2725-2731. https://doi.org/10.3892/mmr.2016.5545
MLA
Wu, Y., Huang, X., Liu, J., Cha, Y., Chen, Z., Wang, F., Xu, J., Sheng, L., Ding, H."Functional study of the upregulation of miRNA-27a and miRNA-27b in 3T3-L1 cells in response to berberine". Molecular Medicine Reports 14.3 (2016): 2725-2731.
Chicago
Wu, Y., Huang, X., Liu, J., Cha, Y., Chen, Z., Wang, F., Xu, J., Sheng, L., Ding, H."Functional study of the upregulation of miRNA-27a and miRNA-27b in 3T3-L1 cells in response to berberine". Molecular Medicine Reports 14, no. 3 (2016): 2725-2731. https://doi.org/10.3892/mmr.2016.5545
Copy and paste a formatted citation
x
Spandidos Publications style
Wu YY, Huang XM, Liu J, Cha Y, Chen ZP, Wang F, Xu J, Sheng L and Ding HY: Functional study of the upregulation of miRNA-27a and miRNA-27b in 3T3-L1 cells in response to berberine. Mol Med Rep 14: 2725-2731, 2016.
APA
Wu, Y., Huang, X., Liu, J., Cha, Y., Chen, Z., Wang, F. ... Ding, H. (2016). Functional study of the upregulation of miRNA-27a and miRNA-27b in 3T3-L1 cells in response to berberine. Molecular Medicine Reports, 14, 2725-2731. https://doi.org/10.3892/mmr.2016.5545
MLA
Wu, Y., Huang, X., Liu, J., Cha, Y., Chen, Z., Wang, F., Xu, J., Sheng, L., Ding, H."Functional study of the upregulation of miRNA-27a and miRNA-27b in 3T3-L1 cells in response to berberine". Molecular Medicine Reports 14.3 (2016): 2725-2731.
Chicago
Wu, Y., Huang, X., Liu, J., Cha, Y., Chen, Z., Wang, F., Xu, J., Sheng, L., Ding, H."Functional study of the upregulation of miRNA-27a and miRNA-27b in 3T3-L1 cells in response to berberine". Molecular Medicine Reports 14, no. 3 (2016): 2725-2731. https://doi.org/10.3892/mmr.2016.5545
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