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Article

Identification of microRNA profiles associated with refractory primary biliary cirrhosis

  • Authors:
    • Teppei Sakamoto
    • Asahiro Morishita
    • Takako Nomura
    • Joji Tani
    • Hisaaki Miyoshi
    • Hirohiro Yoneyama
    • Hisakazu Iwama
    • Takashi Himoto
    • Tsutomu Masaki
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology and Neurology, Kagawa University Faculty of Medicine, Miki‑cho, Kagawa 761‑0793, Japan, Life Science Research Center, Kagawa University Faculty of Medicine, Miki‑cho, Kagawa 761‑0793, Japan, Department of Medical Technology, Kagawa Prefectural University of Health Sciences, Takamatsu, Kagawa 761‑0123, Japan
  • Pages: 3350-3356
    |
    Published online on: August 9, 2016
       https://doi.org/10.3892/mmr.2016.5606
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Abstract

MicroRNAs (miRNAs) are small, endogenous, non-coding RNAs that control the target gene translation by RNA interference; miRNAs are associated with cellular processes, including proliferation, differentiation, apoptosis, and cell survival. Primary biliary cirrhosis (PBC) is a chronic cholestatic liver disease of unknown etiology. One third of patients with PBC demonstrate suboptimal responses, which result in worse outcomes. It has been previously reported that miRNAs are involved in drug resistance, however, the association between miRNA expression levels and refractory PBC remains to be fully elucidated. In the present study, among the 20 patients with PBC treated with ursodeoxycholic acid or bezafibrate, 15 patients were classed as treatment‑effective, and 5 were classed as being treatment‑resistant. Using the miRNA array technique, miRNA profiles were identified for each group. A total of 35 miRNAs were significantly upregulated, and 23 were significantly downregulated in the treatment‑resistant group compared with the treatment‑effective group. In order to examine the association between the highly altered miRNAs and clinical features of the two groups, numerous parameters were analyzed. Elevated levels of direct bilirubin, aspartate transaminase (AST), and alanine transaminase (ALT) were identified to be associated with miRNA‑122 upregulation. AST, ALT, and γ guanosine triphosphate were additionally associated with miRNA‑378f upregulation. However, the reduction of miRNA‑4311 was associated with reduced levels of AST and ALT. miRNA‑4714‑3p was also negatively correlated with total bilirubin and lactate dehydrogenase. Therefore, identifying the miRNA profile was demonstrated to be a useful approach in the characterization of PBC development. It is suggested that highly altered miRNAs may be potential biomarkers for use in the development of treatment of patients with refractory PBC.
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Copy and paste a formatted citation
Spandidos Publications style
Sakamoto T, Morishita A, Nomura T, Tani J, Miyoshi H, Yoneyama H, Iwama H, Himoto T and Masaki T: Identification of microRNA profiles associated with refractory primary biliary cirrhosis. Mol Med Rep 14: 3350-3356, 2016.
APA
Sakamoto, T., Morishita, A., Nomura, T., Tani, J., Miyoshi, H., Yoneyama, H. ... Masaki, T. (2016). Identification of microRNA profiles associated with refractory primary biliary cirrhosis. Molecular Medicine Reports, 14, 3350-3356. https://doi.org/10.3892/mmr.2016.5606
MLA
Sakamoto, T., Morishita, A., Nomura, T., Tani, J., Miyoshi, H., Yoneyama, H., Iwama, H., Himoto, T., Masaki, T."Identification of microRNA profiles associated with refractory primary biliary cirrhosis". Molecular Medicine Reports 14.4 (2016): 3350-3356.
Chicago
Sakamoto, T., Morishita, A., Nomura, T., Tani, J., Miyoshi, H., Yoneyama, H., Iwama, H., Himoto, T., Masaki, T."Identification of microRNA profiles associated with refractory primary biliary cirrhosis". Molecular Medicine Reports 14, no. 4 (2016): 3350-3356. https://doi.org/10.3892/mmr.2016.5606
Copy and paste a formatted citation
x
Spandidos Publications style
Sakamoto T, Morishita A, Nomura T, Tani J, Miyoshi H, Yoneyama H, Iwama H, Himoto T and Masaki T: Identification of microRNA profiles associated with refractory primary biliary cirrhosis. Mol Med Rep 14: 3350-3356, 2016.
APA
Sakamoto, T., Morishita, A., Nomura, T., Tani, J., Miyoshi, H., Yoneyama, H. ... Masaki, T. (2016). Identification of microRNA profiles associated with refractory primary biliary cirrhosis. Molecular Medicine Reports, 14, 3350-3356. https://doi.org/10.3892/mmr.2016.5606
MLA
Sakamoto, T., Morishita, A., Nomura, T., Tani, J., Miyoshi, H., Yoneyama, H., Iwama, H., Himoto, T., Masaki, T."Identification of microRNA profiles associated with refractory primary biliary cirrhosis". Molecular Medicine Reports 14.4 (2016): 3350-3356.
Chicago
Sakamoto, T., Morishita, A., Nomura, T., Tani, J., Miyoshi, H., Yoneyama, H., Iwama, H., Himoto, T., Masaki, T."Identification of microRNA profiles associated with refractory primary biliary cirrhosis". Molecular Medicine Reports 14, no. 4 (2016): 3350-3356. https://doi.org/10.3892/mmr.2016.5606
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