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Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages

  • Authors:
    • Hong‑Yan Li
    • Yan‑Yan Su
    • Yun‑Fang Zhang
    • Zhi‑Qiang Liu
    • Bao‑Jun Hua
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Huadu District People's Hospital, Southern Medical University, Guangzhou, Guangdong 510800, P.R. China, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4055-4062
    |
    Published online on: September 15, 2016
       https://doi.org/10.3892/mmr.2016.5742
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Abstract

Ischemia‑reperfusion (I/R) injury is important in the pathogenesis and/or progression of various diseases, including stroke, cardiovascular disease and acute renal injury. Increasing evidence indicates that atorvastatin exerts protective effects in I/R injury‑associated diseases; however, the underlying mechanisms remain to be fully elucidated. In the present study, oxygen‑glucose deprivation (OGD)/reperfusion‑stimulated. RAW264.7 murine macrophages served as a model of I/R injury. The knockdown of peroxisome proliferator activated receptor‑γ (PPARγ) expression in these cells increased OGD/reperfusion‑induced expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor‑α (TNF‑α) and interferon‑γ (IFN‑γ), and enhanced OGD/reperfusion‑induced downregulation of the expression of cluster of differentiation (CD) 206, at the mRNA and protein levels. Conversely, overexpression of PPARγ significantly attenuated OGD/reperfusion‑induced alterations in the expression of iNOS, TNF‑α, IFN‑γ and CD206 at the mRNA and protein levels. Notably, atorvastatin inhibited OGD/reperfusion‑induced iNOS expression and reversed OGD/reperfusion‑induced downregulation of the expression of CD206 and PPARγ at the mRNA and protein levels. The results of the present study indicate that atorvastatin exhibits significant anti‑inflammatory effects in OGD/reperfusion‑stimulated RAW264.7 cells, possibly via PPARγ regulation. The findings of the present study may reveal a novel mechanism underlying the protective effects of atorvastatin in I/R injury‑associated diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Li HY, Su YY, Zhang YF, Liu ZQ and Hua BJ: Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages. Mol Med Rep 14: 4055-4062, 2016.
APA
Li, H., Su, Y., Zhang, Y., Liu, Z., & Hua, B. (2016). Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages. Molecular Medicine Reports, 14, 4055-4062. https://doi.org/10.3892/mmr.2016.5742
MLA
Li, H., Su, Y., Zhang, Y., Liu, Z., Hua, B."Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages". Molecular Medicine Reports 14.5 (2016): 4055-4062.
Chicago
Li, H., Su, Y., Zhang, Y., Liu, Z., Hua, B."Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages". Molecular Medicine Reports 14, no. 5 (2016): 4055-4062. https://doi.org/10.3892/mmr.2016.5742
Copy and paste a formatted citation
x
Spandidos Publications style
Li HY, Su YY, Zhang YF, Liu ZQ and Hua BJ: Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages. Mol Med Rep 14: 4055-4062, 2016.
APA
Li, H., Su, Y., Zhang, Y., Liu, Z., & Hua, B. (2016). Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages. Molecular Medicine Reports, 14, 4055-4062. https://doi.org/10.3892/mmr.2016.5742
MLA
Li, H., Su, Y., Zhang, Y., Liu, Z., Hua, B."Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages". Molecular Medicine Reports 14.5 (2016): 4055-4062.
Chicago
Li, H., Su, Y., Zhang, Y., Liu, Z., Hua, B."Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages". Molecular Medicine Reports 14, no. 5 (2016): 4055-4062. https://doi.org/10.3892/mmr.2016.5742
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