In vitro antitumor activity of the ethyl acetate extract of Potentilla chinensis in osteosarcoma cancer cells Retraction in /10.3892/mmr.2021.12129

Wan,Guang; Tao,Jin‑Gang; Wang,Guo‑Dong; Liu,Shen‑Peng; Zhao,Hong‑Xing; Liang,Qiu‑Dong

doi:10.3892/mmr.2016.5679

October-2016 Volume 14 Issue 4

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October-2016 Volume 14 Issue 4

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In vitro antitumor activity of the ethyl acetate extract of Potentilla chinensis in osteosarcoma cancer cells

Retraction in: /10.3892/mmr.2021.12129

Authors:
- Guang Wan
- Jin‑Gang Tao
- Guo‑Dong Wang
- Shen‑Peng Liu
- Hong‑Xing Zhao
- Qiu‑Dong Liang
View Affiliations / Copyright

Affiliations: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China

Copyright: © Wan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 3634-3640
|
Published online on: August 26, 2016

https://doi.org/10.3892/mmr.2016.5679
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Abstract

The aim of the current study was to evaluate the anticancer effect of the ethanol extract of Potentilla chinensis, a Chinese medicinal plant. An MTT assay was used to evaluate the cell viability of MG‑63 human osteosarcoma cancer cells and fR‑2 cells. Furthermore, the effect of the extract on apoptosis induction, cell cycle phase distribution and inhibition of cell migration in the MG63 human osteosarcoma cancer cell line was evaluated. The effect of the extract on cell cycle phase distribution was assessed by flow cytometry using propidium iodide (PI). Phase contrast microscopy detected the morphological changes in MG63 cancer cells following extract treatment. The results of the study demonstrated that the extract was cytotoxic to MG63 cancer cells, while the normal cell line (epithelial cell line) showed lower susceptibility. Phase contrast microscopy showed distinguishing morphological features, such as cell shrinkage and blebbing induced by the extract treatment in osteosarcoma cancer cells. The average proportion of Annexin V‑positive cells (total apoptotic cells) significantly increased from 5.6% in the control to 24.2, 38.8 and 55.7% in the 40, 80 and 150 µg/ml groups, respectively. The extract induced early and late apoptosis in the cancer cells. Flow cytometric analysis revealed that the extract induced G0/G1‑cell cycle arrest, which also showed significant dose‑dependence. The extract induced a significant and concentration‑dependent reduction in cell migration. Moreover, DNA fragmentation was also examined by observation of the formation of DNA ladders. It was demonstrated that DNA fragmentation was increased with extract concentration compared with that in the control. Taken together, EEPC may serve as potential therapeutic agent against osteosarcoma, provided that the toxicity profile and in vivo investigations demonstrate that it is safe.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

In vitro antitumor activity of the ethyl acetate extract of Potentilla chinensis in osteosarcoma cancer cells

Retraction in: /10.3892/mmr.2021.12129

This article is mentioned in:

Abstract

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