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Article

Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy

  • Authors:
    • Rui‑Xia Zhu
    • Chun‑Hui Song
    • Jin‑Shan Yang
    • Qing‑Ting Yi
    • Bao‑Jian Li
    • Si‑Hai Liu
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Central Hospital of Zaozhuang Mineral Group, Zaozhuang, Shandong 277800, P.R. China
  • Pages: 2846-2852
    |
    Published online on: August 1, 2016
       https://doi.org/10.3892/mmr.2016.5579
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Abstract

The deregulation of microRNAs (miRNAs) is often implicated in the control of sensitivity to radiotherapy. The objective of the present study was to identify the association between miR‑558 and apoptosis‑associated tyrosine kinase (AATK), and their importance in regulating the development of resistance to radiotherapy. The current study demonstrated that AATK, a radiosensitization-associated gene, is a target of miR‑558 in lung cancer cells, using in silico analysis and a luciferase reporter system. Furthermore, it was determined that transfection of 30 or 50 nM miR‑558 mimics and AATK specific siRNA markedly suppressed the mRNA and protein expression of AATK. To determine whether miR‑558 was required for lung cancer cell radioresistance, A549 cells were treated with different doses of ionizing radiation, from 0 to 10 Gy, following transfection with miR‑558 mimics or AATK specific siRNA. It was determined that the administration of miR‑558 mimics or AATK specific siRNA alone did not significantly alter the survival rate of the cells. By contrast, in the cells exposed to 4, 6 or 8 Gy, the administration of miR‑558 mimics or AATK specific siRNA significantly promoted cell survival rate and overexpression of AATK reversed this effect. In conclusion, these data demonstrate that the miR‑558/AATK cascade is important for the radiosensitization of lung cancer cells and may be a potential radiotherapy target.
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1 

Sun X, Liu W, Wu S, Han H, Lin Y and Dai X: The morbidity and mortality trend and prediction of lung cancer in residents of Nangang District of Harbin in China during the past 10 years. Zhongguo Fei Ai Za Zhi. 8:514–517. 2005.PubMed/NCBI

2 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2012. CA Cancer J Clin. 62:10–29. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Fidias P and Novello S: Strategies for prolonged therapy in patients with advanced non-small-cell lung cancer. J Clin Oncol. 28:5116–5123. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Whitehurst AW, Bodemann BO, Cardenas J, Ferguson D, Girard L, Peyton M, Minna JD, Michnoff C, Hao W, Roth MG, et al: Synthetic lethal screen identification of chemosensitizer loci in cancer cells. Nature. 446:815–819. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Blackstock AW and Govindan R: Definitive chemoradiation for the treatment of locally advanced non small-cell lung cancer. J Clin Oncol. 25:4146–4152. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Salama JK and Vokes EE: New radiotherapy and chemoradiotherapy approaches for non-small-cell lung cancer. J Clin Oncol. 31:1029–1038. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Le Pechoux C: Role of postoperative radiotherapy in resected non-small cell lung cancer: A reassessment based on new data. Oncologist. 16:672–681. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Barcellos-Hoff MH, Park C and Wright EG: Radiation and the microenvironment-tumorigenesis and therapy. Nat Rev Cancer. 5:867–875. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Madani I, De Neve W and Mareel M: Does ionizing radiation stimulate cancer invasion and metastasis? Bull Cancer. 95:292–300. 2008.PubMed/NCBI

10 

McBride WH, Chiang CS, Olson JL, Wang CC, Hong JH, Pajonk F, Dougherty GJ, Iwamoto KS, Pervan M and Liao YP: A sense of danger from radiation. Radiat Res. 162:1–19. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Rödel F, Hoffmann J, Distel L, Herrmann M, Noisternig T, Papadopoulos T, Sauer R and Rödel C: Survivin as a radioresistance factor and prognostic and therapeutic target for radiotherapy in rectal cancer. Cancer Res. 65:4881–4887. 2005. View Article : Google Scholar

12 

Lee S, Lim MJ, Kim MH, Yu CH, Yun YS, Ahn J and Song JY: An effective strategy for increasing the radiosensitivity of human lung cancer cells by blocking Nrf2-dependent antioxidant responses. Free Radic Biol Med. 53:807–816. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Provencio M, Sánchez A, Garrido P and Valcárcel F: New molecular targeted therapies integrated with radiation therapy in lung cancer. Clin Lung Cancer. 11:91–97. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Danesi R, Pasqualetti G, Giovannetti E, Crea F, Altavilla G, Del Tacca M and Rosell R: Pharmacogenomics in non-small-cell lung cancer chemotherapy. Adv Drug Deliv Rev. 61:408–417. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Hutvagner G and Zamore PD: A microRNA in a multiple-turnover RNAi enzyme complex. Science. 297:2056–2060. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Ketting RF, Fischer SE, Bernstein E, Sijen T, Hannon GJ and Plasterk RH: Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev. 15:2654–2659. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Westermann F and Schwab M: Genetic parameters of neuroblastomas. Cancer Lett. 184:127–147. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Dewey WC, Ling CC and Meyn RE: Radiation-induced apoptosis: Relevance to radiotherapy. Int J Radiat Oncol Biol Phys. 33:781–796. 1995. View Article : Google Scholar : PubMed/NCBI

19 

Ma S and Rubin BP: Apoptosis-associated tyrosine kinase 1 inhibits growth and migration and promotes apoptosis in melanoma. Lab Invest. 94:430–438. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Purnell PR, Mack PC, Tepper CG, Evans CP, Green TP, Gumerlock PH, Lara PN, Gandara DR, Kung HJ and Gautschi O: The Src inhibitor AZD0530 blocks invasion and may act as a radiosensitizer in lung cancer cells. J Thorac Oncol. 4:448–454. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Kos A, Olde Loohuis NF, Wieczorek ML, Glennon JC, Martens GJ, Kolk SM and Aschrafi A: A potential regulatory role for intronic microRNA-338-3p for its host gene encoding apoptosis-associated tyrosine kinase. PLoS One. 7:e310222012. View Article : Google Scholar : PubMed/NCBI

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

23 

Adie EA, Adams RR, Evans KL, Porteous DJ and Pickard BS: SUSPECTS: Enabling fast and effective prioritization of positional candidates. Bioinformatics. 22:773–774. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Vlodavsky I, Ilan N, Naggi A and Casu B: Heparanase: Structure, biological functions and inhibition by heparin-derived mimetics of heparan sulfate. Curr Pharm Des. 13:2057–2073. 2007. View Article : Google Scholar

25 

Zetser A, Bashenko Y, Edovitsky E, Levy-Adam F, Vlodavsky I and Ilan N: Heparanase induces vascular endothelial growth factor expression: Correlation with p38 phosphorylation levels and Src activation. Cancer Res. 66:1455–1463. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Parsons SJ and Parsons JT: Src family kinases, key regulators of signal transduction. Oncogene. 23:7906–7909. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Mazurenko NN, Kogan EA, Zborovskaya IB and Kisseljov FL: Expression of pp60c-src in human small cell and non-small cell lung carcinomas. Eur J Cancer. 28:372–377. 1992. View Article : Google Scholar : PubMed/NCBI

28 

Irby RB, Mao W, Coppola D, Kang J, Loubeau JM, Trudeau W, Karl R, Fujita DJ, Jove R and Yeatman TJ: Activating SRC mutation in a subset of advanced human colon cancers. Nat Genet. 21:187–190. 1999. View Article : Google Scholar : PubMed/NCBI

29 

Kiefer PE, Bepler G, Kubasch M and Havemann K: Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47:6236–6242. 1987.PubMed/NCBI

30 

Navarro A, Beà S, Fernández V, Prieto M, Salaverria I, Jares P, Hartmann E, Mozos A, López-Guillermo A, Villamor N, et al: MicroRNA expression, chromosomal alterations and immunoglobulin variable heavy chain hypermutations in mantle cell lymphomas. Cancer Res. 69:7071–7078. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Chen G, Zhu W, Shi D, Lv L, Zhang C, Liu P and Hu W: MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2. Oncol Rep. 23:997–1003. 2010.PubMed/NCBI

32 

Chun-Zhi Z, Lei H, An-Ling Z, Yan-Chao F, Xiao Y, Guang-Xiu W, Zhi-Fan J, Pei-Yu P, Qing-Yu Z and Chun-Sheng K: MicroRNA-221 and microRNA-222 regulate gastric carcinoma cell proliferation and radioresistance by targeting PTEN. BMC Cancer. 10:3672010. View Article : Google Scholar : PubMed/NCBI

33 

Arora H, Qureshi R, Jin S, Park AK and Park WY: miR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NFκB1. Exp Mol Med. 43:298–304. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LA Jr, Sjoblom T, Barad O, Bentwich Z, Szafranska AE, Labourier E, et al: The colorectal microRNAome. Proc Natl Acad Sci USA. 103:3687–3692. 2006. View Article : Google Scholar : PubMed/NCBI

35 

Maes OC, An J, Sarojini H, Wu H and Wang E: Changes in MicroRNA expression patterns in human fibroblasts after low-LET radiation. J Cell Biochem. 105:824–834. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Si H, Sun X, Chen Y, Cao Y, Chen S, Wang H and Hu C: Circulating microRNA-92a and microRNA-21 as novel minimally invasive biomarkers for primary breast cancer. J Cancer Res Clin Oncol. 139:223–229. 2013. View Article : Google Scholar :

37 

Shohet JM, Ghosh R, Coarfa C, Ludwig A, Benham AL, Chen Z, Patterson DM, Barbieri E, Mestdagh P, Sikorski DN, et al: A genome-wide search for promoters that respond to increased MYCN reveals both new oncogenic and tumor suppressor microRNAs associated with aggressive neuroblastoma. Cancer Res. 71:3841–3851. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhu RX, Song CH, Yang JS, Yi QT, Li BJ and Liu SH: Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy. Mol Med Rep 14: 2846-2852, 2016.
APA
Zhu, R., Song, C., Yang, J., Yi, Q., Li, B., & Liu, S. (2016). Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy. Molecular Medicine Reports, 14, 2846-2852. https://doi.org/10.3892/mmr.2016.5579
MLA
Zhu, R., Song, C., Yang, J., Yi, Q., Li, B., Liu, S."Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy". Molecular Medicine Reports 14.3 (2016): 2846-2852.
Chicago
Zhu, R., Song, C., Yang, J., Yi, Q., Li, B., Liu, S."Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy". Molecular Medicine Reports 14, no. 3 (2016): 2846-2852. https://doi.org/10.3892/mmr.2016.5579
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu RX, Song CH, Yang JS, Yi QT, Li BJ and Liu SH: Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy. Mol Med Rep 14: 2846-2852, 2016.
APA
Zhu, R., Song, C., Yang, J., Yi, Q., Li, B., & Liu, S. (2016). Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy. Molecular Medicine Reports, 14, 2846-2852. https://doi.org/10.3892/mmr.2016.5579
MLA
Zhu, R., Song, C., Yang, J., Yi, Q., Li, B., Liu, S."Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy". Molecular Medicine Reports 14.3 (2016): 2846-2852.
Chicago
Zhu, R., Song, C., Yang, J., Yi, Q., Li, B., Liu, S."Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy". Molecular Medicine Reports 14, no. 3 (2016): 2846-2852. https://doi.org/10.3892/mmr.2016.5579
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