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MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma

  • Authors:
    • Li Qian
    • Longlong Lin
    • Yufeng Du
    • Xiaoyan Hao
    • Yuze Zhao
    • Xuejun Liu
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200032, P.R. China
    Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3021-3028
    |
    Published online on: August 19, 2016
       https://doi.org/10.3892/mmr.2016.5643
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Abstract

MicroRNAs (miRNAs) have been demonstrated to be critical in regulating tumor development and progression. The present study investigated the expression of miR‑588 using reverse transcription‑quantitative polymerase chain reaction analysis in 85 cases of lung squamous cell carcinoma (SCC), and observed the correlation between the expression of miR‑588 with clinical pathologic features. The results indicated that the expression of miR‑588 was predominantly lower in the tumor samples, compared with non‑tumorous samples, and was negatively associated with tumor stages and lymph node invasion. The present study also examined the significance of the expression of miR‑588 in SCC using gain‑ and loss‑of‑function analyses. It was found that miR‑588 inhibited tumor cell migration and invasion. In addition, it was revealed that the overexpression of miR‑588 in SCC cells reduced the mRNA and protein levels of progranulin (GRN), whereas miR‑588 silencing increased the expression of GRN. A luciferase activity assay showed that miR‑588 was able to directly bind to the 3'untranslated region of GRN and regulate its expression. Furthermore, it was found that the expression of GRN was inversely correlated with the expression of miR‑588 in 85 paired SCC samples. These results indicated that GRN was involved in the miR-588-mediated suppressive functions in the progression of SCC.
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Copy and paste a formatted citation
Spandidos Publications style
Qian L, Lin L, Du Y, Hao X, Zhao Y and Liu X: MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma. Mol Med Rep 14: 3021-3028, 2016.
APA
Qian, L., Lin, L., Du, Y., Hao, X., Zhao, Y., & Liu, X. (2016). MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma. Molecular Medicine Reports, 14, 3021-3028. https://doi.org/10.3892/mmr.2016.5643
MLA
Qian, L., Lin, L., Du, Y., Hao, X., Zhao, Y., Liu, X."MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma". Molecular Medicine Reports 14.4 (2016): 3021-3028.
Chicago
Qian, L., Lin, L., Du, Y., Hao, X., Zhao, Y., Liu, X."MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma". Molecular Medicine Reports 14, no. 4 (2016): 3021-3028. https://doi.org/10.3892/mmr.2016.5643
Copy and paste a formatted citation
x
Spandidos Publications style
Qian L, Lin L, Du Y, Hao X, Zhao Y and Liu X: MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma. Mol Med Rep 14: 3021-3028, 2016.
APA
Qian, L., Lin, L., Du, Y., Hao, X., Zhao, Y., & Liu, X. (2016). MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma. Molecular Medicine Reports, 14, 3021-3028. https://doi.org/10.3892/mmr.2016.5643
MLA
Qian, L., Lin, L., Du, Y., Hao, X., Zhao, Y., Liu, X."MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma". Molecular Medicine Reports 14.4 (2016): 3021-3028.
Chicago
Qian, L., Lin, L., Du, Y., Hao, X., Zhao, Y., Liu, X."MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma". Molecular Medicine Reports 14, no. 4 (2016): 3021-3028. https://doi.org/10.3892/mmr.2016.5643
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