miR-22-5p revealed as a potential biomarker involved in the acute phase of myocardial infarction via profiling of circulating microRNAs

Maciejak,Agata; Kiliszek,Marek; Opolski,Grzegorz; Segiet,Agnieszka; Matlak,Krzysztof; Dobrzycki,Slawomir; Tulacz,Dorota; Sygitowicz,Grazyna; Burzynska,Beata; Gora,Monika

doi:10.3892/mmr.2016.5566

September-2016 Volume 14 Issue 3

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September-2016 Volume 14 Issue 3

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Article

miR-22-5p revealed as a potential biomarker involved in the acute phase of myocardial infarction via profiling of circulating microRNAs

Authors:
- Agata Maciejak
- Marek Kiliszek
- Grzegorz Opolski
- Agnieszka Segiet
- Krzysztof Matlak
- Slawomir Dobrzycki
- Dorota Tulacz
- Grazyna Sygitowicz
- Beata Burzynska
- Monika Gora
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Affiliations: Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02‑106 Warsaw, Poland, Department of Cardiology and Internal Diseases, Military Institute of Medicine, 04‑141 Warsaw, Poland, 1st Chair and Department of Cardiology, Medical University of Warsaw, 02‑091 Warsaw, Poland, 1st Faculty of Medicine, Medical University of Warsaw, 02‑091 Warsaw, Poland, Department of Cardiac Surgery, Medical University of Bialystok, 15‑089 Bialystok, Poland, Department of Invasive Cardiology, Medical University of Bialystok, 15‑089 Bialystok, Poland, Department of Medical Laboratory Diagnostics, Medical University of Warsaw, 02‑091 Warsaw, Poland
Pages: 2867-2875
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Published online on: July 27, 2016

https://doi.org/10.3892/mmr.2016.5566
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Abstract

Acute myocardial infarction (AMI) is a life-threatening episode of coronary artery disease. Recently, circulating myocardial-derived microRNAs (miRNAs) have been reported as potential biomarkers of infarction. The present study aimed to identify differentially expressed miRNAs in patients with ST-segment elevation myocardial infarction that could be potentially dysregulated in response to early myocardial damage. miRNA expression profile analysis was performed using the Serum/Plasma Focus miRNA Polymerase Chain Reaction (PCR) panel of Exiqon A/S (Vedbaek, Denmark) on plasma samples of patients on the first day of AMI (admission) and on samples from the identical patients collected six months following AMI. Selected miRNAs were validated by reverse transcription‑quantitative PCR (RT‑qPCR) using independent patients with AMI and a control group of patients with a stable coronary artery disease. Thirty‑two species of plasma miRNA were differentially expressed (P<0.05) on admission compared with six months following AMI. Subsequent validation in an independent patient group confirmed that miR‑133b and miR‑22‑5p were significantly up‑regulated in the serum of patients with AMI. The receiver operating characteristic (ROC) curve analysis demonstrated a diagnostic utility for miR-22-5p, which has not previously been reported to be associated with AMI. Among the selected miRNAs, miR‑22‑5p represents a novel promising biomarker for the diagnosis of AMI.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

miR-22-5p revealed as a potential biomarker involved in the acute phase of myocardial infarction via profiling of circulating microRNAs

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