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Article

MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma

  • Authors:
    • Zhong‑Jiao Zhu
    • Peng Huang
    • Yan‑Xue Chong
    • Li‑Xin Kang
    • Xing Huang
    • Zhong‑Xiu Zhu
    • Lin Nie
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Tengzhou Central People's Hospital, Tengzhou, Shandong 277500, P.R. China, Department of Emergency, Tengzhou Hospital of Traditional Chinese Medicine, Tengzhou, Shandong 277500, P.R. China, Department of Pharmacy Intravenous Admixture Service, Tengzhou Central People's Hospital, Tengzhou, Shandong 277500, P.R. China, Department of Oncology, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan, Shandong 250031, P.R. China, Department of Orthopedics, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
  • Pages: 4271-4278
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    Published online on: September 14, 2016
       https://doi.org/10.3892/mmr.2016.5741
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Abstract

MicroRNA-181a (miR-181a) is upregulated in osteosarcoma, and its overexpression promotes the proliferation and inhibits the apoptosis of osteosarcoma cells. However, the mechanism of miR‑181a as an oncogene remains to be fully elucidated in osteosarcoma. Cleavage factor (CF) Im25 links alternative polyadenylation to glioblastoma tumor suppression, however, its role in osteosarcoma has not been reported. In the present study, it was confirmed that the expression of miR‑181a was upregulated in osteosarcoma, and that silencing miR‑181a inhibited the proliferation and promoted the apoptosis of osteosarcoma cells. miRNAs are short non‑coding RNAs, which regulate target mRNAs by binding predominantly to the 3'untranslated region (3'UTR), inducing either translational repression or degradation of the target. In the present study, target genes of miR‑181a were screened using miRanda, which is a commonly used prediction algorithm. It was found that miR‑181a targeted the 3'UTR of CFIm25 mRNA. Subsequent experiments confirmed that miR‑181a downregulated the expression of CFIm25 in osteosarcoma cells. Finally, it was demonstrated that the CFIm25 protein was also downregulated in osteosarcoma tissues, and inhibited the proliferation and promoted the apoptosis of the cells. Elucidating the roles of miR‑181a and CFIm25 in osteosarcoma not only assists in further understanding the pathogenesis and progression of this disease, but also offers novel targets for effective therapies.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu ZJ, Huang P, Chong YX, Kang LX, Huang X, Zhu ZX and Nie L: MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma. Mol Med Rep 14: 4271-4278, 2016.
APA
Zhu, Z., Huang, P., Chong, Y., Kang, L., Huang, X., Zhu, Z., & Nie, L. (2016). MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma. Molecular Medicine Reports, 14, 4271-4278. https://doi.org/10.3892/mmr.2016.5741
MLA
Zhu, Z., Huang, P., Chong, Y., Kang, L., Huang, X., Zhu, Z., Nie, L."MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma". Molecular Medicine Reports 14.5 (2016): 4271-4278.
Chicago
Zhu, Z., Huang, P., Chong, Y., Kang, L., Huang, X., Zhu, Z., Nie, L."MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma". Molecular Medicine Reports 14, no. 5 (2016): 4271-4278. https://doi.org/10.3892/mmr.2016.5741
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu ZJ, Huang P, Chong YX, Kang LX, Huang X, Zhu ZX and Nie L: MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma. Mol Med Rep 14: 4271-4278, 2016.
APA
Zhu, Z., Huang, P., Chong, Y., Kang, L., Huang, X., Zhu, Z., & Nie, L. (2016). MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma. Molecular Medicine Reports, 14, 4271-4278. https://doi.org/10.3892/mmr.2016.5741
MLA
Zhu, Z., Huang, P., Chong, Y., Kang, L., Huang, X., Zhu, Z., Nie, L."MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma". Molecular Medicine Reports 14.5 (2016): 4271-4278.
Chicago
Zhu, Z., Huang, P., Chong, Y., Kang, L., Huang, X., Zhu, Z., Nie, L."MicroRNA-181a promotes proliferation and inhibits apoptosis by suppressing CFIm25 in osteosarcoma". Molecular Medicine Reports 14, no. 5 (2016): 4271-4278. https://doi.org/10.3892/mmr.2016.5741
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