Open Access

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage

  • Authors:
    • Xiaoying Zhang
    • Cuicui Zhu
    • Qiong Luo
    • Jv Dong
    • Lv Liu
    • Min Li
    • Hongtao Zhu
    • Xiangping Ma
    • Jun Wang
  • View Affiliations

  • Published online on: August 24, 2016     https://doi.org/10.3892/mmr.2016.5667
  • Pages: 3595-3601
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the possible damage-repair mechanisms of neural stem cells (NSCs) following hypoxic-ischemic brain damage (HIBD). NSCs obtained from Sprague Dawley rats were treated with tissue homogenate from normal or HIBD tissue, and β‑catenin expression was silenced using siRNA. The differentiation of NSCs was observed by immunofluorescence, and semiquantitative reverse transcription‑polymerase chain reaction and western blot analysis were applied to detect the mRNA and protein expression levels of Ngn1 and BMP4 in the NSCs. Compared with control NSCs, culture with brain tissue homogenate significantly increased the differentiation of NSCs into neurons and oligodendrocytes (P<0.05), whereas differentiation into astrocytes was significantly reduced (P<0.05). Compared with negative control‑transfected cells, knockdown of β‑catenin expression significantly decreased the differentiation of NSCs into neurons and oligodendrocytes (P<0.01), whereas the percentage of NSCs differentiated into astrocytes was significantly increased (P<0.01). Compared with control NSCs, the mRNA and protein expression levels of Ngn1 were significantly increased (P<0.01) and BMP4 levels were significantly reduced (P<0.01) by exposure of the cells to brain tissue homogenate. Compared with the negative control plasmid‑transfected NSCs, the levels of Ngn1 mRNA and protein were significantly reduced by β‑catenin siRNA (P<0.01), whereas BMP4 levels were significantly increased (P<0.01). In summary, the damaged brain tissues in HIBD may promote NSCs to differentiate into neurons for self‑repair processes. β‑Catenin, BMP4 and Ngn1 may be important for the coordination of NSC proliferation and differentiation following HIBD.
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October-2016
Volume 14 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang X, Zhu C, Luo Q, Dong J, Liu L, Li M, Zhu H, Ma X and Wang J: Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage. Mol Med Rep 14: 3595-3601, 2016
APA
Zhang, X., Zhu, C., Luo, Q., Dong, J., Liu, L., Li, M. ... Wang, J. (2016). Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage. Molecular Medicine Reports, 14, 3595-3601. https://doi.org/10.3892/mmr.2016.5667
MLA
Zhang, X., Zhu, C., Luo, Q., Dong, J., Liu, L., Li, M., Zhu, H., Ma, X., Wang, J."Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage". Molecular Medicine Reports 14.4 (2016): 3595-3601.
Chicago
Zhang, X., Zhu, C., Luo, Q., Dong, J., Liu, L., Li, M., Zhu, H., Ma, X., Wang, J."Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage". Molecular Medicine Reports 14, no. 4 (2016): 3595-3601. https://doi.org/10.3892/mmr.2016.5667