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October-2016 Volume 14 Issue 4

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Article

PRDM16 is associated with evasion of apoptosis by prostatic cancer cells according to RNA interference screening

  • Authors:
    • Shaoxing Zhu
    • Yipeng Xu
    • Mei Song
    • Guiping Chen
    • Hua Wang
    • Yang Zhao
    • Zongping Wang
    • Fangyin Li
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China, Department of Ultrasonography, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China
  • Pages: 3357-3361
    |
    Published online on: August 8, 2016
       https://doi.org/10.3892/mmr.2016.5605
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Abstract

Histone methylation, which is regulated by histone methyltransferases (HMTs) and histone demethylases (HDMs), has been indicated to be involved in a variety of diseases, particularly in cancer, including androgen‑independent prostate cancer (PCa). However, the functions of HMTs and HDTs in cancer have largely remained elusive. The present study, utilized an RNA interference screening using a lentiviral small hairpin (sh)RNA library to systematically elucidate the function of HMTs and HDTs in PCa cell growth and viability. Nine HMTs and HDTs, namely FBXO11, PRDM10, JMJD8, MLL, SETD4, JMJD7, PRMT2, MEN1 and PRDM16, were identified to affect DU145 cell viability, as indicated by an MTS assay subsequent to knockdown of the specific genes using shRNA pools. Furthermore, flow cytometric analysis and western blot analysis of apoptosis‑associated proteins indicated that PRDM16 has an anti‑apoptotic role in PCa cells. In addition, the spliced form, sPRDM16/MEL1S, was detected to be overexpressed in PCa cell lines. In conclusion, the present study indicated an important oncogenic role of sPRDM16/MEL1S in PCa and suggested that PRDM16 may represent a novel therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu S, Xu Y, Song M, Chen G, Wang H, Zhao Y, Wang Z and Li F: PRDM16 is associated with evasion of apoptosis by prostatic cancer cells according to RNA interference screening. Mol Med Rep 14: 3357-3361, 2016.
APA
Zhu, S., Xu, Y., Song, M., Chen, G., Wang, H., Zhao, Y. ... Li, F. (2016). PRDM16 is associated with evasion of apoptosis by prostatic cancer cells according to RNA interference screening. Molecular Medicine Reports, 14, 3357-3361. https://doi.org/10.3892/mmr.2016.5605
MLA
Zhu, S., Xu, Y., Song, M., Chen, G., Wang, H., Zhao, Y., Wang, Z., Li, F."PRDM16 is associated with evasion of apoptosis by prostatic cancer cells according to RNA interference screening". Molecular Medicine Reports 14.4 (2016): 3357-3361.
Chicago
Zhu, S., Xu, Y., Song, M., Chen, G., Wang, H., Zhao, Y., Wang, Z., Li, F."PRDM16 is associated with evasion of apoptosis by prostatic cancer cells according to RNA interference screening". Molecular Medicine Reports 14, no. 4 (2016): 3357-3361. https://doi.org/10.3892/mmr.2016.5605
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu S, Xu Y, Song M, Chen G, Wang H, Zhao Y, Wang Z and Li F: PRDM16 is associated with evasion of apoptosis by prostatic cancer cells according to RNA interference screening. Mol Med Rep 14: 3357-3361, 2016.
APA
Zhu, S., Xu, Y., Song, M., Chen, G., Wang, H., Zhao, Y. ... Li, F. (2016). PRDM16 is associated with evasion of apoptosis by prostatic cancer cells according to RNA interference screening. Molecular Medicine Reports, 14, 3357-3361. https://doi.org/10.3892/mmr.2016.5605
MLA
Zhu, S., Xu, Y., Song, M., Chen, G., Wang, H., Zhao, Y., Wang, Z., Li, F."PRDM16 is associated with evasion of apoptosis by prostatic cancer cells according to RNA interference screening". Molecular Medicine Reports 14.4 (2016): 3357-3361.
Chicago
Zhu, S., Xu, Y., Song, M., Chen, G., Wang, H., Zhao, Y., Wang, Z., Li, F."PRDM16 is associated with evasion of apoptosis by prostatic cancer cells according to RNA interference screening". Molecular Medicine Reports 14, no. 4 (2016): 3357-3361. https://doi.org/10.3892/mmr.2016.5605
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