Effects of sphingosylphosphorylcholine on cryopreserved fat tissue graft survival

Bae,Yong Chan; Choi,Chi Won; Nam,Kyeong Wook; Song,Ji Sun; Lee,Jae Woo

doi:10.3892/mmr.2016.5677

October-2016 Volume 14 Issue 4

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October-2016 Volume 14 Issue 4

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Article

Effects of sphingosylphosphorylcholine on cryopreserved fat tissue graft survival

Authors:
- Yong Chan Bae
- Chi Won Choi
- Kyeong Wook Nam
- Ji Sun Song
- Jae Woo Lee
View Affiliations / Copyright

Affiliations: Department of Plastic and Reconstructive Surgery, School of Medicine, Pusan National University, Busan 602‑739, Republic of Korea, Biomedical Research Institute, Pusan National University Hospital, Busan 602‑739, Republic of Korea
Pages: 3719-3724
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Published online on: August 26, 2016

https://doi.org/10.3892/mmr.2016.5677
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Abstract

Autogenous microfat grafting is widely used to augment depressed deformities or for other cosmetic purposes. Since the microfat survival rate is unpredictable due to absorption and calcification, previously cryopreserved fat is widely used for secondary procedures. Sphingosylphosphorylcholine (SPC) is a lysophospholipid, which has a role in several cellular responses, and is known to stimulate DNA synthesis and proliferation. Since endothelial progenitor cells (EPCs) are known to enhance the survival rate of transplanted fat tissue, the present study assessed the effects of SPC on EPCs, in order to verify its positive effects on proliferation. Cryopreserved human fat tissues mixed with various concentrations of SPC were grafted into the nude mouse model. After grafting, the viability of each SPC mixed group was determined and compared with that of the non‑SPC group. SPC exhibited a positive effect on EPC proliferation and angiogenic potential over 3 days when used at specific concentrations. The fat grafts of the 3 µM SPC‑treated group weighed significantly more and the volume was markedly increased, as compared with the control group. A reverse transcription‑quantitative polymerase chain reaction was conducted on the total RNA extracted from SPC‑treated fat tissues, which detected increased mRNA expression levels of matrix metallopeptidase‑9 and tumor necrosis factor‑α compared with in the control group. These results indicate that specific concentrations of SPC may exert favorable effects on grafted cryopreserved human fat tissue, which may be due to the increased mRNA expression levels of genes associated with angiogenesis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of sphingosylphosphorylcholine on cryopreserved fat tissue graft survival

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