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Article Open Access

Autophagy is involved in aldosterone‑induced mesangial cell proliferation

  • Authors:
    • Min Yang
    • Bin Wang
    • Liying Miao
    • Xianlin Xu
    • Xiaozhou He
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China, Division of Nephrology, Huashan Hospital and Institute of Nephrology, Fudan University, Shanghai 200040, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4638-4642
    |
    Published online on: October 5, 2016
       https://doi.org/10.3892/mmr.2016.5807
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Abstract

The aim of the present study was to investigate whether autophagy is involved in aldosterone (Aldo)-induced mesangial cell (MC) proliferation. MCs were incubated with 10‑7 M Aldo for 24 h. Proliferation of MCs, and the underlying mechanisms, were subsequently analyzed using [3H]thymidine assay, cell counting assay, western blotting and RNA interference (RNAi). Aldo was revealed to induce autophagy, as indicated by the increased conversion from microtubule‑associated protein 1A/1B‑light chain 3 (LC3)‑I to LC3‑II, the increased expression levels of autophagy‑related gene 7 (Atg7) and the increased degradation of p62, which was accompanied by MC proliferation. Notably, pharmacological inhibition of autophagy or RNAi‑mediated knockdown of Atg7 attenuated Aldo‑induced MC proliferation, suggesting that autophagy was at least partially responsible for this effect. The results of the present study provided evidence that autophagy is critical for regulating Aldo‑induced MC proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Yang M, Wang B, Miao L, Xu X and He X: Autophagy is involved in aldosterone‑induced mesangial cell proliferation. Mol Med Rep 14: 4638-4642, 2016.
APA
Yang, M., Wang, B., Miao, L., Xu, X., & He, X. (2016). Autophagy is involved in aldosterone‑induced mesangial cell proliferation. Molecular Medicine Reports, 14, 4638-4642. https://doi.org/10.3892/mmr.2016.5807
MLA
Yang, M., Wang, B., Miao, L., Xu, X., He, X."Autophagy is involved in aldosterone‑induced mesangial cell proliferation". Molecular Medicine Reports 14.5 (2016): 4638-4642.
Chicago
Yang, M., Wang, B., Miao, L., Xu, X., He, X."Autophagy is involved in aldosterone‑induced mesangial cell proliferation". Molecular Medicine Reports 14, no. 5 (2016): 4638-4642. https://doi.org/10.3892/mmr.2016.5807
Copy and paste a formatted citation
x
Spandidos Publications style
Yang M, Wang B, Miao L, Xu X and He X: Autophagy is involved in aldosterone‑induced mesangial cell proliferation. Mol Med Rep 14: 4638-4642, 2016.
APA
Yang, M., Wang, B., Miao, L., Xu, X., & He, X. (2016). Autophagy is involved in aldosterone‑induced mesangial cell proliferation. Molecular Medicine Reports, 14, 4638-4642. https://doi.org/10.3892/mmr.2016.5807
MLA
Yang, M., Wang, B., Miao, L., Xu, X., He, X."Autophagy is involved in aldosterone‑induced mesangial cell proliferation". Molecular Medicine Reports 14.5 (2016): 4638-4642.
Chicago
Yang, M., Wang, B., Miao, L., Xu, X., He, X."Autophagy is involved in aldosterone‑induced mesangial cell proliferation". Molecular Medicine Reports 14, no. 5 (2016): 4638-4642. https://doi.org/10.3892/mmr.2016.5807
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