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The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia

  • Authors:
    • Baoguo Sun
    • Haoxuan Luo
    • Liuxiang Deng
    • Shijun Zhang
    • Zexiong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine, First Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3113-3121
    |
    Published online on: August 8, 2016
       https://doi.org/10.3892/mmr.2016.5602
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Abstract

Various studies have investigated hepatic carcinoma cachexia, however, there is little published information regarding the effect of Chinese Medicine carcinoma cachexia. The present study was performed to investigate the effect of modified Chinese herbal compound jianpijiedu (MJPJD) on a mouse model of ascites‑induced hepatic carcinoma cachexia. C57BL/6 mice were randomized to five groups: Control (Group A); xenograft tumor (Group B); low concentration of MJPJD (Group C); high concentration of MJPJD (Group D) and medroxyprogesterone (MPA) combined with indometacin (IND; Group E). The mouse model of ascites‑induced hepatic carcinoma cachexia was established by abdominal injection of H22 hepatic carcinoma cells. Subsequently, the body weight, food intake and gastrocnemius weight were recorded, and the levels of interleukin (IL)‑lα, IL‑6, tumor necrosis factor‑α (TNF‑α) in ascites were detected by enzyme‑linked immunosorbent assay. The protein expression levels of muscle RING‑finger protein‑1 (MU‑RF1) and atrogin 1 were detected by western blotting and immunohistochemistry, and the mRNA levels in gastrocnemius were detected by reverse transcription‑quantitative polymerase chain reaction. Compared with the xenograft tumor group, the administration of MJPJD inhibited the increase in body weight and the volume of ascites, the consumption of gastrocnemius was reduced, the net weight of ascites was maintained, the food intake was enhanced and the levels of the cytokines IL‑lα, IL‑6, TNF‑α in ascites and the levels of MU‑RF1 and atrogin 1 proteins were reduced. These results indicated that MJPJD delays the pathological process of ascites‑induced hepatic carcinoma cachexia, and the mechanism of action may be correlated with a reduction in the levels of IL‑lα, IL‑6, TNF‑α and inhibiting the activation of the ubiquitin proteosome pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Sun B, Luo H, Deng L, Zhang S and Chen Z: The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia. Mol Med Rep 14: 3113-3121, 2016.
APA
Sun, B., Luo, H., Deng, L., Zhang, S., & Chen, Z. (2016). The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia. Molecular Medicine Reports, 14, 3113-3121. https://doi.org/10.3892/mmr.2016.5602
MLA
Sun, B., Luo, H., Deng, L., Zhang, S., Chen, Z."The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia". Molecular Medicine Reports 14.4 (2016): 3113-3121.
Chicago
Sun, B., Luo, H., Deng, L., Zhang, S., Chen, Z."The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia". Molecular Medicine Reports 14, no. 4 (2016): 3113-3121. https://doi.org/10.3892/mmr.2016.5602
Copy and paste a formatted citation
x
Spandidos Publications style
Sun B, Luo H, Deng L, Zhang S and Chen Z: The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia. Mol Med Rep 14: 3113-3121, 2016.
APA
Sun, B., Luo, H., Deng, L., Zhang, S., & Chen, Z. (2016). The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia. Molecular Medicine Reports, 14, 3113-3121. https://doi.org/10.3892/mmr.2016.5602
MLA
Sun, B., Luo, H., Deng, L., Zhang, S., Chen, Z."The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia". Molecular Medicine Reports 14.4 (2016): 3113-3121.
Chicago
Sun, B., Luo, H., Deng, L., Zhang, S., Chen, Z."The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia". Molecular Medicine Reports 14, no. 4 (2016): 3113-3121. https://doi.org/10.3892/mmr.2016.5602
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