Anti-wrinkle effects of Sargassum muticum ethyl acetate fraction on ultraviolet B-irradiated hairless mouse skin and mechanistic evaluation in the human HaCaT keratinocyte cell line

Song,Jae  Hyoung; Piao,Mei  Jing; Han,Xia; Kang,Kyoung  Ah; Kang,Hee  Kyoung; Yoon,Weon  Jong; Ko,Mi  Hee; Lee,Nam  Ho; Lee,Mi  Young; Chae,Sungwook; Hyun,Jin  Won

doi:10.3892/mmr.2016.5655

Anti-wrinkle effects of Sargassum muticum ethyl acetate fraction on ultraviolet B-irradiated hairless mouse skin and mechanistic evaluation in the human HaCaT keratinocyte cell line

Authors:
- Jae Hyoung Song
- Mei Jing Piao
- Xia Han
- Kyoung Ah Kang
- Hee Kyoung Kang
- Weon Jong Yoon
- Mi Hee Ko
- Nam Ho Lee
- Mi Young Lee
- Sungwook Chae
- Jin Won Hyun
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Affiliations: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea, Jeju Biodiversity Research Institute, Jeju High‑Tech Development Institute, Jeju 63612, Republic of Korea, Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju 63243, Republic of Korea
Published online on: August 19, 2016 https://doi.org/10.3892/mmr.2016.5655
Pages: 2937-2944
Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the photoprotective properties of the ethyl acetate fraction of Sargassum muticum (SME) against ultraviolet B (UVB)‑induced skin damage and photoaging in a mouse model. HR‑1 strain hairless male mice were divided into three groups: An untreated control group, a UVB‑irradiated vehicle group and a UVB‑irradiated SME group. The UVB‑irradiated mice in the SME group were orally administered with SME (100 mg/kg body weight in 0.1 ml water per day) and then exposed to radiation at a dose of 60‑120 mJ/cm2. Wrinkle formation and skin damage were evaluated by analysis of skin replicas, epidermal thickness and collagen fiber integrity in the dermal connective tissue. The mechanism underlying the action of SME was also investigated in the human HaCaT keratinocyte cell line following exposure of the cells to UVB at a dose of 30 mJ/cm2. The protein expression levels and activity of matrix metalloproteinase‑1 (MMP‑1), and the binding of activator protein‑1 (AP‑1) to the MMP‑1 promoter were assessed in the HaCaT cells using western blot analysis, an MMP‑1 fluorescent assay and a chromatin immune‑precipitation assay, respectively. The results showed that the mean length and depth of the wrinkles in the UVB‑exposed hairless mice were significantly improved by oral administration of SME, which also prevented the increase in epidermal thickness triggered by UVB irradiation. Furthermore, a marked increase in collagen bundle formation was observed in the UVB‑treated mice with SME administration. SME pretreatment also significantly inhibited the UVB‑induced upregulation in the expression and activity of MMP‑1 in the cultured HaCaT keratinocytes, and the UVB‑enhanced association of AP‑1 with the MMP‑1 promoter. These results suggested that SME may be useful as an anti-photoaging resource for the skin.

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