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Article

Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries

  • Authors:
    • Yubao Cui
    • Lili Yu
    • Ying Zhou
    • Li Yang
    • Chengbo Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Central Laboratory, The Third People's Hospital of Yancheng City, Affiliated Yancheng Hospital, School of Medicine, Southeast University, Yancheng, Jiangsu 224000, P.R. China, Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng, Jiangsu 224006, P.R. China
  • Pages: 4816-4822
    |
    Published online on: October 13, 2016
       https://doi.org/10.3892/mmr.2016.5848
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Abstract

Mimotope mapping enables the characterization of allergen epitopes for the development of diagnostic and therapeutic approaches. In the present study, a phage display peptide library was used for mimotope mapping based on the binding of antibodies against the recombinant group 5 allergen from the house dust mite Dermatophagoides farinae (Der f 5), an arthropod that causes indoor allergies worldwide. When three monoclonal anti‑Der f 5 antibodies were used for biopanning, seven mimotopes were identified. Their common subsequence was ʻ‑‑‑[‑A][‑T]W[‑S]H[HSFW][LM][PSKR] [TLV][AST]‑[DP][‑L]‑ʼ. When analyzed in combination with predicted discontinous epitopes, amino acids P2, K3, K4, H5, F11, F13, L14, R72, T77, L79, R84, T39, F40, P44, T45 and K46 were identified as key residues in conformational epitopes of Der f 5. Therefore, the seven mimotopes or modification of the key amino acids may facilitate the development of blocking antibodies or epitope‑specific immunotherapies for mite allergy.
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Copy and paste a formatted citation
Spandidos Publications style
Cui Y, Yu L, Zhou Y, Yang L and Zhang C: Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries. Mol Med Rep 14: 4816-4822, 2016.
APA
Cui, Y., Yu, L., Zhou, Y., Yang, L., & Zhang, C. (2016). Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries. Molecular Medicine Reports, 14, 4816-4822. https://doi.org/10.3892/mmr.2016.5848
MLA
Cui, Y., Yu, L., Zhou, Y., Yang, L., Zhang, C."Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries". Molecular Medicine Reports 14.5 (2016): 4816-4822.
Chicago
Cui, Y., Yu, L., Zhou, Y., Yang, L., Zhang, C."Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries". Molecular Medicine Reports 14, no. 5 (2016): 4816-4822. https://doi.org/10.3892/mmr.2016.5848
Copy and paste a formatted citation
x
Spandidos Publications style
Cui Y, Yu L, Zhou Y, Yang L and Zhang C: Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries. Mol Med Rep 14: 4816-4822, 2016.
APA
Cui, Y., Yu, L., Zhou, Y., Yang, L., & Zhang, C. (2016). Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries. Molecular Medicine Reports, 14, 4816-4822. https://doi.org/10.3892/mmr.2016.5848
MLA
Cui, Y., Yu, L., Zhou, Y., Yang, L., Zhang, C."Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries". Molecular Medicine Reports 14.5 (2016): 4816-4822.
Chicago
Cui, Y., Yu, L., Zhou, Y., Yang, L., Zhang, C."Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries". Molecular Medicine Reports 14, no. 5 (2016): 4816-4822. https://doi.org/10.3892/mmr.2016.5848
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