Low mutation percentage of KRAS and BRAF genes in Brazilian anal tumors

  • Authors:
    • Lucas Tadeu Bidinotto
    • Carlos A R Véo
    • Edgar Aleman Loaiza
    • Alessandra Paulino Santos De França
    • Adriana Tarla Lorenzi
    • Luciana Albina Reis Rosa
    • Cristina Mendes De Oliveira
    • José Eduardo Levi
    • Cristovam Scapulatempo‑Neto
    • Adhemar Longatto‑Filho
    • Rui Manuel Reis
  • View Affiliations

  • Published online on: August 26, 2016     https://doi.org/10.3892/mmr.2016.5684
  • Pages: 3791-3797
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Abstract

Anal cancer is a rare type of digestive tract disease, which has had a crescent incidence in a number of regions. Carcinomas are most frequently found, with squamous cell carcinoma (SCC) comprising ~95% of all anal tumors. The major risk factor for development of this type of tumor is human papillomavirus (HPV) infection. However, previous studies have identified patients with anal cancer that are HPV‑/p16‑and observed that they have a poorer outcome compared with HPV+/p16+ patients. This suggests that molecular profile may drive anal cancer progression. The aim of the present study was to evaluate the mutational status of two important oncogenes, KRAS and BRAF, in a series of anal cancer lesions. Resected tumors of the anal canal (n=43) were evaluated, nine of these were high‑grade squamous intra‑epithelial lesion cases (HSIL), 11 were adenocarcinomas, and 23 SCCs. Direct sequencing of KRAS proto‑oncogene, GTPase (KRAS; codons 12 and 13) and B‑Raf proto‑oncogene, serine/threonine kinase (BRAF; codon 600) was performed and associated with patient clinicopathological and molecular features. There was a trend of poorer prognosis of adenocarcinoma compared with HSIL and SCC. Analysis indicated one SCC patient (2.3%) exhibited a KRAS p.G13D mutation, and one adenocarcinoma patient (2.3%) exhibited a BRAF p.V600E mutation. It was observed that, these mutations are rare in anal tumors, and certain patients may be at a disadvantage using targeted therapies based on KRAS and BRAF mutational status. As there is a low mutation percentage in SCCs, adenocarcinomas and HSIL, there may exist other underlying molecular alterations that result in anal cancer development, which require further elucidation.
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October-2016
Volume 14 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Bidinotto LT, Véo CA, Loaiza EA, De França AP, Lorenzi AT, Rosa LA, De Oliveira CM, Levi JE, Scapulatempo‑Neto C, Longatto‑Filho A, Longatto‑Filho A, et al: Low mutation percentage of KRAS and BRAF genes in Brazilian anal tumors. Mol Med Rep 14: 3791-3797, 2016
APA
Bidinotto, L.T., Véo, C.A., Loaiza, E.A., De França, A.P., Lorenzi, A.T., Rosa, L.A. ... Reis, R.M. (2016). Low mutation percentage of KRAS and BRAF genes in Brazilian anal tumors. Molecular Medicine Reports, 14, 3791-3797. https://doi.org/10.3892/mmr.2016.5684
MLA
Bidinotto, L. T., Véo, C. A., Loaiza, E. A., De França, A. P., Lorenzi, A. T., Rosa, L. A., De Oliveira, C. M., Levi, J. E., Scapulatempo‑Neto, C., Longatto‑Filho, A., Reis, R. M."Low mutation percentage of KRAS and BRAF genes in Brazilian anal tumors". Molecular Medicine Reports 14.4 (2016): 3791-3797.
Chicago
Bidinotto, L. T., Véo, C. A., Loaiza, E. A., De França, A. P., Lorenzi, A. T., Rosa, L. A., De Oliveira, C. M., Levi, J. E., Scapulatempo‑Neto, C., Longatto‑Filho, A., Reis, R. M."Low mutation percentage of KRAS and BRAF genes in Brazilian anal tumors". Molecular Medicine Reports 14, no. 4 (2016): 3791-3797. https://doi.org/10.3892/mmr.2016.5684