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Article

S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts

  • Authors:
    • Dong Li
    • Kaihua Li
    • Gang Chen
    • Jianlong Xia
    • Ting Yang
    • Ping Cai
    • Chen Yao
    • Yongjiang Yang
    • Shichang Yan
    • Rihua Zhang
    • Hui Chen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing, Jiangsu 210029, P.R. China, Department of Orthopedics, Benq Hospital, Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210019, P.R. China, The Laboratory Animal Center, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029; P.R. China
  • Pages: 3878-3886
    |
    Published online on: September 1, 2016
       https://doi.org/10.3892/mmr.2016.5697
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Abstract

S100 calcium-binding protein B (S100B) is expressed and released by adipocytes, and is positively correlated with body mass index, however, the direct effects of S100B on adipocytes remain unclear. Bone marrow‑derived mesenchymal stem cells have the capacity to differentiate into osteoblasts and adipocytes, which is important for bone metabolism. The current study aimed to determine the effect of S100B on adipogenesis and osteogenesis. The mouse embryo cell line C3H/10T1/2 was used to build cell models with varying levels of S100B protein expression. Western blot analysis was performed to assess the expression of various marker proteins. Oil red O staining and alizarin red S staining were used to detect adipogenesis and osteogenesis, respectively. S100B overexpression was associated with a significant increase in oil red O staining and a significant reduction in alizarin red S staining. Runt‑related transcription factor‑2 and bone morphogenetic protein 2 expression levels were significantly increased in the S100B underexpression group, however not in the S100B overexpression group. By contrast, the expression levels of the adipogenesis markers peroxisome proliferator‑activated receptor γ and CCAAT‑enhancer‑binding protein α was significantly increased in the S100B overexpression group, however not in the S100B underexpression group. Osteogenesis stimulation increased extracellular signal‑regulated kinase (ERK) phosphorylation, and adipogenesis stimulation increased c‑Jun N‑terminal kinase (JNK) phosphorylation. The results suggest that S100B inhibits osteogenesis, however stimulates adipogenesis. The ERK pathway is involved in the regulation of osteogenesis, whereas the JNK pathway is involved in the regulation of adipogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Li D, Li K, Chen G, Xia J, Yang T, Cai P, Yao C, Yang Y, Yan S, Zhang R, Zhang R, et al: S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts. Mol Med Rep 14: 3878-3886, 2016.
APA
Li, D., Li, K., Chen, G., Xia, J., Yang, T., Cai, P. ... Chen, H. (2016). S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts. Molecular Medicine Reports, 14, 3878-3886. https://doi.org/10.3892/mmr.2016.5697
MLA
Li, D., Li, K., Chen, G., Xia, J., Yang, T., Cai, P., Yao, C., Yang, Y., Yan, S., Zhang, R., Chen, H."S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts". Molecular Medicine Reports 14.4 (2016): 3878-3886.
Chicago
Li, D., Li, K., Chen, G., Xia, J., Yang, T., Cai, P., Yao, C., Yang, Y., Yan, S., Zhang, R., Chen, H."S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts". Molecular Medicine Reports 14, no. 4 (2016): 3878-3886. https://doi.org/10.3892/mmr.2016.5697
Copy and paste a formatted citation
x
Spandidos Publications style
Li D, Li K, Chen G, Xia J, Yang T, Cai P, Yao C, Yang Y, Yan S, Zhang R, Zhang R, et al: S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts. Mol Med Rep 14: 3878-3886, 2016.
APA
Li, D., Li, K., Chen, G., Xia, J., Yang, T., Cai, P. ... Chen, H. (2016). S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts. Molecular Medicine Reports, 14, 3878-3886. https://doi.org/10.3892/mmr.2016.5697
MLA
Li, D., Li, K., Chen, G., Xia, J., Yang, T., Cai, P., Yao, C., Yang, Y., Yan, S., Zhang, R., Chen, H."S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts". Molecular Medicine Reports 14.4 (2016): 3878-3886.
Chicago
Li, D., Li, K., Chen, G., Xia, J., Yang, T., Cai, P., Yao, C., Yang, Y., Yan, S., Zhang, R., Chen, H."S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts". Molecular Medicine Reports 14, no. 4 (2016): 3878-3886. https://doi.org/10.3892/mmr.2016.5697
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