Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November-2016 Volume 14 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2016 Volume 14 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference

  • Authors:
    • Juanyong Xu
    • Dandan Zhu
    • Jing Shan
    • Yuan Fan
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4575-4580
    |
    Published online on: October 13, 2016
       https://doi.org/10.3892/mmr.2016.5850
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The major cell types expressing Golli in the immune system are the T‑lineage cells. The aim of the current study was to investigate the changes of gene expression in T lymphocytes subsequent to downregulation of the Golli‑myelin basic protein (MBP) gene. RNA interference technology was used to suppress the expression of Golli‑MBP in Jurkat cells and DNA microarray techniques were applied to investigate the alterations of gene expression profiles. The results indicated that there were 387 differentially expressed genes. In the Golli‑MBP knockdown Jurkat cells, the expression of 108 genes was enhanced, 279 genes were suppressed. Gene ontology analysis identified differentially expressed genes involved in several biological progresses, including cell adhesion and immune responses. Pathway analysis demonstrated that the majority of the differentially expressed genes (23.3%) were involved in cytokine‑cytokine receptor interaction. Subsequent to Golli‑MBP knockdown, the mechanisms that changed the biological characteristics of Jurkat cells were complex, involving numerous types of functional proteins, and metabolic and signaling pathways. However, further experiments are required to confirm these results.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Feng JM: Minireview: Expression and function of golli protein in immune system. Neurochem Res. 32:273–278. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Campagnoni AT, Pribyl TM, Campagnoni CW, Kampf K, Amur-Umarjee S, Landry CF, Handley VW, Newman SL, Garbay B and Kitamura K: Structure and developmental regulation of Golli-mbp, a 105-kilobase gene that encompasses the myelin basic protein gene and is expressed in cells in the oligodendrocyte lineage in the brain. J Biol Chem. 268:4930–4938. 1993.PubMed/NCBI

3 

Pribyl TM, Campagnoni CW, Kampf K, Kashima T, Handley VW, McMahon J and Campagnoni AT: The human myelin basic protein gene is included within a 179-kilobase transcription unit: Expression in the immune and central nervous systems. Proc Natl Acad Sci USA. 90:10695–10699. 1993. View Article : Google Scholar : PubMed/NCBI

4 

Feng JM, Fernandes AO, Campagnoni CW, Hu YH and Campagnoni AT: The golli-myelin basic protein negatively regulates signal transduction in T lymphocytes. J Neuroimmunol. 152:57–66. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Feng JM, Hu YK, Xie LH, Colwell CS, Shao XM, Sun XP, Chen B, Tang H and Campagnoni AT: Golli protein negatively regulates store depletion-induced calcium influx in T cells. Immunity. 24:717–727. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Slavin AJ, Maron R and Weiner HL: Mucosal administration of IL-10 enhances oral tolerance in autoimmune encephalomyelitis and diabetes. Int Immunol. 13:825–833. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Voskuhl RR, Pribyl TM, Kampf K, Handley V, Liu HB, Feng J, Campagnoni CW, Soldan SS, Messing A and Campagnoni AT: Experimental autoimmune encephalomyelitis relapses are reduced in heterozygous golli MBP knockout mice. J Neuroimmunol. 139:44–50. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Nagasato K, Farris RW, Dubois-Dalcq M and Voskuhl RR: Exon 2 containing myelin basic protein (MBP) transcripts are expressed in lesions of experimental allergic encephalomyelitis (EAE). J Neuroimmunol. 72:21–25. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Shiryaev SA, Savinov AY, Cieplak P, Ratnikov BI, Motamedchaboki K, Smith JW and Strongin AY: Matrix metalloproteinase proteolysis of the myelin basic protein isoforms is a source of immunogenic peptides in autoimmune multiple sclerosis. PloS One. 4:e49522009. View Article : Google Scholar : PubMed/NCBI

10 

Ding M, Zeng J, Sroussi H, Yu J, Xu J, Cheng X and Fan Y: Interactions between Golli-MBP and Th1/Th2 cytokines in patients with oral lichen planus. Oral Dis. 20:205–211. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Allen SJ, Crown SE and Handel TM: Chemokine: Receptor structure, interactions and antagonism. Annu Rev Immunol. 25:787–820. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Bennett LD, Fox JM and Signoret N: Mechanisms regulating chemokine receptor activity. Immunology. 134:246–256. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Cheng W and Chen G: Chemokines and chemokine receptors in multiple sclerosis. Mediators Inflamm. 2014:6592062014. View Article : Google Scholar : PubMed/NCBI

14 

Yu SL, Kuan WP, Wong CK, Li EK and Tam LS: Immunopathological roles of cytokines, chemokines, signaling molecules and pattern-recognition receptors in systemic lupus erythematosus. Clin Dev Immunol. 2012:7151902012. View Article : Google Scholar : PubMed/NCBI

15 

Siddiqui S, Secor ER Jr and Silbart LK: Broncho-alveolar macrophages express chemokines associated with leukocyte migration in a mouse model of asthma. Cell Immunol. 281:159–169. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Eisen D: The clinical features, malignant potential and systemic associations of oral lichen planus: A study of 723 patients. J Am Acad Dermatol. 46:207–214. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Mattila R, Ahlfors E and Syrjänen S: CD27 and CD38 lymphocytes are detected in oral lichen planus lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 111:211–217. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Hu JY, Zhang J, Cui JL, Liang XY, Lu R, Du GF, Xu XY and Zhou G: Increasing CCL5/CCR5 on CD4+ T cells in peripheral blood of oral lichen planus. Cytokine. 62:141–145. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Zhao ZZ, Sugerman PB, Walsh LJ and Savage NW: Expression of RANTES and CCR1 in oral lichen planus and association with mast cell migration. J Oral Pathol Med. 31:158–162. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xu J, Zhu D, Shan J and Fan Y: Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference. Mol Med Rep 14: 4575-4580, 2016.
APA
Xu, J., Zhu, D., Shan, J., & Fan, Y. (2016). Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference. Molecular Medicine Reports, 14, 4575-4580. https://doi.org/10.3892/mmr.2016.5850
MLA
Xu, J., Zhu, D., Shan, J., Fan, Y."Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference". Molecular Medicine Reports 14.5 (2016): 4575-4580.
Chicago
Xu, J., Zhu, D., Shan, J., Fan, Y."Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference". Molecular Medicine Reports 14, no. 5 (2016): 4575-4580. https://doi.org/10.3892/mmr.2016.5850
Copy and paste a formatted citation
x
Spandidos Publications style
Xu J, Zhu D, Shan J and Fan Y: Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference. Mol Med Rep 14: 4575-4580, 2016.
APA
Xu, J., Zhu, D., Shan, J., & Fan, Y. (2016). Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference. Molecular Medicine Reports, 14, 4575-4580. https://doi.org/10.3892/mmr.2016.5850
MLA
Xu, J., Zhu, D., Shan, J., Fan, Y."Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference". Molecular Medicine Reports 14.5 (2016): 4575-4580.
Chicago
Xu, J., Zhu, D., Shan, J., Fan, Y."Changes of gene expression in T lymphocytes following Golli‑MBP gene RNA interference". Molecular Medicine Reports 14, no. 5 (2016): 4575-4580. https://doi.org/10.3892/mmr.2016.5850
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team