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Article

Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts

  • Authors:
    • Wendong Ruan
    • Yuan Xue
    • Yaqi Zong
    • Chao Sun
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The General Hospital of Tianjin Medical University, Tianjin 300052, P.R. China
  • Pages: 4328-4334
    |
    Published online on: September 12, 2016
       https://doi.org/10.3892/mmr.2016.5734
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Abstract

In the present study, third‑generation autologous‑inactivated bone morphogenic protein 2 (BMP2), BMP4, BMP6, BMP7, BMP9 and Wnt3a lentiviral vectors were constructed and integrated into the genome of MC3T3‑E1 murine mesenchymal stem cells (MMSCs) to produce osteoinductive factor gene‑modified MMSCs. The transfection efficiency of each osteoinductive factor was then determined by detecting the expression levels of runt related transcription factor 2 (Runx2) mRNA. The cotransfection with combinations of two lentiviruses was performed, and the expression levels of bone γ‑carboxyglutamate protein and alkaline phosphatase in the MC3T3‑E1 cell culture supernatant were detected. The expression level of Runx2 mRNA was detected by reverse transcription‑polymerase chain reaction, and western blotting was performed to detect the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a. The results demonstrated that the recombinant lentiviruses were successfully transfected into MC3T3‑E1 cells. The relative expression levels of Runx2 mRNA were greatest in the BMP2 group, sequentially followed by the BMP4, BMP9, BMP7, Wnt3a and BMP6 groups. The results of cotransfection of MC3T3‑E1 cells (a total of 8 groups) demonstrated that BMP‑2 and BMP‑7 exhibited the highest cotransfection efficiency. Western blot analysis demonstrated that following BMP2 and BMP7 cotransfection of MC3T3‑E1 cells, the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a were increased compared with control cells. In conclusion, the third‑generation lentiviral vectors effectively improved the osteogenic efficiencies of MC3T3‑E1 cells, which provided an important theoretical basis and therapeutic strategy for bone reconstruction and tissue engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Ruan W, Xue Y, Zong Y and Sun C: Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts. Mol Med Rep 14: 4328-4334, 2016.
APA
Ruan, W., Xue, Y., Zong, Y., & Sun, C. (2016). Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts. Molecular Medicine Reports, 14, 4328-4334. https://doi.org/10.3892/mmr.2016.5734
MLA
Ruan, W., Xue, Y., Zong, Y., Sun, C."Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts". Molecular Medicine Reports 14.5 (2016): 4328-4334.
Chicago
Ruan, W., Xue, Y., Zong, Y., Sun, C."Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts". Molecular Medicine Reports 14, no. 5 (2016): 4328-4334. https://doi.org/10.3892/mmr.2016.5734
Copy and paste a formatted citation
x
Spandidos Publications style
Ruan W, Xue Y, Zong Y and Sun C: Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts. Mol Med Rep 14: 4328-4334, 2016.
APA
Ruan, W., Xue, Y., Zong, Y., & Sun, C. (2016). Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts. Molecular Medicine Reports, 14, 4328-4334. https://doi.org/10.3892/mmr.2016.5734
MLA
Ruan, W., Xue, Y., Zong, Y., Sun, C."Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts". Molecular Medicine Reports 14.5 (2016): 4328-4334.
Chicago
Ruan, W., Xue, Y., Zong, Y., Sun, C."Effect of BMPs and Wnt3a co-expression on the osteogenetic capacity of osteoblasts". Molecular Medicine Reports 14, no. 5 (2016): 4328-4334. https://doi.org/10.3892/mmr.2016.5734
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