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Article

Knockdown of TACC3 inhibits trophoblast cell migration and invasion through the PI3K/Akt signaling pathway

  • Authors:
    • Xiaojun Zhu
    • Qianqian Cao
    • Xia Li
    • Zhengping Wang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China
  • Pages: 3437-3442
    |
    Published online on: August 19, 2016
       https://doi.org/10.3892/mmr.2016.5659
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Abstract

The insufficient invasion of trophoblasts is known to be correlated with the development of preeclampsia. Transforming acidic coiled‑coil protein 3 (TACC3), a member of the TACC domain family, is important in the regulation of cell differentiation, migration and invasion. However, the role of TACC3 in trophoblast function during placental development remains to be fully elucidated. The present study aimed to determine the expression and function of TACC3 in human placenta and to examine the underlying mechanisms. TACC3 expression was analyzed in preeclamptic placentas using reverse transcription‑quantitative polymerase chain reaction and western blotting. Cell proliferation was determined by the MTT assay, and cell migration and invasion were measured using Transwell assays. The expression levels of TACC3, matrix metalloproteinase (MMP)‑2, MMP‑9, tissue inhibitor of metalloproteinase (TIMP)‑1, TIMP‑2, phosphoinositide 3‑kinase (PI3K), phosphorylated (p)‑PI3K, AKT and p‑AKT were detected by western blotting. The results showed that the expression of TACC3 was downregulated in preeclamptic placentas. The knockdown of TACC3 significantly inhibited HTR8/SVneo cell proliferation, migration and invasion, and inhibited the expression of matrix metalloproteinases. In addition, the knockdown of TACC3 significantly reduced the levels of p‑PI3K and Akt in the HTR8/SVneo cells. Taken together, the results of the present study demonstrated that the knockdown of TACC3 inhibited the migration and invasion of HTR8/SVneo cells through suppression of the PI3K/Akt signaling pathway. Therefore, TACC3 may serve as a novel potential target for treating preeclampsia.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu X, Cao Q, Li X and Wang Z: Knockdown of TACC3 inhibits trophoblast cell migration and invasion through the PI3K/Akt signaling pathway. Mol Med Rep 14: 3437-3442, 2016.
APA
Zhu, X., Cao, Q., Li, X., & Wang, Z. (2016). Knockdown of TACC3 inhibits trophoblast cell migration and invasion through the PI3K/Akt signaling pathway. Molecular Medicine Reports, 14, 3437-3442. https://doi.org/10.3892/mmr.2016.5659
MLA
Zhu, X., Cao, Q., Li, X., Wang, Z."Knockdown of TACC3 inhibits trophoblast cell migration and invasion through the PI3K/Akt signaling pathway". Molecular Medicine Reports 14.4 (2016): 3437-3442.
Chicago
Zhu, X., Cao, Q., Li, X., Wang, Z."Knockdown of TACC3 inhibits trophoblast cell migration and invasion through the PI3K/Akt signaling pathway". Molecular Medicine Reports 14, no. 4 (2016): 3437-3442. https://doi.org/10.3892/mmr.2016.5659
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu X, Cao Q, Li X and Wang Z: Knockdown of TACC3 inhibits trophoblast cell migration and invasion through the PI3K/Akt signaling pathway. Mol Med Rep 14: 3437-3442, 2016.
APA
Zhu, X., Cao, Q., Li, X., & Wang, Z. (2016). Knockdown of TACC3 inhibits trophoblast cell migration and invasion through the PI3K/Akt signaling pathway. Molecular Medicine Reports, 14, 3437-3442. https://doi.org/10.3892/mmr.2016.5659
MLA
Zhu, X., Cao, Q., Li, X., Wang, Z."Knockdown of TACC3 inhibits trophoblast cell migration and invasion through the PI3K/Akt signaling pathway". Molecular Medicine Reports 14.4 (2016): 3437-3442.
Chicago
Zhu, X., Cao, Q., Li, X., Wang, Z."Knockdown of TACC3 inhibits trophoblast cell migration and invasion through the PI3K/Akt signaling pathway". Molecular Medicine Reports 14, no. 4 (2016): 3437-3442. https://doi.org/10.3892/mmr.2016.5659
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