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Article Open Access

Glycogen synthase kinase‑3β is required for epithelial‑mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions

Retraction in: /10.3892/mmr.2020.11523
  • Authors:
    • Jia Guo
    • Lili Yang
    • Yingjin Qiao
    • Zhangsuo Liu
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4091-4098
    |
    Published online on: September 26, 2016
       https://doi.org/10.3892/mmr.2016.5786
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Abstract

Epithelial-mesenchymal transition (EMT) is important for diabetic nephropathy (DN). Podocytes are specialized epithelial cells, which form a major component of the glomerular filtration barrier. Podocyte damage has been suggested to be the primary mechanism behind the albuminuria associated with DN. The present study aimed to determine the function of glycogen synthase kinase (GSK)‑3β in EMT and barrier dysfunction of mouse podocytes exposed to high glucose (HG) conditions. Matured and differentiated podocytes were treated with normal glucose (NG), HG or NG + mannitol. Podocytes were also transfected with a small interfering RNA (siRNA) against GSK‑3β or a scrambled siRNA, or were treated with lithium chloride (LiCl), a GSK‑3β inhibitor, under NG or HG conditions. The expression levels of the epithelial cell markers, nephrin and podocin, and the myofibroblast cell markers, α‑smooth muscle actin (SMA) and fibronectin, in podocytes by western blot analysis and immunofluorescence staining, respectively. The monolayer barrier function was assessed by albumin inflow. The phosphorylation and activity levels of GSK‑3β were also quantified. It was observed that HG promotes EMT in podocytes, due to the increased levels of podocin and nephrin expression and the reduced α‑SMA and fibronectin expression levels. HG also induced barrier dysfunction and increased the expression level of total GSK‑3β, Try216‑phosphorylated‑GSK‑3β and the GSK‑3β activity in podocytes. Transfection of GSK‑3β siRNA or treatment with LiCl reversed the HG‑induced EMT and barrier dysfunction in podocytes. In conclusion, the present study determined that GSK‑3β is required for EMT and barrier dysfunction in podocytes under HG conditions; therefore, GSK‑3β may be a novel target for the treatment of DN.
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Copy and paste a formatted citation
Spandidos Publications style
Guo J, Yang L, Qiao Y and Liu Z: Glycogen synthase kinase‑3β is required for epithelial‑mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions Retraction in /10.3892/mmr.2020.11523. Mol Med Rep 14: 4091-4098, 2016.
APA
Guo, J., Yang, L., Qiao, Y., & Liu, Z. (2016). Glycogen synthase kinase‑3β is required for epithelial‑mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions Retraction in /10.3892/mmr.2020.11523. Molecular Medicine Reports, 14, 4091-4098. https://doi.org/10.3892/mmr.2016.5786
MLA
Guo, J., Yang, L., Qiao, Y., Liu, Z."Glycogen synthase kinase‑3β is required for epithelial‑mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions Retraction in /10.3892/mmr.2020.11523". Molecular Medicine Reports 14.5 (2016): 4091-4098.
Chicago
Guo, J., Yang, L., Qiao, Y., Liu, Z."Glycogen synthase kinase‑3β is required for epithelial‑mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions Retraction in /10.3892/mmr.2020.11523". Molecular Medicine Reports 14, no. 5 (2016): 4091-4098. https://doi.org/10.3892/mmr.2016.5786
Copy and paste a formatted citation
x
Spandidos Publications style
Guo J, Yang L, Qiao Y and Liu Z: Glycogen synthase kinase‑3β is required for epithelial‑mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions Retraction in /10.3892/mmr.2020.11523. Mol Med Rep 14: 4091-4098, 2016.
APA
Guo, J., Yang, L., Qiao, Y., & Liu, Z. (2016). Glycogen synthase kinase‑3β is required for epithelial‑mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions Retraction in /10.3892/mmr.2020.11523. Molecular Medicine Reports, 14, 4091-4098. https://doi.org/10.3892/mmr.2016.5786
MLA
Guo, J., Yang, L., Qiao, Y., Liu, Z."Glycogen synthase kinase‑3β is required for epithelial‑mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions Retraction in /10.3892/mmr.2020.11523". Molecular Medicine Reports 14.5 (2016): 4091-4098.
Chicago
Guo, J., Yang, L., Qiao, Y., Liu, Z."Glycogen synthase kinase‑3β is required for epithelial‑mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions Retraction in /10.3892/mmr.2020.11523". Molecular Medicine Reports 14, no. 5 (2016): 4091-4098. https://doi.org/10.3892/mmr.2016.5786
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