Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
October-2016 Volume 14 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2016 Volume 14 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea

  • Authors:
    • Yuanxiu Zhu
    • Xiang Ding
    • Mei Wang
    • Yiling Hou
    • Wanru Hou
    • Changwu Yue
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, China West Normal University, Nanchong, Sichuan 637009, P.R. China, National Center for Sweet Potato Improvement Centre of Nanchong, Nanchong Academy of Agricultural Sciences, Nanchong, Sichuan 637001, P.R. China, Key Laboratory of Characteristic Microbial Resources & Drug Development of Guizhou Provincial Education Department, Zunyi Medical University, Zunyi, Guizhou 561000, P.R. China
  • Pages: 3947-3954
    |
    Published online on: August 30, 2016
       https://doi.org/10.3892/mmr.2016.5693
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

A heteropolysaccharide was isolated from the fruiting bodies of Amanita caesarea using a diethylaminoethyl-cellulose column, Sephacryl S‑300 gel column and Sephadex G‑200 column. The Amanita caesarea polysaccharide was predominantly composed of α-D-glucose and α-D-lyxose at a ratio of 2:1, and it had a molecular weight of 19,329 Da. The structural features of the Amanita caesarea polysaccharide were investigated by a combination of total hydrolysis, methylation analysis, gas chromatography-mass spectrometry, and infrared spectra and nuclear magnetic resonance spectroscopy. The results showed that Amanita caesarea polysaccharide (termed AC‑1) had a backbone of 1,4‑linked α‑D‑glucose and 1,3,6‑linked α‑D‑glucose, with branches of one 1‑linked α‑D‑lyxose residue. The antioxidant activity of AC‑1 was evaluated by two biochemical methods, 2,2-azino-bis diammonium (ABTS+) radical scavenging activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH-) radical scavenging activity. The uncontrolled production of free radicals is involved in various diseases, including cancer, atherosclerosis and degenerative aging processes. The results indicated that the Amanita caesarea polysaccharide exhibits strong antioxidant activity, thus, it may be a useful natural product antioxidant.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Hibbett DS, Binder M, Bischoff JF, Blackwell M, Cannon PF, Eriksson OE, Huhndorf S, James T, Kirk PM, Lücking R, et al: A higher-level phylogenetic classification of the fungi. Mycol Res. 111:509–547. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Bertozzi CR and Kiessling LL: Chemical glycobioloy. Science. 291:2357–2364. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Bittencourt VC, Figueiredo RT, da Silva RB, Mourão-Sá DS, Fernandez PL, Sassaki GL, Mulloy B, Bozza MT and Barreto-Bergter E: An alpha-glucan of Pseudallescheria boydii is involved in fungal phagocytosis and toll-like receptor activation. J Biol Chem. 281:22614–22623. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Borchers AT, Stern JS, Hackman RM, Keen CL and Gershwin ME: Mushrooms, tumors, and immunity. Proc Soc Exp Biol Med. 221:281–293. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Rudd PM, Elliott T, Cresswell P, Wilson IA and Dwek RA: Glycosylation and the immune system. Science. 291:2370–2376. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Brown GD, Herre J, Williams DL, Willment JA, Marshall AS and Gordon S: Dectin-1 mediates the biological effects of beta-glucans. J Exp Med. 197:1119–1124. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Angeli JP, Ribeiro LR, Gonzaga ML, Soares Sde A, Ricardo MP, Tsuboy MS, Stidl R, Knasmueller S, Linhares RE and Mantovani MS: Protective effects of beta-glucan extracted from Agaricus brasiliensis against chemically induced DNA damage in human lymphocytes. Cell Biol Toxicol. 22:285–291. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Cordero RJ, Frases S, Guimaräes AJ, Rivera J and Casadevall A: Evidence for branching in cryptococcal capsular polysaccharides and consequences on its biological activity. Mol Microbiol. 79:1101–1117. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Wang F, Hou Y, Ding X, Hou W, Song B, Wang T, Li J and Zeng Y: Structure elucidation and antioxidant effect of a polysaccharide from Lactarius camphoratum (Bull.). Fr Int J Biol Macromol. 62:131–136. 2013. View Article : Google Scholar

10 

Wu XM and Tu PF: Isolation and characterization of alpha-(1→6)-glucans from Cistanche deserticola. J Asian Nat Prod Res. 7:823–828. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Staub AM: Removal of protein-Sevag method. Carbohydr Chem. 5:5–6. 1965.

12 

Kumar CG, Joo HS, Choi JW, Koo YM and Chang CS: Purification and characterization of extracellular polysaccharide from haloalkalophilic Bacillus sp I-450. Enzyme Microb Technol. 34:673–681. 2004. View Article : Google Scholar

13 

Pang XB, Yao WB, Yang XB, Xie C, Liu D, Zhang J and Gao XD: Purification, characterization and biological activity on hepatocytes of a polysaccharide from Flammulina velutipes mycelium. Carbohydr Res. 70:291–297. 2007. View Article : Google Scholar

14 

Yu RM, Yin Y, Yang W, Ma WL, Yang L, Chen XJ, Zhang Z, Ye B and Song LY: Structural elucidation and biological activity of a novel polysaccharide by alkaline extraction from cultured Cordyceps militaris. Carbohydr Polym. 75:166–171. 2009. View Article : Google Scholar

15 

Fan Y, He X, Zhou S, Luo A, He T and Chun Z: Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum. Int J Biol Macromol. 45:169–173. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Sun Y and Liu J: Purification, structure and immunobiological activity of a water-soluble polysaccharide from the fruiting body of Pleurotus ostreatus. Bioresour Technol. 100:983–986. 2009. View Article : Google Scholar

17 

Rongmin Y, Yin Y, Wei Yang, Weili M, Lin Y, Xiujuan C, Zhang Z, Bin Y and Liyan S: Structural elucidation and biological activity of a novel polysaccharide by alkaline extraction from cultured Cordyceps militaris. Carbohydr Polymers. 75:166–171. 2009. View Article : Google Scholar

18 

Ding X, Hou YL and Hou WR: Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Boletus speciosus Forst. Int J Biol Macromol. 50:613–618. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Chen J, Hu T and Zheng R: Antioxidant activities of Sophora subprosrate polysaccharide in immunosuppressed mice. Int Immunopharmacol. 7:547–553. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Luo DH: Identification of structure and antioxidant activity of a fraction of polysaccharide purified from Dioscorea nipponica Makino. Carbohydr Polym. 71:544–549. 2008. View Article : Google Scholar

21 

Kumar V, Lemos M, Sharma M and Shriram V: Antioxidant and DNA damage protecting activities of Eulophia nuda Lindl. Free Radicals and Antioxidants. 3:55–60. 2013. View Article : Google Scholar

22 

Blois MS: Antioxidant determination by use of stable free radicals. Nature. 29:1199–1200. 1958. View Article : Google Scholar

23 

Hertog MGL, Feskens EJM, Kromhout D, Hertog MGL, Hollman PCH, Hertog MGL and Katan MB: Dietary antioxidant flavonoids and risk of coronary heart disease: The Zutphen Elderly Study. Lancet. 342:1007–1011. 1993. View Article : Google Scholar : PubMed/NCBI

24 

Kushi LH, Folsom AR, Prineas RJ, Mink PJ, Wu Y and Bostick RM: Dietary antioxidant vitamins and death from coronary heart disease in postmenopausal women. New Engl J Med. 334:1156–1162. 1996. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhu Y, Ding X, Wang M, Hou Y, Hou W and Yue C: Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea. Mol Med Rep 14: 3947-3954, 2016.
APA
Zhu, Y., Ding, X., Wang, M., Hou, Y., Hou, W., & Yue, C. (2016). Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea. Molecular Medicine Reports, 14, 3947-3954. https://doi.org/10.3892/mmr.2016.5693
MLA
Zhu, Y., Ding, X., Wang, M., Hou, Y., Hou, W., Yue, C."Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea". Molecular Medicine Reports 14.4 (2016): 3947-3954.
Chicago
Zhu, Y., Ding, X., Wang, M., Hou, Y., Hou, W., Yue, C."Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea". Molecular Medicine Reports 14, no. 4 (2016): 3947-3954. https://doi.org/10.3892/mmr.2016.5693
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu Y, Ding X, Wang M, Hou Y, Hou W and Yue C: Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea. Mol Med Rep 14: 3947-3954, 2016.
APA
Zhu, Y., Ding, X., Wang, M., Hou, Y., Hou, W., & Yue, C. (2016). Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea. Molecular Medicine Reports, 14, 3947-3954. https://doi.org/10.3892/mmr.2016.5693
MLA
Zhu, Y., Ding, X., Wang, M., Hou, Y., Hou, W., Yue, C."Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea". Molecular Medicine Reports 14.4 (2016): 3947-3954.
Chicago
Zhu, Y., Ding, X., Wang, M., Hou, Y., Hou, W., Yue, C."Structure and antioxidant activity of a novel polysaccharide derived from Amanita caesarea". Molecular Medicine Reports 14, no. 4 (2016): 3947-3954. https://doi.org/10.3892/mmr.2016.5693
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team