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Article

Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study

  • Authors:
    • Essam Hassan Mohamed
    • Ahmed Abdel‑Aziz Baiomy
    • Zein Shaban Ibrahim
    • Mohamed Mohamed Soliman
  • View Affiliations / Copyright

    Affiliations: Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt, Department of Zoology, Faculty of Science, Cairo University, Giza 12613, Egypt, Department of Physiology, College of Medicine, Taif University, Taif 21944, Saudi Arabia, Department of Medical Laboratories, Faculty of Applied Medical Sciences, Taif University, Turabah 21974, Saudi Arabia
  • Pages: 2755-2763
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    Published online on: July 27, 2016
       https://doi.org/10.3892/mmr.2016.5551
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Abstract

Levamisole (LEVA) and garlic are prevalent immunomodulators in humans and animals. Therefore, the present study aimed to examine the immunomodulatory effects of LEVA and garlic oil (GO) alone or in combination on the immune response of Wistar rats. A total of 24 male Wistar rats were allocated into four equal groups: Control group, which was given ad libitum access to food and water; and groups 2‑4, which were orally administered LEVA [2.5 mg/kg body weight (BW) every 2 days], GO, (5 ml/kg BW daily), or LEVA plus GO, respectively for 4 consecutive weeks. Serum immunoglobulin (Ig)G and IgM levels were measured using a radial immunodiffusion assay. Serum cytokine levels, including interferon (IFN)-γ, interleukin (IL)-5 and tumor necrosis factor (TNF)-α, were measured using enzyme‑linked immunosorbent assay kits. Total blood counts were measured automatically using a cell counter. Serum lysozyme enzymatic activity was determined by measuring the diameters of the zones of clearance relative to lysozyme. Immunohistochemical detection of CD4 and CD8 was carried out using the streptavidin-biotin-peroxidase method. Furthermore, the mRNA expression levels of IL‑4, IL‑5 and IL‑12 were measured in the leukocytes and thymus gland by semi-quantitative polymerase chain reaction. The results revealed that LEVA increased serum levels of IFN‑γ, IL‑5 and TNF‑α cytokines, whereas co‑administration of LEVA and GO decreased the stimulatory action of LEVA alone. LEVA and GO alone increased the serum levels of IgG, IgM and total blood cell counts, and co‑administration of GO and LEVA inhibited the effects of LEVA. At the cellular level, in the spleen, LEVA increased immunoreactivity of CD4 and CD8, whereas co‑administration of GO with LEVA decreased this strong expression. At the molecular level, in leukocytes, LEVA upregulated the mRNA expression levels of IL‑2, IL‑4 and IL‑5, whereas GO alone downregulated mRNA expression. Co‑administration of GO with LEVA inhibited the LEVA‑induced upregulation of IL‑2, IL‑4 and IL‑5 mRNA expression. In the thymus, both LEVA and GO upregulated the mRNA expression levels of IL‑4 and IL‑5, whereas LEVA alone did not affect IL‑12 mRNA expression. Co‑administration of GO with LEVA inhibited LEVA‑induced upregulation of IL‑4 and GO‑induced upregulation of IL‑12 expression, and had an additive upregulatory effect on IL‑5 expression. In conclusion, LEVA stimulated T‑helper (Th)1 cytokines, whereas GO stimulated a Th2 response, and co‑administration of GO with LEVA inhibited the stimulatory effects of LEVA and balanced the Th1/Th2 response.
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1 

Page DB, Postow MA, Callahan MK, Allison JP and Wolchok JD: Immune modulation in cancer with antibodies. Annual Review Med. 65:185–202. 2014. View Article : Google Scholar

2 

Agrawal SS, Khadase SC and Talele GS: Studies on immunomodulatory activity of Capparis zeylanica leaf extracts. International Journal of Pharmaceutical Sciences and Nanotechnology. 3:887–892. 2010.

3 

Kandil OM, Abdellah TH and Elkadi A: Garlic and the immune system in humans: Its effects on natural killer cells. Federal Procedures. 46:4411987.

4 

Butt MS, Sultan MT, Butt MS and Iqbal J: Garlic nature's protection against physiological threats. Crit Rev Sci Nutr. 49:538–551. 2009. View Article : Google Scholar

5 

Kyo E, Uda N, Suzuki A, Kakimoto M, Ushijima M, Kasuga S and Itakura Y: Immunomodulatory effects of aged garlic extract. J Nutr. 131:1075S–1079S. 2001.PubMed/NCBI

6 

Ghazanfari T, Hassan ZM, Ebtekar M, Ahmadiani A, Naderi G and Azar A: Garlic induces a shift in cytokine pattern in leishmania major-infected BALB/c mice. Scand J Immunol. 52:491–495. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Chandrashekar PM and Venkatesh YP: Identification of the protein components displaying immunomodulatory activity in aged garlic extract. J Ethnopharmacolol. 124:384–390. 2009. View Article : Google Scholar

8 

Ebrahimi M, Mohammad Hassan Z, Mostafaie A, Zare Mehrjardi N and Ghazanfari T: Purified protein fraction of garlic extract modulates cellular immune response against breast transplanted tumors in BALB/c mice model. Cell J. 15:65–75. 2013.PubMed/NCBI

9 

Chadwick RG, Jain S, Cohen BJ, Scott GM, Thomas HC and Sherlock S: Levamisole therapy for HbsAg-positive chronic liver disease. Scand J Gastroenterol. 15:973–978. 1980. View Article : Google Scholar : PubMed/NCBI

10 

Fox MT, Jacobs DE, Campling RC, Pocknee BR, Clampitt R and Hart IC: Effect of thiabendazole treatment on feed intake, digetability and selected blood values in lactating dairy cows. Vet Rec. 116:257–260. 1985. View Article : Google Scholar : PubMed/NCBI

11 

Mitchell MS: Immunotherapy as part of combinations for the treatment of cancer. Int Immunopharmacol. 3:1051–1059. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Szeto C, Gillespie KM and Mathieson PW: Levamisole induces interleukin-18 and shifts type 1/type 2 cytokine balance. Immunology. 100:217–224. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Olusi SO, Jessop WJE and Shoroye A: Effects of levamisole on the immune response of experimentally malnourished rats. Pediatric Res. 13:1237–1239. 1979. View Article : Google Scholar

14 

Kocabas CN, Sekerel BE, Firat PA, Okur H and Adahoglu G: Levamisole: Might it be used in treatment and prevention of atopic diseases? J Asthma. 41:547–551. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Chen LY, Lin YL and Chiang BL: Levamisole enhances immune response by affecting the activation and maturation of human monocyte-derived dendritic cells. Clin Exp Immunol. 151:174–181. 2008. View Article : Google Scholar

16 

Mulero V, Esteban MA, Munxoz J and Meseguer J: Dietary intake of levamisole enhances the immune response and disease resistance of the marine teleost gilthead seabream (Sparus aurata L). Fish and Shellfish Immunology. 8:49–62. 1998. View Article : Google Scholar

17 

Ali HS, El-Sanosi YA and Shaimaa A: Biochemical, immunomodulatory and antioxidant proportes of levamisole at different storage conditions and administration routes. Pak J Biol Sci. 15:986–991. 2012. View Article : Google Scholar

18 

Hassan HA, Hafez HS and Zeghebar FE: Garlic oil as a modulating agent for oxidative stress and neurotoxicity induced by sodium nitrite in male albino rats. Food Chem Toxicol. 48:1980–1985. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Osserman EF and Lawlor DP: Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med. 124:921–952. 1966. View Article : Google Scholar : PubMed/NCBI

20 

Bancroft JD and Gamble M: Theory and practice of histological techniques. 6th ed. Churchill Livingstone Elsevier; Philadelphia: pp. 126–127. 2008

21 

Cho Y, Miyamoto M, Kato K, Fukunaga A, Shichinohe T, Kawarada Y, Hida Y, Oshikiri T, Kurokawa T, Suzuoki M, et al: CD4+ and CD8+ T cells cooperate to improve prognosis of patients with esophageal squamous cell carcinoma. Cancer Res. 63:1555–1559. 2003.PubMed/NCBI

22 

Soliman MM, Nassan MA and Ismail TA: Immunohistochemical and molecular study on the protective effect of curcumin against hepatic toxicity induced by paracetamol in Wistar rats. BMC Complement Altern Med. 14:4572014. View Article : Google Scholar : PubMed/NCBI

23 

De Araújo-Souza PS, Hanschke SC and Viola JPJ: Epigenetic control of interferon-gamma expression in CD8 T cells. J Immunol Res. 2015:8495732015. View Article : Google Scholar : PubMed/NCBI

24 

Schroder K, Hertzog PJ, Ravasi T and Hume DA: Interferon-gamma: An overview of signals, mechanisms and functions. J Leukoc Biol. 75:163–189. 2004. View Article : Google Scholar

25 

Heurtier AH and Boitard C: T-cell regulation in murine and human autoimmune diabetes: The role of TH1 and TH2 cells. Diabetes Metab. 23:377–385. 1997.

26 

Kay AB: TH2-type cytokines in asthma. Ann N Y Acad Sci. 796:1–8. 1996. View Article : Google Scholar : PubMed/NCBI

27 

Sung J, Harfouche Y, De La Cruz M, Zamora MP, Liu Y, Rego JA and Buckley NE: Garlic (Allium sativum) stimulates lipopoly-saccharide-induced tumor necrosis factor-alpha production from J774A.1 murine macrophages. Phytother Res. 29:288–294. 2015. View Article : Google Scholar

28 

Nair P: What is an 'eosinophilic phenotype' of asthma? J Allergy Clin Immunol. 132:81–83. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Gleich GJ: Mechanisms of eosinophil-associated inflammation. J Allergy Clin Immuol. 105:651–663. 2000. View Article : Google Scholar

30 

Garcia G, Taillé C, Laveneziana P, Bourdin A, Chanez P and Humbert M: Antiinterleukin-5 therapy in severe asthma. Eur Respir Rev. 22:251–257. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Yoo JM, Sok DE and Kim MR: Anti-allergic action of aged black garlic extract in RBL-2H3 cells and passive cutaneous anaphylaxis reaction in mice. J Med Food. 17:92–102. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Pekmezci D and Cakiroglu D: Investigation of immunmodulatory effects of levamisole and vitamin E on immunity and some blood parameters in newborn Jersey calves. Vet Res Commun. 33:711–721. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Budoff MJ, Ahmadi N, Gul KM, Liu ST, Flores FR, Tiano J, Takasu J, Miller E and Tsimikas S: Aged garlic extract supplemented with B vitamins, folic acid and L-arginine retards the progression of subclinical atherosclerosis: A randomized clinical trial. Prev Med. 49:101–107. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Chekwube AI, Onyema EI, Ikenna UE and Ezeokonkwo RC: Effect of diminazene aceturate, levamisole and vitamin C combination therapy in rats experimentally infected with Trypanosoma brucei brucei. Asian Pac Trop Med. 7:438–445. 2014. View Article : Google Scholar

35 

Dong Diegane N and Fall Jean: The effect of garlic (Allium sativum) on growth and immune responses of hybrid tilapia (Oreochromis niloticus × Oreochromis aureus. Journal of Clinical Immnunology and Immunopathology Research. 3:1–9. 2011.

36 

Jollès P and Jollès J: What's new in lysozyme research? Always a model system, today as yeas day. Mol Cell Biochem. 63:165–189

Jorgensen J and Robertsen B: 1995.Yeast_-glucan stimulates respiratory burst activity of Atlantic salmon (Salmo salar L) macrophages. Dev Comp Immunol. 19:43–157. 1984. View Article : Google Scholar

37 

Jianu CS, Lange OJ, Viset T, Qvigstad G, Martinsen TC, Fougner R, Kleveland PM, Fossmark R, Hauso Ø and Waldum HL: Gastric neuroendocrine carcinoma after long-term use of proton pump inhibitor. Scan J Gastroenterol. 47:64–67. 2012. View Article : Google Scholar

38 

Mayeux PR, Agrawal KC, Tou JS, King BT, Lippton HL, Hyman AL, Kadowitz PJ and McNamara DB: The pharmacological effects of allicin, a constituent of garlic oil. Agents Actions. 25:182–190. 1988. View Article : Google Scholar : PubMed/NCBI

39 

Sayad B, Alavian SM, Najafi F, Soltani B, Shirvani M, Janbakhsh A, Mansouri F, Afsharian M, Vaziri S, Alikhani A and Bashiri H: Effects of Oral Levamisole as an adjuvant to hepatitis B vaccine in HIV/AIDS patients: A randomized controlled trial. Hepat Mon. 12:e62342012. View Article : Google Scholar

40 

Prakash MS, Rao VM and Reddy V: Effect of levamisole on the immune status of malnourished children. J Trop Pediatr. 44:165–166. 1998. View Article : Google Scholar : PubMed/NCBI

41 

Cantor HEA and Boyse: Regulation of cellular and humoral immune responses by T-cell subclasses. Cold Spring Harb Symp Quant Biol. 41:23–32. 1977. View Article : Google Scholar : PubMed/NCBI

42 

You S, Nakanishi E, Kuwata H, Chen J, Nakasone Y, He X, He J, Liu X, Zhang S, Zhang B and Hou DX: Inhibitory effects and molecular mechanisms of garlic organosulfur compounds on the production of inflammatory mediators. Mol Nutr Food Res. 57:2049–2060. 2013. View Article : Google Scholar : PubMed/NCBI

43 

Nizzoli G1, Krietsch J, Weick A, Steinfelder S, Facciotti F, Gruarin P, Bianco A, Steckel B, Moro M, Crosti M, et al: Human CD1c+ dendritic cells secrete high levels of IL-12 and potently prime cytotoxic T-cell responses. Blood. 122:932–942. 2013. View Article : Google Scholar : PubMed/NCBI

44 

Gharavi M, Nobakht M, Khademvatan S, Fani F, Bakhshayesh M and Roozbehani M: The Effect of aqueous garlic extract on interleukin-12 and 10 levels in leishmania major (MRHO/IR/75/ER) infected macrophages. Iran J Public Health. 40:105–111. 2011.

45 

Burton OT, Darling AR, Zhou JS, Noval-Rivas M, Jones TG, Gurish MF, Chatila TA and Oettgen HC: Direct effects of IL-4 on mast cells drive their intestinal expansion and increase susceptibility to anaphylaxis in a murine model of food allergy. Mucosal Immunol. 6:740–750. 2013. View Article : Google Scholar :

46 

Liao W, Lin JX and Leonard WJ: Interleukin-2 at the crossroads of effector responses, tolerance and immunotherapy. Immunity. 38:13–25. 2013. View Article : Google Scholar : PubMed/NCBI

47 

Mantawy MM, Ali HF and Rizk MZ: Therapeutic effects of Allium sativum and Allium cepa in Schistosoma mansoni experimental infection. Revista Inst Med Trop Sao Paulo. 53:155–163. 2011. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Mohamed EH, Baiomy AA, Ibrahim ZS and Soliman MM: Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study. Mol Med Rep 14: 2755-2763, 2016.
APA
Mohamed, E.H., Baiomy, A.A., Ibrahim, Z.S., & Soliman, M.M. (2016). Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study. Molecular Medicine Reports, 14, 2755-2763. https://doi.org/10.3892/mmr.2016.5551
MLA
Mohamed, E. H., Baiomy, A. A., Ibrahim, Z. S., Soliman, M. M."Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study". Molecular Medicine Reports 14.3 (2016): 2755-2763.
Chicago
Mohamed, E. H., Baiomy, A. A., Ibrahim, Z. S., Soliman, M. M."Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study". Molecular Medicine Reports 14, no. 3 (2016): 2755-2763. https://doi.org/10.3892/mmr.2016.5551
Copy and paste a formatted citation
x
Spandidos Publications style
Mohamed EH, Baiomy AA, Ibrahim ZS and Soliman MM: Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study. Mol Med Rep 14: 2755-2763, 2016.
APA
Mohamed, E.H., Baiomy, A.A., Ibrahim, Z.S., & Soliman, M.M. (2016). Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study. Molecular Medicine Reports, 14, 2755-2763. https://doi.org/10.3892/mmr.2016.5551
MLA
Mohamed, E. H., Baiomy, A. A., Ibrahim, Z. S., Soliman, M. M."Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study". Molecular Medicine Reports 14.3 (2016): 2755-2763.
Chicago
Mohamed, E. H., Baiomy, A. A., Ibrahim, Z. S., Soliman, M. M."Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study". Molecular Medicine Reports 14, no. 3 (2016): 2755-2763. https://doi.org/10.3892/mmr.2016.5551
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