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Analysis of altered microRNA expression profiles in the kidney tissues of ethylene glycol-induced hyperoxaluric rats

  • Authors:
    • Zhuo Liu
    • Hongyang Jiang
    • Jun Yang
    • Tao Wang
    • Yufeng Ding
    • Jihong Liu
    • Shaogang Wang
    • Zhangqun Ye
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4650-4658
    |
    Published online on: October 12, 2016
       https://doi.org/10.3892/mmr.2016.5833
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Abstract

Calcium oxalate stones account for >80% of urinary stones, however the mechanisms underlying their formation remains to be elucidated. Hyperoxaluria serves an important role in the pathophysiological process of stone formation. In the present study, differences in the miRNA expression profiles between experimental hyperoxaluric rats and normal rats were analyzed, in order to identify target genes and signaling pathways involved in the pathogenesis of hyperoxaluria. Ethylene glycol and ammonium chloride was fed to male hyperoxaluric rats (EXP) and normal age‑matched male rats (CON). The oxalate concentration in the urine of each experimental rat was collected every 24 h and measured on day 14. Three rats exhibiting the highest concentrations were selected for microarray analysis. Microarray analysis was performed to evaluate differences in the expression of microRNA (miRNA) in the kidney tissues from EXP and CON groups, and miRNAs that exhibited a >2‑fold or a <0.5‑fold alteration in expression between these groups were screened for differential expression patterns according to the threshold P‑values. Reverse transcription‑quantitative polymerase chain reaction analysis was employed to confirm the microarray results. In order to predict the potential role of miRNAs in pathophysiological processes, gene ontology (GO), pathway and target prediction analyses were conducted. A total of 28 miRNAs were observed to be differentially expressed (>2‑fold change) between EXP and CON groups. Among these miRNAs, 20 were upregulated and 8 were downregulated. GO and pathway analyses revealed that the insulin resistance and phosphatidylinositol‑bisphosphonate 3‑kinase/AKT serine threonine kinase signaling pathways were potentially associated with miRNA regulation in this setting. In conclusion, the results of the present study identified differentially expressed miRNAs in hyperoxaluric rats, and provided a novel perspective for the role of miRNAs in the formation of calcium oxalate stones.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Z, Jiang H, Yang J, Wang T, Ding Y, Liu J, Wang S and Ye Z: Analysis of altered microRNA expression profiles in the kidney tissues of ethylene glycol-induced hyperoxaluric rats. Mol Med Rep 14: 4650-4658, 2016.
APA
Liu, Z., Jiang, H., Yang, J., Wang, T., Ding, Y., Liu, J. ... Ye, Z. (2016). Analysis of altered microRNA expression profiles in the kidney tissues of ethylene glycol-induced hyperoxaluric rats. Molecular Medicine Reports, 14, 4650-4658. https://doi.org/10.3892/mmr.2016.5833
MLA
Liu, Z., Jiang, H., Yang, J., Wang, T., Ding, Y., Liu, J., Wang, S., Ye, Z."Analysis of altered microRNA expression profiles in the kidney tissues of ethylene glycol-induced hyperoxaluric rats". Molecular Medicine Reports 14.5 (2016): 4650-4658.
Chicago
Liu, Z., Jiang, H., Yang, J., Wang, T., Ding, Y., Liu, J., Wang, S., Ye, Z."Analysis of altered microRNA expression profiles in the kidney tissues of ethylene glycol-induced hyperoxaluric rats". Molecular Medicine Reports 14, no. 5 (2016): 4650-4658. https://doi.org/10.3892/mmr.2016.5833
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Z, Jiang H, Yang J, Wang T, Ding Y, Liu J, Wang S and Ye Z: Analysis of altered microRNA expression profiles in the kidney tissues of ethylene glycol-induced hyperoxaluric rats. Mol Med Rep 14: 4650-4658, 2016.
APA
Liu, Z., Jiang, H., Yang, J., Wang, T., Ding, Y., Liu, J. ... Ye, Z. (2016). Analysis of altered microRNA expression profiles in the kidney tissues of ethylene glycol-induced hyperoxaluric rats. Molecular Medicine Reports, 14, 4650-4658. https://doi.org/10.3892/mmr.2016.5833
MLA
Liu, Z., Jiang, H., Yang, J., Wang, T., Ding, Y., Liu, J., Wang, S., Ye, Z."Analysis of altered microRNA expression profiles in the kidney tissues of ethylene glycol-induced hyperoxaluric rats". Molecular Medicine Reports 14.5 (2016): 4650-4658.
Chicago
Liu, Z., Jiang, H., Yang, J., Wang, T., Ding, Y., Liu, J., Wang, S., Ye, Z."Analysis of altered microRNA expression profiles in the kidney tissues of ethylene glycol-induced hyperoxaluric rats". Molecular Medicine Reports 14, no. 5 (2016): 4650-4658. https://doi.org/10.3892/mmr.2016.5833
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