Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages

Li,Hong‑Yan; Su,Yan‑Yan; Zhang,Yun‑Fang; Liu,Zhi‑Qiang; Hua,Bao‑Jun

doi:10.3892/mmr.2016.5742

Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages

Authors:
- Hong‑Yan Li
- Yan‑Yan Su
- Yun‑Fang Zhang
- Zhi‑Qiang Liu
- Bao‑Jun Hua
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Affiliations: Department of Nephrology, Huadu District People's Hospital, Southern Medical University, Guangzhou, Guangdong 510800, P.R. China, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
Published online on: September 15, 2016 https://doi.org/10.3892/mmr.2016.5742
Pages: 4055-4062
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ischemia‑reperfusion (I/R) injury is important in the pathogenesis and/or progression of various diseases, including stroke, cardiovascular disease and acute renal injury. Increasing evidence indicates that atorvastatin exerts protective effects in I/R injury‑associated diseases; however, the underlying mechanisms remain to be fully elucidated. In the present study, oxygen‑glucose deprivation (OGD)/reperfusion‑stimulated. RAW264.7 murine macrophages served as a model of I/R injury. The knockdown of peroxisome proliferator activated receptor‑γ (PPARγ) expression in these cells increased OGD/reperfusion‑induced expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor‑α (TNF‑α) and interferon‑γ (IFN‑γ), and enhanced OGD/reperfusion‑induced downregulation of the expression of cluster of differentiation (CD) 206, at the mRNA and protein levels. Conversely, overexpression of PPARγ significantly attenuated OGD/reperfusion‑induced alterations in the expression of iNOS, TNF‑α, IFN‑γ and CD206 at the mRNA and protein levels. Notably, atorvastatin inhibited OGD/reperfusion‑induced iNOS expression and reversed OGD/reperfusion‑induced downregulation of the expression of CD206 and PPARγ at the mRNA and protein levels. The results of the present study indicate that atorvastatin exhibits significant anti‑inflammatory effects in OGD/reperfusion‑stimulated RAW264.7 cells, possibly via PPARγ regulation. The findings of the present study may reveal a novel mechanism underlying the protective effects of atorvastatin in I/R injury‑associated diseases.

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