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Article

Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation

  • Authors:
    • Jing Qin
    • Yongmei Hua
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China
  • Pages: 3871-3877
    |
    Published online on: August 26, 2016
       https://doi.org/10.3892/mmr.2016.5680
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Abstract

Periodontal ligament cells (PDLCs) are important in homeostasis and remodeling in the mechanically‑stimulated periodontium. The aim of the present study was to investigate the effects of hydrogen sulfide (H2S) on periodontal tissue remodeling by examining the mRNA and protein expression levels of alkaline phosphatase (ALP), osteocalcin (OCN) and collagen type I (COL‑1) in human (h)PDLCs induced by tension force application. Cultured hPDLCs were treated with H2S for 24 h, followed by application of a tension force for 1, 3 and 6 h. Cell proliferation and apoptosis were determined using a Cell Counting Kit 8 assay and flow cytometric analysis, respectively. The mRNA expression levels of ALP, OCN and COL‑1 were quantified using reverse transcription‑quantitative polymerase chain reaction analysis, and western blot analysis was used to detect the protein levels of ALP, OCN and COL‑1. The results demonstrated that the mRNA and protein expression levels of ALP, OCN and COL‑1 increased with H2S treatment in a concentration‑dependent manner, which was enhanced by the application of tension force in a relatively short period of time. These findings suggested that H2S may be important in periodontal tissue remodeling during orthodontic tooth movement via increasing hPDLC differentiation, tissue mineralization, bone formation and collagen synthesis.
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Copy and paste a formatted citation
Spandidos Publications style
Qin J and Hua Y: Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation. Mol Med Rep 14: 3871-3877, 2016.
APA
Qin, J., & Hua, Y. (2016). Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation. Molecular Medicine Reports, 14, 3871-3877. https://doi.org/10.3892/mmr.2016.5680
MLA
Qin, J., Hua, Y."Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation". Molecular Medicine Reports 14.4 (2016): 3871-3877.
Chicago
Qin, J., Hua, Y."Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation". Molecular Medicine Reports 14, no. 4 (2016): 3871-3877. https://doi.org/10.3892/mmr.2016.5680
Copy and paste a formatted citation
x
Spandidos Publications style
Qin J and Hua Y: Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation. Mol Med Rep 14: 3871-3877, 2016.
APA
Qin, J., & Hua, Y. (2016). Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation. Molecular Medicine Reports, 14, 3871-3877. https://doi.org/10.3892/mmr.2016.5680
MLA
Qin, J., Hua, Y."Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation". Molecular Medicine Reports 14.4 (2016): 3871-3877.
Chicago
Qin, J., Hua, Y."Effects of hydrogen sulfide on the expression of alkaline phosphatase, osteocalcin and collagen type I in human periodontal ligament cells induced by tension force stimulation". Molecular Medicine Reports 14, no. 4 (2016): 3871-3877. https://doi.org/10.3892/mmr.2016.5680
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