Open Access

Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis

Corrigendum in: /10.3892/mmr.2016.5882

  • Authors:
    • Chao Huang
    • Hong Liu
    • Xiuli Gong
    • Bin Wen
    • Dan Chen
    • Jinyuan Liu
    • Fengliang Hu
  • View Affiliations

  • Published online on: August 1, 2016     https://doi.org/10.3892/mmr.2016.5584
  • Pages: 2555-2565
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to observe the varying expression of biomarkers in the microenvironment adjacent to colorectal cancer lesions to provide additional insight into the functions of microenvironment components in carcinogenesis and present a novel or improved indicator for early diagnosis of cancer. A total of 144 human samples from three different locations in 48 patients were collected, these locations were 10, 5 and 2 cm from the colorectal cancer lesion, respectively. The biomarkers analyzed included E‑cadherin, cytokeratin 18 (CK18), hyaluronidase‑1 (Hyal‑1), collagen type I (Col‑I), Crumbs3 (CRB3), vimentin, proteinase activated receptor 3 (PAR‑3), α‑smooth muscle actin (α‑SMA), cyclin D1 (CD1) and cluster of differentiation (CD)133. In addition, crypt architecture was observed. Related functional analysis of proteins was performed using hierarchical index cluster analysis. More severe destroyed crypt architecture closer to the cancer lesions was observed compared with the 10 cm sites, with certain crypts degraded entirely. Expression levels of E‑cadherin, CK18, CRB3 and PAR‑3 were lower in 2 cm sites compared with the 10 cm sites (all P<0.001), while the expression levels of the other biomarkers in the 2 cm sites were increased compared with 10 cm sites (all P<0.0001). Notably, the expression of CK18 in 2 cm sites was higher than in the 5 cm site (P<0.0001), which was different from the expression of E‑cadherin, CRB3 and PAR‑3. The expression levels of Hyal‑1 and Col‑I at the 2 cm sites were lower than that of the 5 cm sites (P>0.05 and P=0.0001, respectively), while the expression of vimentin, α‑SMA, CD1 and CD133 were not. Hyal‑1 and Col‑I may be independently important in cancer initiation in the tumor microenvironment. The results of the present study suggest that the biomarkers in the tissue microenvironment are associated with early tumorigenesis and may contribute to the development of carcinomas. These observations may be useful for early diagnosis of colorectal cancer.
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September-2016
Volume 14 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Huang C, Liu H, Gong X, Wen B, Chen D, Liu J and Hu F: Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis Corrigendum in /10.3892/mmr.2016.5882. Mol Med Rep 14: 2555-2565, 2016
APA
Huang, C., Liu, H., Gong, X., Wen, B., Chen, D., Liu, J., & Hu, F. (2016). Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis Corrigendum in /10.3892/mmr.2016.5882. Molecular Medicine Reports, 14, 2555-2565. https://doi.org/10.3892/mmr.2016.5584
MLA
Huang, C., Liu, H., Gong, X., Wen, B., Chen, D., Liu, J., Hu, F."Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis Corrigendum in /10.3892/mmr.2016.5882". Molecular Medicine Reports 14.3 (2016): 2555-2565.
Chicago
Huang, C., Liu, H., Gong, X., Wen, B., Chen, D., Liu, J., Hu, F."Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis Corrigendum in /10.3892/mmr.2016.5882". Molecular Medicine Reports 14, no. 3 (2016): 2555-2565. https://doi.org/10.3892/mmr.2016.5584