Open Access

Allicin protects traumatic spinal cord injury through regulating the HSP70/Akt/iNOS pathway in mice

  • Authors:
    • Shunyi Wang
    • Dongliang Ren
  • View Affiliations

  • Published online on: August 19, 2016     https://doi.org/10.3892/mmr.2016.5651
  • Pages: 3086-3092
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Allicin is a major component of garlic, extracted as an oily liquid. The present study was designed to investigate the beneficial effects of allicin on traumatic spinal cord injury (TSCI) in mice, and whether the effects are mediated via regulation of the heat shock protein 70 (HSP70), v‑akt murine thymoma viral oncogene homolog 1 (Akt) and inducible nitric oxide synthase (iNOS) pathways. Adult BALB/c mice (30‑40 g) received a laminectomy at the T9 vertebral level as a model of TSCI. In the present study, treatment of the TSCI mice with allicin significantly increased their Basso, Beattie and Bresnahan (BBB) scores (P<0.01) and reduced the spinal cord water content (P<0.01). This protective effect was associated with the inhibition of oxidative stress and inflammatory responses in TSCI mice. Western blot analysis demonstrated that allicin increased the protein levels of HSP70, increased the phosphorylation of Akt and reduced the iNOS protein expression levels in TSCI mice. Additionally, treatment with allicin significantly reduced the levels of ROS and enhanced the NADH levels in TSCI mice. Collectively, these data demonstrate that the effects of allicin on TSCI are mediated via regulation of the HSP70, Akt and iNOS pathways in mice.
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October-2016
Volume 14 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang S and Wang S: Allicin protects traumatic spinal cord injury through regulating the HSP70/Akt/iNOS pathway in mice. Mol Med Rep 14: 3086-3092, 2016
APA
Wang, S., & Wang, S. (2016). Allicin protects traumatic spinal cord injury through regulating the HSP70/Akt/iNOS pathway in mice. Molecular Medicine Reports, 14, 3086-3092. https://doi.org/10.3892/mmr.2016.5651
MLA
Wang, S., Ren, D."Allicin protects traumatic spinal cord injury through regulating the HSP70/Akt/iNOS pathway in mice". Molecular Medicine Reports 14.4 (2016): 3086-3092.
Chicago
Wang, S., Ren, D."Allicin protects traumatic spinal cord injury through regulating the HSP70/Akt/iNOS pathway in mice". Molecular Medicine Reports 14, no. 4 (2016): 3086-3092. https://doi.org/10.3892/mmr.2016.5651