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Article

Cysteine‑rich 61 RNA interference inhibits pathological angiogenesis via the phosphatidylinositol 3‑kinase/Akt‑vascular endothelial growth factor signaling pathway in endothelial cells

  • Authors:
    • Yu Di
    • Yiou Zhang
    • Linping Hui
    • Hongwei Yang
    • Yang Yang
    • Aiyuan Wang
    • Xiaolong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Shengjing Affiliated Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Graduate School, China Medical University, Shenyang, Liaoning 110004, P.R. China, Laboratory Center, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 4321-4327
    |
    Published online on: September 26, 2016
       https://doi.org/10.3892/mmr.2016.5772
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Abstract

Hypoxia is a key factor in the pathogenesis of angiogenesis, and cysteine‑rich 61 (CCN1), an angiogenic factor, is involved in the development of pathological angiogenesis. The aim of the present study was to investigate the mechanism of CCN1 RNA interference (RNAi)‑induced inhibition of hypoxia‑induced pathological angiogenesis in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were cultured under hypoxic conditions in vitro. The effects of inhibiting phosphoinositide 3‑kinase (PI3K)/Akt signaling using LY294002 were investigated in hypoxic HUVECs. The proliferation and apoptosis of HUVECs under hypoxia were assessed using CCN1 RNAi. The CCN1‑PI3K/Akt‑vascular endothelial growth factor (VEGF) pathway was analyzed under hypoxic conditions using reverse transcription‑quantitative polymerase chain reaction and western blotting. CCN1 RNAi inhibited the proliferation and induced the apoptosis of the HUVECs under hypoxia, with hypoxia significantly increasing the mRNA and protein expression levels of CCN1, Akt and VEGF. By contrast, CCN1 RNAi reduced the mRNA and protein expression levels of CCN1, Akt and VEGF in the HUVECs (P<0.05). Furthermore, LY294002 reduced the mRNA and protein expression levels of CCN1 in the hypoxic cells (P<0.05). These data indicated that CCN1 inhibits apoptosis and promotes proliferation in HUVECs. Therefore, CCN1 RNAi may offer a novel therapeutic strategy, which may aid in the treatment of pathological angiogenesis via inhibition of the PI3K/Akt‑VEGF pathway.
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View References

1 

Nyengaard JR, Ido Y, Kilo C and Williamson JR: Interactions between hyperglycemia and hypoxia: Implications for diabetic retinopathy. Diabetes. 53:2931–2938. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Chen J and Smith LE: Retinopathy of prematurity. Angiogenesis. 10:133–140. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Leask A and Abraham DJ: All in the CCN family: Essential matricellular signaling modulators emerge from the bunker. J Cell Sci. 119:4803–4810. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Jun JI and Lau LF: The matricellular protein CCN1 induces fibroblast senescence and restricts fibrosis in cutaneous wound healing. Nat Cell Biol. 12:676–685. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Yan L and Chaqour B: Cysteine-rich protein 61 (CCN1) and connective tissue growth factor (CCN2) at the crosshairs of ocular neovascular and fibrovascular disease therapy. J Cell Commun Signal. 7:253–263. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Chintalapudi MR, Markiewicz M, Kose N, Dammai V, Champion KJ, Hoda RS, Trojanowska M and Hsu T: Cyr61/CCN1 and CTGF/CCN2 mediate the proangiogenic activity of VHL-mutant renal carcinoma cells. Carcinogenesis. 29:696–703. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Meyuhas R, Pikarsky E, Tavor E, Klar A, Abramovitch R, Hochman J, Lago TG and Honigman A: A Key role for cyclic AMP-responsive element binding protein in hypoxia-mediated activation of the angiogenesis factor CCN1 (CYR61) in Tumor cells. Mol Cancer Res. 6:1397–1409. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Schmitz P, Gerber U, Schütze N, Jüngel E, Blaheta R, Naggi A, Torri G and Bendas G: Cyr61 is a target for heparin in reducing MV3 melanoma cell adhesion and migration via the integrin VLA-4. Thromb Haemost. 110:1046–1054. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Haque I, De A, Majumder M, Mehta S, McGregor D, Banerjee SK, Van Veldhuizen P and Banerjee S: The matricellular protein CCN1/Cyr61 is a critical regulator of Sonic Hedgehog in pancreatic carcinogenesis. J Biol Chem. 287:38569–38579. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Long QZ, Zhou M, Liu XG, Du YF, Fan JH, Li X and He DL: Interaction of CCN1 with αvβ3 integrin induces P-glycoprotein and confers vinblastine resistance in renal cell carcinoma cells. Anticancer Drugs. 24:810–817. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Emre Y and Imhof BA: Matricellular protein CCN1/CYR61: A new player in inflammation and leukocyte trafficking. Semin Immunopathol. 36:253–259. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Yang XM, Wang YS, Zhang J, Li Y, Xu JF, Zhu J, Zhao W, Chu DK and Wiedemann P: Role of PI3K/Akt and MEK/ERK in mediating hypoxia-induced expression of HIF-1alpha and VEGF in laser-induced rat choroidal neovascularization. Invest Ophthalmol Vis Sci. 50:1873–1879. 2009. View Article : Google Scholar : PubMed/NCBI

13 

You JJ, Yang CH, Yang CM and Chen MS: Cyr61 induces the expression of monocyte chemoattractant protein-1 via the integrin ανβ3, FAK, PI3K/Akt, and NF-κB pathways in retinal vascular endothelial cells. Cell Signal. 26:133–140. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Koon HW, Shih DQ, Hing TC, Chen J, Ho S, Zhao D, Targan SR and Pothoulakis C: Substance P induces CCN1 expression via histone deacetylase activity in human colonic epithelial cells. Am J Pathol. 179:2315–2326. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Shen WG, Peng WX, Shao Y, Xu JF, Dai G, Zhang Y, Pan FY and Li CJ: Localization and activity of calmodulin is involved in cell-cell adhesion of tumor cells and endothelial cells in response to hypoxic stress. Cell Biol Toxicol. 23:323–335. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Liu Y, Zhao T, Yang Z and Li Q: CX3CR1 RNAi inhibits hypoxia-induced microglia activation via p38MAPK/PKC pathway. Int J Exp Pathol. 95:153–157. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Aubry JP, Blaecke A, Lecoanet-Henchoz S, Jeannin P, Herbault N, Caron G, Moine V and Bonnefoy JY: Annexin V used for measuring apoptosis in the early events of cellular cytotoxicity. Cytometry. 37:197–204. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, et al: Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 33:e1792005. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Zou L, Lai H, Zhou Q and Xiao F: Lasting controversy on ranibizumab and bevacizumab. Theranostics. 1:395–402. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Di Y, Zhang YO, Nie QZ and Chen XL: CCN1/Cyr61-PI3K/AKT signaling promotes retinal neovascularization in oxygen-induced retinopathy. Int J Mol Med. 36:1507–1518. 2015.PubMed/NCBI

22 

Brunelle JK and Chandel NS: Oxygen deprivation induced cell death: An update. Apoptosis. 7:475–482. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Jin Y, An X, Ye Z, Cully B, Wu J and Li J: RGS5, a hypoxia-inducible apoptotic stimulator in endothelial cells. J Biol Chem. 284:23436–23443. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Distler JH, Hirth A, Kurowska-Stolarska M, Gay RE, Gay S and Distler O: Angiogenic and angiostatic factors in the molecular control of angiogenesis. Q J Nucl Med. 47:149–161. 2003.PubMed/NCBI

25 

Potente M, Gerhardt H and Carmeliet P: Basic and therapeutic aspects of angiogenesis. Cell. 146:873–887. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Westenskow PD, Kurihara T, Aguilar E, et al: Ras pathway inhibition prevents neovascularization by repressing endothelial cell sprouting. J Clin Invest. 123:4900–4908. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Gergely K and Gerinec A: Retinopathy of prematurity - epidemics, incidence, prevalence, blindness. Bratisl Lek Listy. 111:514–517. 2010.PubMed/NCBI

28 

Grote K, Salguero G, Ballmaier M, Dangers M, Drexler H and Schieffer B: The angiogenic factor CCN1 promotes adhesion and migration of circulating CD34+ progenitor cells: Potential role in angiogenesis and endothelial regeneration. Blood. 110:877–885. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Yu Y, Gao Y, Wang H, Huang L, Qin J, Guo R, Song M, Yu S, Chen J and Cui B: The matrix protein CCN1 (CYR61) promotes proliferation, migration and tube formation of endothelial progenitor cells. Exp Cell Res. 314:3198–3208. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Zhang X, Yu W and Dong F: Cysteine-rich 61 (CYR61) is up-regulated in proliferative diabetic retinopathy. Graefes Arch Clin Exp Ophthalmol. 250:661–668. 2012. View Article : Google Scholar : PubMed/NCBI

31 

You JJ, Yang CH, Chen MS and Yang CM: Cysteine-rich 61, a member of the CCN family, as a factor involved in the pathogenesis of proliferative diabetic retinopathy. Invest Ophthalmol Vis Sci. 50:3447–3455. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Zhou D, Herrick DJ, Rosenbloom J and Chaqour B: Cyr61 mediates the expression of VEGF, alphav-integrin, and alpha-actin genes through cytoskeletally based mechanotransduction mechanisms in bladder smooth muscle cells. J Appl Physiol (1985). 98:2344–2354. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Yang R, Amir J, Liu H and Chaqour B: Mechanical strain activates a program of genes functionally involved in paracrine signaling of angiogenesis. Physiol Genomics. 36:1–14. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Gerszten RE, Friedrich EB, Matsui T, Hung RR, Li L, Force T and Rosenzweig A: Role of phosphoinositide 3-kinase in monocyte recruitment under flow conditions. J Biol Chem. 276:26846–26851. 2001. View Article : Google Scholar : PubMed/NCBI

35 

Kucharzewska P, Welch JE, Svensson KJ and Belting M: The polyamines regulate endothelial cell survival during hypoxic stress through PI3K/AKT and MCL-1. Biochem Biophys Res Commun. 380:413–418. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Hung HS, Wu CC, Chien S and Hsu SH: The behavior of endothelial cells on polyurethane nanocomposites and the associated signaling pathways. Biomaterials. 30:1502–1511. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Huang F, Fang ZF, Hu XQ, Tang L, Zhou SH and Huang JP: Overexpression of miR-126 promotes the differentiation of mesenchymal stem cells toward endothelial cells via activation of PI3K/Akt and MAPK/ERK pathways and release of paracrine factors. Biol Chem. 394:1223–1233. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Lin MT, Kuo IH, Chang CC, Chu CY, Chen HY, Lin BR, Sureshbabu M, Shih HJ and Kuo ML: Involvement of hypoxia-inducing factor-1alpha-dependent plasminogen activator inhibitor-1 up-regulation in Cyr61/CCN1-induced gastric cancer cell invasion. J Biol Chem. 283:15807–15815. 2008. View Article : Google Scholar : PubMed/NCBI

39 

Goodwin CR, Lal B, Zhou X, Ho S, Xia S, Taeger A, Murray J and Laterra J: Cyr61 mediates hepatocyte growth factor-dependent tumor cell growth, migration, and Akt activation. Cancer Res. 70:2932–2941. 2010. View Article : Google Scholar : PubMed/NCBI

40 

Lin J, Huo R, Wang L, Zhou Z, Sun Y, Shen B, Wang R and Li N: A novel anti-Cyr61 antibody inhibits breast cancer growth and metastasis in vivo. Cancer Immunol Immunother. 61:677–687. 2012. View Article : Google Scholar : PubMed/NCBI

41 

You JJ, Yang CM, Chen MS and Yang CH: Regulation of Cyr61/CCN1 expression by hypoxia through cooperation of c-Jun/AP-1 and HIF-1α in retinal vascular endothelial cells. Exp Eye Res. 91:825–836. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Di Y, Zhang Y, Hui L, Yang H, Yang Y, Wang A and Chen X: Cysteine‑rich 61 RNA interference inhibits pathological angiogenesis via the phosphatidylinositol 3‑kinase/Akt‑vascular endothelial growth factor signaling pathway in endothelial cells. Mol Med Rep 14: 4321-4327, 2016.
APA
Di, Y., Zhang, Y., Hui, L., Yang, H., Yang, Y., Wang, A., & Chen, X. (2016). Cysteine‑rich 61 RNA interference inhibits pathological angiogenesis via the phosphatidylinositol 3‑kinase/Akt‑vascular endothelial growth factor signaling pathway in endothelial cells. Molecular Medicine Reports, 14, 4321-4327. https://doi.org/10.3892/mmr.2016.5772
MLA
Di, Y., Zhang, Y., Hui, L., Yang, H., Yang, Y., Wang, A., Chen, X."Cysteine‑rich 61 RNA interference inhibits pathological angiogenesis via the phosphatidylinositol 3‑kinase/Akt‑vascular endothelial growth factor signaling pathway in endothelial cells". Molecular Medicine Reports 14.5 (2016): 4321-4327.
Chicago
Di, Y., Zhang, Y., Hui, L., Yang, H., Yang, Y., Wang, A., Chen, X."Cysteine‑rich 61 RNA interference inhibits pathological angiogenesis via the phosphatidylinositol 3‑kinase/Akt‑vascular endothelial growth factor signaling pathway in endothelial cells". Molecular Medicine Reports 14, no. 5 (2016): 4321-4327. https://doi.org/10.3892/mmr.2016.5772
Copy and paste a formatted citation
x
Spandidos Publications style
Di Y, Zhang Y, Hui L, Yang H, Yang Y, Wang A and Chen X: Cysteine‑rich 61 RNA interference inhibits pathological angiogenesis via the phosphatidylinositol 3‑kinase/Akt‑vascular endothelial growth factor signaling pathway in endothelial cells. Mol Med Rep 14: 4321-4327, 2016.
APA
Di, Y., Zhang, Y., Hui, L., Yang, H., Yang, Y., Wang, A., & Chen, X. (2016). Cysteine‑rich 61 RNA interference inhibits pathological angiogenesis via the phosphatidylinositol 3‑kinase/Akt‑vascular endothelial growth factor signaling pathway in endothelial cells. Molecular Medicine Reports, 14, 4321-4327. https://doi.org/10.3892/mmr.2016.5772
MLA
Di, Y., Zhang, Y., Hui, L., Yang, H., Yang, Y., Wang, A., Chen, X."Cysteine‑rich 61 RNA interference inhibits pathological angiogenesis via the phosphatidylinositol 3‑kinase/Akt‑vascular endothelial growth factor signaling pathway in endothelial cells". Molecular Medicine Reports 14.5 (2016): 4321-4327.
Chicago
Di, Y., Zhang, Y., Hui, L., Yang, H., Yang, Y., Wang, A., Chen, X."Cysteine‑rich 61 RNA interference inhibits pathological angiogenesis via the phosphatidylinositol 3‑kinase/Akt‑vascular endothelial growth factor signaling pathway in endothelial cells". Molecular Medicine Reports 14, no. 5 (2016): 4321-4327. https://doi.org/10.3892/mmr.2016.5772
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