Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
October-2016 Volume 14 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2016 Volume 14 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Expression of early and late cellular damage markers by ARPE-19 cells following prolonged treatment with UV-A radiation

  • Authors:
    • Giuseppe Tringali
    • Beatrice Sampaolese
    • Maria Elisabetta Clementi
  • View Affiliations / Copyright

    Affiliations: Institute of Pharmacology, Catholic University Medical School, I‑00168 Rome, Italy, CNR‑ICRM Institute of ‘Chimica del Riconoscimento Molecolare’, c/o Institute of Biochemistry and Clinical Biochemistry, Catholic University Medical School, I‑00168 Rome, Italy
  • Pages: 3485-3489
    |
    Published online on: August 19, 2016
       https://doi.org/10.3892/mmr.2016.5649
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Pathological alterations to the retinal pigment epithelium underlie several eye diseases, which lead to visual impairment and even blindness. Exposure to ultraviolet (UV) radiation is associated with some skin and ocular pathologies; UV radiation may induce DNA breakdown and cause cellular damage through the production of reactive oxygen species (ROS), thus leading to programmed cell death. The present study aimed to investigate the production of ROS and the gene expression levels of anti‑ and proapoptotic proteins [B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax) and caspase‑3] in human retinal pigment epithelial cells (ARPE‑19) treated with UV‑A for 5 h consecutively. The results demonstrated that prolonged exposure to UV‑A induced: i) Cell death, the decrease in cell viability was time‑dependent and reached statistical significance after 3 h; ii) a significant and substantial increase in ROS levels that remained constant for the duration of the experiment, the levels were significantly increased after 1 h of exposure; iii) an activation of apoptotic genes (Bax and caspase‑3) after 1 h of treatment, which was accompanied by a decrease in the anti‑apoptotic gene Bcl‑2; and iv) a loss of apoptotic signals and a rapid decrease in cellular viability after 3 h of consecutive treatment. These processes may trigger necrosis, which was observed in the cells following treatment with UV‑A for 5 consecutive hours. In conclusion, the present study is the first, to the best of our knowledge, to provide in vitro evidence regarding the sequence of events that underlie the cellular damage induced by prolonged UV‑A radiation, starting from the first 30 min of treatment. UV‑A radiation resulted in the activation of apoptotic events, and subsequently led to irreversible cell necrosis.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Kuznetsova AV, Kurinov AM and Aleksandrova MA: Cell models to study regulation of cell transformation in pathologies of retinal pigment epithelium. J Ophthalmol. 2014:8017872014. View Article : Google Scholar : PubMed/NCBI

2 

Rizzolo LJ: Barrier properties of cultured retinal pigment epithelium. Exp Eye Res. 126:16–26. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Pfeffer BA and Philp NJ: Cell culture of retinal pigment epithelium: Special Issue. Exp Eye Res. 126:1–4. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Glickman RD: Ultraviolet phototoxicity to the retina. Eye Contact Lens. 37:196–205. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Blasiak J, Petrovski G, Veréb Z, Facskó A and Kaarniranta K: Oxidative stress, hypoxia, and autophagy in the neovascular processes of age-related macular degeneration. Biomed Res Int. 2014:7680262014. View Article : Google Scholar : PubMed/NCBI

6 

Tu G, Zhang YF, Wei W, Li L, Zhang Y, Yang J and Xing Y: Allicin attenuates H2O2-induced cytotoxicity in retinal pigmented epithelial cells by regulating the levels of reactive oxygen species. Mol Med Rep. 13:2320–2326. 2016.PubMed/NCBI

7 

Plestina-Borjan I and Klinger-Lasić M: Long-term exposure to solar ultraviolet radiation as a risk factor for age-related macular degeneration. Coll Antropol. 31(Suppl 1): 33–38. 2007.PubMed/NCBI

8 

Li X, Zhao H, Wang Q, Liang H and Jiang X: Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway. Mol Med Rep. 11:3746–3752. 2015.PubMed/NCBI

9 

Øsnes-Ringen O, Azqueta AO, Moe MC, Zetterström C, Røger M, Nicolaissen B and Collins AR: DNA damage in lens epithelium of cataract patients in vivo and ex vivo. Acta Ophthalmol. 91:652–656. 2013. View Article : Google Scholar

10 

Ji Y, Cai L, Zheng T, Ye H, Rong X, Rao J and Lu Y: The mechanism of UVB irradiation induced-apoptosis in cataract. Mol Cell Biochem. 401:87–95. 2015. View Article : Google Scholar

11 

Roduit R and Schorderet DF: MAP kinase pathways in UV-induced apoptosis of retinal pigment epithelium ARPE19 cells. Apoptosis. 13:343–353. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Hanus J, Zhang H, Wang Z, Liu Q, Zhou Q and Wang S: Induction of necrotic cell death by oxidative stress in retinal pigment epithelial cells. Cell Death Dis. 4:e9652013. View Article : Google Scholar : PubMed/NCBI

13 

Shah D, Naciri M, Clee P and Al-Rubeai M: NucleoCounter-An efficient technique for the determination of cell number and viability in animal cell culture processes. Cytotechnology. 51:39–44. 2006. View Article : Google Scholar

14 

Chan CM, Huang CH, Li HJ, Hsiao CY, Su CC, Lee PL and Hung CF: Protective effects of resveratrol against UVA-induced damage in ARPE19 cells. Int J Mol Sci. 16:5789–5802. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Murakami Y, Matsumoto H, Roh M, Giani A, Kataoka K, Morizane Y, Kayama M, Thanos A, Nakatake S, Notomi S, et al: Programmed necrosis, not apoptosis, is a key mediator of cell loss and DAMP-mediated inflammation in dsRNA-induced retinal degeneration. Cell Death Differ. 21:270–277. 2014. View Article : Google Scholar :

16 

Hildeman DA, Mitchell T, Aronow B, Wojciechowski S, Kappler J and Marrack P: Control of Bcl-2 expression by reactive oxygen species. Proc Natl Acad Sci USA. 100:15035–15040. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Han S, Lu Q and Wang N: Apr3 accelerates the senescence of human retinal pigment epithelial cells. Mol Med Rep. 13:3121–3126. 2016.PubMed/NCBI

18 

Kannan K and Jain SK: Oxidative stress and apoptosis. Pathophysiology. 7:153–163. 2000. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Tringali G, Sampaolese B and Clementi ME: Expression of early and late cellular damage markers by ARPE-19 cells following prolonged treatment with UV-A radiation. Mol Med Rep 14: 3485-3489, 2016.
APA
Tringali, G., Sampaolese, B., & Clementi, M.E. (2016). Expression of early and late cellular damage markers by ARPE-19 cells following prolonged treatment with UV-A radiation. Molecular Medicine Reports, 14, 3485-3489. https://doi.org/10.3892/mmr.2016.5649
MLA
Tringali, G., Sampaolese, B., Clementi, M. E."Expression of early and late cellular damage markers by ARPE-19 cells following prolonged treatment with UV-A radiation". Molecular Medicine Reports 14.4 (2016): 3485-3489.
Chicago
Tringali, G., Sampaolese, B., Clementi, M. E."Expression of early and late cellular damage markers by ARPE-19 cells following prolonged treatment with UV-A radiation". Molecular Medicine Reports 14, no. 4 (2016): 3485-3489. https://doi.org/10.3892/mmr.2016.5649
Copy and paste a formatted citation
x
Spandidos Publications style
Tringali G, Sampaolese B and Clementi ME: Expression of early and late cellular damage markers by ARPE-19 cells following prolonged treatment with UV-A radiation. Mol Med Rep 14: 3485-3489, 2016.
APA
Tringali, G., Sampaolese, B., & Clementi, M.E. (2016). Expression of early and late cellular damage markers by ARPE-19 cells following prolonged treatment with UV-A radiation. Molecular Medicine Reports, 14, 3485-3489. https://doi.org/10.3892/mmr.2016.5649
MLA
Tringali, G., Sampaolese, B., Clementi, M. E."Expression of early and late cellular damage markers by ARPE-19 cells following prolonged treatment with UV-A radiation". Molecular Medicine Reports 14.4 (2016): 3485-3489.
Chicago
Tringali, G., Sampaolese, B., Clementi, M. E."Expression of early and late cellular damage markers by ARPE-19 cells following prolonged treatment with UV-A radiation". Molecular Medicine Reports 14, no. 4 (2016): 3485-3489. https://doi.org/10.3892/mmr.2016.5649
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team